##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527993_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3681942
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92235510499622	33.0	31.0	34.0	30.0	34.0
2	32.406087874279386	34.0	31.0	34.0	30.0	34.0
3	32.52343165644652	34.0	31.0	34.0	31.0	34.0
4	36.10627408036303	37.0	35.0	37.0	35.0	37.0
5	36.073798012027346	37.0	35.0	37.0	35.0	37.0
6	36.194461238118365	37.0	37.0	37.0	35.0	37.0
7	36.32597634617819	37.0	37.0	37.0	35.0	37.0
8	36.22263332773846	37.0	37.0	37.0	35.0	37.0
9	37.806816076950696	39.0	38.0	39.0	35.0	39.0
10	37.17501552170023	39.0	37.0	39.0	33.0	39.0
11	38.122346576887956	39.0	38.0	39.0	37.0	39.0
12	38.137911732449886	39.0	39.0	39.0	37.0	39.0
13	38.105874834530255	39.0	39.0	39.0	37.0	39.0
14	38.73818408872275	40.0	39.0	40.0	36.0	40.0
15	38.138783554982666	40.0	38.0	40.0	34.0	40.0
16	37.59268559906701	40.0	37.0	40.0	33.0	40.0
17	37.615584384544896	40.0	37.0	40.0	33.0	40.0
18	37.663956412132514	40.0	37.0	40.0	33.0	40.0
19	37.79163387147326	40.0	37.0	40.0	33.0	40.0
20	37.81044568328344	40.0	37.0	40.0	33.0	40.0
21	37.620388914328366	40.0	37.0	40.0	33.0	40.0
22	37.69175532911708	40.0	37.0	40.0	33.0	40.0
23	37.64412883201311	40.0	37.0	40.0	33.0	40.0
24	37.6101823439913	40.0	37.0	40.0	33.0	40.0
25	37.68273617563775	40.0	37.0	40.0	33.0	40.0
26	37.68453957178033	40.0	37.0	40.0	33.0	40.0
27	37.63807876386972	40.0	37.0	40.0	33.0	40.0
28	37.603096952640755	40.0	37.0	40.0	33.0	40.0
29	37.62114666662321	40.0	37.0	40.0	33.0	40.0
30	37.56430791142283	40.0	37.0	40.0	33.0	40.0
31	37.54511939623166	40.0	37.0	40.0	33.0	40.0
32	37.4747999832697	40.0	36.0	40.0	33.0	40.0
33	37.4161203517057	40.0	36.0	40.0	33.0	40.0
34	37.33812645609301	40.0	36.0	40.0	33.0	40.0
35	37.26428661831175	40.0	36.0	40.0	33.0	40.0
36	37.18722809865012	40.0	35.0	40.0	33.0	40.0
37	37.04724843574397	39.0	35.0	40.0	33.0	40.0
38	36.84261050282704	39.0	35.0	40.0	32.0	40.0
39	36.73284234243777	39.0	35.0	40.0	32.0	40.0
40	36.58711815666841	39.0	35.0	40.0	31.0	40.0
41	36.42617075445512	39.0	35.0	40.0	31.0	40.0
42	36.270238640369676	38.0	35.0	40.0	31.0	40.0
43	36.109843935618755	38.0	35.0	40.0	31.0	40.0
44	35.96602825356836	38.0	35.0	40.0	31.0	40.0
45	35.801169056981344	37.0	35.0	40.0	30.0	40.0
46	35.62400222491283	37.0	35.0	40.0	30.0	40.0
47	35.47897223802004	37.0	35.0	40.0	30.0	40.0
48	35.315289051266966	36.0	34.0	40.0	30.0	40.0
49	35.164439037877294	36.0	34.0	40.0	30.0	40.0
50	34.992067501334894	36.0	34.0	39.0	29.0	40.0
51	34.806152568400044	35.0	34.0	39.0	29.0	40.0
52	34.17696965351437	35.0	33.0	38.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	5.0
14	10.0
15	22.0
16	65.0
17	212.0
18	503.0
19	992.0
20	1726.0
21	2679.0
22	4360.0
23	6735.0
24	10329.0
25	14693.0
26	20807.0
27	27606.0
28	37227.0
29	48018.0
30	61399.0
31	76996.0
32	95813.0
33	123535.0
34	181852.0
35	330397.0
36	329749.0
37	640280.0
38	1134177.0
39	531750.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.845429938874652	18.629761142353683	35.357564024637	17.16724489413467
2	22.581262822716923	20.93422438484908	35.90787144392823	20.57664134850576
3	27.14716853225825	21.825601815563633	34.21362965521999	16.813599996958125
4	33.41239487205393	26.81603892728348	31.776926415462274	7.9946397852003095
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	100.0	0.0
8	0.0	100.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	100.0	0.0	0.0
11	99.47541270340488	0.3622001650216109	0.06613357842138741	0.09625355315211374
