##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527994_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3077240
Sequences flagged as poor quality	0
Sequence length	59
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.498359893930925	31.0	31.0	34.0	30.0	34.0
2	31.96634809114661	33.0	31.0	34.0	30.0	34.0
3	32.12829483563193	33.0	31.0	34.0	30.0	34.0
4	35.7836977291339	37.0	35.0	37.0	35.0	37.0
5	35.697643342735695	37.0	35.0	37.0	33.0	37.0
6	35.82401827611756	37.0	35.0	37.0	35.0	37.0
7	36.10977369330959	37.0	35.0	37.0	35.0	37.0
8	35.86477330334976	37.0	35.0	37.0	35.0	37.0
9	37.89629635647528	39.0	38.0	39.0	35.0	39.0
10	37.3274811194447	39.0	37.0	39.0	33.0	39.0
11	37.82503347155243	39.0	37.0	39.0	35.0	39.0
12	37.8493328437171	39.0	37.0	39.0	35.0	39.0
13	37.70186985740469	39.0	37.0	39.0	35.0	39.0
14	37.94839466534947	40.0	38.0	40.0	33.0	40.0
15	36.522587773459335	38.0	35.0	40.0	31.0	40.0
16	36.2983345465417	38.0	35.0	40.0	31.0	40.0
17	36.23119613679791	38.0	35.0	40.0	30.0	40.0
18	36.37927948421313	38.0	35.0	40.0	30.0	40.0
19	36.61447205937788	39.0	35.0	40.0	31.0	40.0
20	36.756004406546126	39.0	35.0	40.0	31.0	40.0
21	36.744514240033276	39.0	35.0	40.0	31.0	40.0
22	36.75159493572162	39.0	35.0	40.0	31.0	40.0
23	36.677308562218094	39.0	35.0	40.0	31.0	40.0
24	36.65937268461349	39.0	35.0	40.0	31.0	40.0
25	36.60613861772237	39.0	35.0	40.0	31.0	40.0
26	36.471015260428175	39.0	35.0	40.0	31.0	40.0
27	36.34922365496354	39.0	35.0	40.0	30.0	40.0
28	36.27185789863644	38.0	35.0	40.0	30.0	40.0
29	36.18536513239136	38.0	35.0	40.0	30.0	40.0
30	36.09830464962109	38.0	35.0	40.0	30.0	40.0
31	36.06095624650661	38.0	35.0	40.0	30.0	40.0
32	35.923632865814824	38.0	35.0	40.0	30.0	40.0
33	35.7882888562478	38.0	35.0	40.0	30.0	40.0
34	35.73045001364859	38.0	35.0	40.0	29.0	40.0
35	35.64583197930613	38.0	34.0	40.0	29.0	40.0
36	35.44559410380731	38.0	34.0	40.0	28.0	40.0
37	35.3252879203442	38.0	34.0	40.0	28.0	40.0
38	35.11459229699341	37.0	34.0	40.0	27.0	40.0
39	34.92764002807711	37.0	34.0	40.0	27.0	40.0
40	34.7050594038814	37.0	33.0	40.0	26.0	40.0
41	34.490458332791725	36.0	33.0	40.0	25.0	40.0
42	34.31840935383656	36.0	33.0	40.0	24.0	40.0
43	34.07849014051553	36.0	33.0	40.0	24.0	40.0
44	33.89088338901093	35.0	33.0	40.0	23.0	40.0
45	33.651940700107886	35.0	33.0	39.0	23.0	40.0
46	33.4175839388543	35.0	33.0	39.0	23.0	40.0
47	33.295693543565015	35.0	33.0	39.0	23.0	40.0
48	33.03671764308276	35.0	32.0	39.0	22.0	40.0
49	32.92351977746292	35.0	32.0	38.0	22.0	40.0
50	32.71153696169294	35.0	32.0	38.0	20.0	40.0
51	32.48989581573098	35.0	31.0	38.0	20.0	40.0
52	32.31417374010477	35.0	31.0	38.0	20.0	40.0
53	32.0432819669574	35.0	31.0	37.0	20.0	40.0
54	31.83126957923334	35.0	31.0	37.0	18.0	40.0
55	31.603827455772056	35.0	31.0	37.0	18.0	40.0
56	31.300861486266914	34.0	30.0	36.0	17.0	39.0
57	31.046183268123382	34.0	30.0	36.0	15.0	39.0
58	30.783997673239657	34.0	29.0	36.0	13.0	39.0
59	30.21038885494794	34.0	28.0	35.0	10.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	9.0
14	27.0
15	147.0
16	496.0
17	1109.0
18	2388.0
19	4454.0
20	7337.0
21	11429.0
22	16359.0
23	22259.0
24	29993.0
25	38869.0
26	49359.0
27	60878.0
28	69800.0
29	78339.0
30	91791.0
31	111181.0
32	138010.0
33	179010.0
34	276906.0
35	341887.0
36	322961.0
37	482079.0
38	602145.0
39	138016.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.320917445503113	20.66865112893372	33.889719358906035	16.120712066657134
2	23.828463168293666	22.227385579285333	34.53360803837205	19.410543214048953
3	24.156711858678555	20.825090015728378	36.229185893852936	18.78901223174013
4	28.62298683235627	20.39525678855078	38.91113465313073	12.070621725962226
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	99.1852764165291	0.5083776371033784	0.1697625144610105	0.13658343190651362
