FastQCFastQC Report
Fri 17 Jun 2016
SRR1527993_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527993_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3681942
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA351.0203985E-746.00000422
TCGTTAA351.0203985E-746.00000433
GTCGATA700.046.00000436
ATAGCCG551.8189894E-1246.00000442
TAACGGG551.8189894E-1246.00000435
TATGCGT351.0203985E-746.00000445
GCCGATA351.0203985E-746.00000415
CGAATCG351.0203985E-746.00000434
TTACGAC551.8189894E-1246.00000434
ACCGTAC700.046.00000443
CGATAGG700.046.00000438
GATACGC301.8625287E-646.013
ACGTTAG501.6370905E-1146.029
ATAATAC253.418634E-546.031
TTCGTAA301.8625287E-646.034
CACGATT206.313277E-446.030
TAAGTCG600.046.039
CGATTAT206.313277E-446.014
CGATTAC206.313277E-446.022
GCGCATA850.046.033
Produced by FastQC (version 0.11.5)