12	98.74176209850386	0.6958183307119367	0.36558734450351615	0.1968322262806931
13	93.55929339278055	3.460308588215414	1.7190729354876446	1.2613250835163978
14	53.522117221926145	28.155522481184438	7.57077770989588	10.751582586993539
15	31.745947667390258	29.744803785210124	18.20183021148822	20.3074183359114
16	29.45902822009333	19.46120549583393	27.122486383902455	23.957279900170285
17	22.10666051396706	18.780433115389116	31.899949305221565	27.21295706542226
18	21.75361687746162	21.461017015247428	32.12342878683496	24.661937320455994
19	24.775025672355746	21.477395328281187	28.331686836255866	25.415892163107202
20	25.895152799261677	21.2107927883428	25.85163978110255	27.042414631292978
21	27.736250666181384	22.88193316088443	23.068106485032043	26.313709687902147
22	28.224155411992562	21.43888679466015	22.940394639352146	27.39656315399514
23	26.59639807099869	21.266557044624403	25.078056960133104	27.058987924243805
24	26.209785392104614	23.01481197436664	24.56149016651285	26.2139124670159
25	27.642302842807194	19.742691502775216	25.604762709440926	27.01024294497667
26	28.986234417854963	20.110131156490883	23.90239305351549	27.00124137213867
27	31.18736270164173	22.73560073312449	21.157595767635932	24.919440797597847
28	31.6682156737856	22.275220652272814	21.02744667299268	25.029117000948904
29	33.1988405194265	21.18606283552794	19.98654638572227	25.62855025932329
30	31.778996763333378	23.108077368031093	19.452431399565846	25.660494469069683
31	27.34882557096619	24.219657875238852	20.430450663581652	28.001065890213304
32	28.200042895581756	26.34025945327631	20.168105185165928	25.291592465976002
33	28.097093499369564	26.84964448661788	20.290682559696354	24.762579454316207
34	26.202343658603166	27.37963239786302	21.280693088611873	25.13733085492194
35	28.04435143180252	27.17129635647528	19.28218143531216	25.502170776410033
36	28.55386645175547	27.47075301243972	20.630922514980956	23.344458020823854
37	29.045118352809663	27.048686485292016	20.913740884688877	22.992454277209447
38	28.287393898428466	27.570875199854417	22.427142504322052	21.71458839739507
39	28.335228971415944	28.152435299164186	22.436241567118586	21.076094162301285
40	28.704098477856782	28.225812741287648	20.36315009554016	22.70693868531541
41	28.330224486877853	27.85470096580052	20.24226254695766	23.572812000363964
42	28.95445269137279	28.215446309030167	19.72072376545216	23.109377234144883
43	28.993026218299516	26.86885000844913	19.77600057194109	24.362123201310265
44	27.029091003626625	29.13074703305559	20.182988652168827	23.657173311148952
45	26.933518347610196	27.882615590594167	21.350560892228103	23.833305169567534
46	27.88521532282175	27.03682520700368	20.91757548972456	24.160383980450014
47	29.15745928169399	25.35099634737622	21.120517086740065	24.37102728418973
48	27.063017509196552	26.151128933719825	21.765868115584095	25.019985441499525
49	27.190111918472397	26.373860992317795	21.141607414436315	25.2944196747735
50	27.623487280810078	27.888075028272084	20.30537104678218	24.183066644135653
51	25.284638182267226	28.68482796271984	21.943397330075	24.087136524937932
52	26.13488060729745	28.54044533413058	21.270196669743015	24.05447738882895
53	25.895672745707195	28.19806059975822	21.74773498329672	24.158531671237863
54	25.494111606504532	27.98709232949006	22.014402516540795	24.504393547464613
55	26.04704215465807	27.99381913662893	20.593486370903797	25.36565233780921
56	26.91749749775773	27.614453211319233	21.18482796271984	24.283221328203194
57	27.358509573513928	26.415846667793218	21.992727249093345	24.232916509599512
58	25.943897778528815	26.649822568275468	21.000539444437223	26.4057402087585
59	26.54817303817707	26.1137902796012	22.722927038515035	24.6151096437067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5
7	3.0
8	54.5
9	299.0
10	492.0
11	989.0
12	1486.0
13	1715.5
14	2099.5
15	2254.0
16	2132.0
17	2010.0
18	2067.0
19	2415.5
20	2707.0
21	3184.0
22	3661.0
23	5485.5
24	7310.0
25	10912.0
26	20049.5
27	25585.0
28	31345.0
29	37105.0
30	41669.5
31	46186.5
32	46139.0
33	48469.5
34	50800.0
35	49439.5
36	49381.5
37	50684.0
38	52086.0
39	53488.0
40	58915.0
41	64342.0
42	70039.0
43	81877.0
44	88018.0
45	89385.5
46	90753.0
47	94719.5
48	112646.0
49	126606.0
50	135299.5
51	143993.0
52	151772.5
53	160189.5
54	160827.0
55	184811.0
56	208795.0
57	215625.5
58	235830.0
59	249204.0
60	235582.0
61	221960.0
62	204070.0
63	186180.0
64	172877.0
65	139001.5
66	118429.0
67	103087.5
68	87746.0
69	81198.0
70	65986.5
71	57323.0
72	48430.5
73	39538.0
74	32101.0
75	17024.0
76	9384.0
77	7157.5
78	4931.0
79	4730.0
80	4529.0
81	2724.5
82	1031.5
83	1143.0
84	719.5
85	296.0
86	215.0
87	117.5
88	101.0
89	69.5
90	38.0
91	21.5
92	3.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	3077240.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.51747073440491
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.72603913225929	14.666684402069924
2	6.125799359692155	2.146170220164873
3	2.644216154275844	1.389599372939034
4	1.5487945633053997	1.0852385374523108
5	0.957556621307797	0.8386985045147488
6	0.6540458469051279	0.6874337389271792
7	0.44410462222338276	0.5445712805968438
8	0.33143549489714685	0.4644729265763022
9	0.24257261661550958	0.38243328412771904
>10	1.5914499632242618	5.432698153091831
>50	0.32277130644952207	4.189761575818323
>100	1.2818577297735774	52.27004360082545
>500	0.11915913533404586	13.620547159620527
>1k	0.010197453736986491	2.281647243274931
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.003769611729991811	0.0	0.0	0.0	0.0
12	0.006076874081969557	0.0	0.0	0.0	0.0
13	0.017483199230479262	0.0	0.0	0.0	0.0
14	0.06710558812442319	0.0	0.0	0.0	0.0
15	0.11565558747449013	0.0	0.0	0.0	0.0
16	0.14178289636167474	0.0	0.0	0.0	0.0
17	0.1584536792710351	0.0	0.0	0.0	0.0
18	0.18610833084192327	0.0	0.0	0.0	0.0
19	0.21083828365678337	0.0	0.0	0.0	0.0
20	0.23810297539353448	0.0	0.0	0.0	0.0
21	0.26416529097502955	0.0	0.0	0.0	0.0
22	0.29142998271178067	0.0	0.0	0.0	0.0
23	0.3197670639924088	0.0	0.0	0.0	0.0
24	0.3490465482055348	0.0	0.0	0.0	0.0
25	0.38076328138201765	0.0	0.0	0.0	0.0
26	0.4178419622778854	0.0	0.0	0.0	0.0
27	0.45706542226150704	0.0	0.0	0.0	0.0
28	0.4963213788979735	0.0	0.0	0.0	0.0
29	0.5407443033367563	0.0	0.0	0.0	0.0
30	0.5937788407793997	0.0	0.0	0.0	0.0
31	0.6435962095904122	0.0	0.0	0.0	0.0
32	0.6920487189819449	0.0	0.0	0.0	0.0
33	0.7330594948720282	0.0	0.0	0.0	0.0
34	0.7715355318402205	0.0	0.0	0.0	0.0
35	0.8105965085596184	0.0	0.0	0.0	0.0
36	0.8480001559839336	0.0	0.0	0.0	0.0
37	0.88793854233014	0.0	0.0	0.0	0.0
38	0.9317765270177172	0.0	0.0	0.0	0.0
39	0.9784092238499434	0.0	0.0	0.0	0.0
40	1.0334910504218064	0.0	0.0	0.0	0.0
41	1.0893527966619438	0.0	0.0	0.0	0.0
42	1.151096437066982	0.0	0.0	0.0	0.0
43	1.2147248833370163	0.0	0.0	0.0	0.0
44	1.2779308731200687	0.0	0.0	0.0	0.0
45	1.341136862903121	0.0	0.0	0.0	0.0
46	1.4043428526861734	0.0	0.0	0.0	0.0
47	1.461894424874238	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATCA	20	3.1390728E-4	53.000004	15
TATACGA	40	1.6025297E-9	53.000004	30
TTTAGCG	30	7.014369E-7	53.0	37
CTAGACG	35	3.3445758E-8	53.0	32
CGGCGTA	50	3.6379788E-12	53.0	29
ACTATCG	15	0.006697329	53.0	49
CATATAG	25	1.4793173E-5	53.0	51
ACGTTAT	25	1.4793173E-5	53.0	30
ACGTTAA	60	0.0	53.0	29
ATTACGT	25	1.4793173E-5	53.0	17
TTCGTAA	15	0.006697329	53.0	27
TACGGTA	25	1.4793173E-5	53.0	31
TAACGTG	15	0.006697329	53.0	35
CTAACGG	15	0.006697329	53.0	37
CCGTAGA	35	3.3445758E-8	53.0	37
TTGCGAA	30	7.014369E-7	53.0	52
CATAACG	30	7.014369E-7	53.0	28
TATAGCG	60	0.0	53.0	42
ATGCGAT	35	3.3445758E-8	53.0	49
CACGTTA	25	1.4793173E-5	53.0	29
>>END_MODULE