12	99.5215025114464	0.36803947482062455	0.039245593765463986	0.07121241996750628
13	98.62048342966835	0.777171394878029	0.12908948592889297	0.4732556895247128
14	65.86051599943725	23.50058746172536	3.9193447370979775	6.719551801739408
15	33.869300494141406	25.42813004658954	17.12107360735177	23.581495851917275
16	28.785733181022405	15.052083927449155	30.00033677879771	26.16184611273073
17	21.53352768729111	16.293276754495317	31.83868730142952	30.334508256784055
18	23.48673607569049	16.80409414379694	34.45029823935304	25.25887154115953
19	24.42670199584893	17.14671225130651	30.26943390200063	28.15715185084393
20	26.988094869500934	17.788085743881897	27.012864406880933	28.210954979736236
21	30.27209554088576	18.847255062681594	21.9101224299568	28.970526966475845
22	30.077225551081465	18.960075959914633	23.12711607081263	27.83558241819127
23	29.737024646232886	20.167563747609275	20.879090436514208	29.216321169643628
24	29.824043942028418	20.370690249873572	21.479290005111434	28.325975802986576
25	30.933349846358254	19.274366625003868	22.74430178422148	27.0479817444164
26	31.32868469954171	17.740284882271368	22.597232656027717	28.333797762159207
27	30.993046604210495	21.1724953842293	21.35085234911359	26.483605662446614
28	31.177324357635182	20.3403258389187	20.270742993778825	28.211606809667288
29	30.916619544794564	20.14287568897066	19.879183322279385	29.06132144395539
30	30.164000410652857	20.31916852573995	19.771522745333847	29.745308318273345
31	28.978457563970316	19.13976374424149	20.686827766434128	31.194950925354064
32	30.127009062065618	20.37921835813818	20.564582494781288	28.929190085014916
33	31.560328761289558	19.946647720143336	20.562029494218	27.93099402434911
34	28.561802440125344	20.54464736272326	22.084704213157078	28.808845983994313
35	31.312959302455063	21.567612960769072	19.661553604049168	27.457874132726694
36	33.53409695209756	20.94427342961948	20.10278814821092	25.418841470072046
37	32.51479789741392	20.123402269780456	20.406812491886075	26.954987340919544
38	30.581932034779474	23.481141202115623	21.85153921490344	24.085387548201464
39	31.553158632047978	23.397353896394893	22.7011452108697	22.34834226068743
40	32.59918271390478	22.488322738380994	20.394889436063902	24.51760511165032
41	32.25466343576298	23.455203802775817	18.65702936113605	25.63310340032515
42	32.20702553163521	23.20267402365382	19.862290063232933	24.728010381478036
43	32.401107893606145	22.280932181984397	19.431403319226646	25.886556605182808
44	30.09028930928298	24.427516783262746	20.447850617961937	25.034343289492337
45	29.704161553875647	22.41355241337316	21.11863793617607	26.76364809657512
46	27.38753081933393	22.973012611279593	21.30639211589971	28.333064453486774
47	29.518743098071614	20.524793709406612	23.071520409609928	26.884942782911843
48	29.703781319749194	20.01973415116262	22.02669134929339	28.249793179794793
49	29.494000720272073	19.86810221345149	23.605423442303003	27.032473623973434
50	30.084694435708116	20.911817730969144	21.167742457648707	27.83574537567403
51	27.838298376237326	22.012432569551613	22.678059567478247	27.471209486732818
52	29.71350444955407	21.618618652873945	22.249318430328344	26.418558467243646
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	1.0
8	1.0
9	5.5
10	10.0
11	53.5
12	97.0
13	233.5
14	572.5
15	775.0
16	1176.5
17	1578.0
18	2025.5
19	2473.0
20	2431.0
21	2389.0
22	2967.5
23	3546.0
24	4820.5
25	6095.0
26	7594.5
27	9094.0
28	12370.5
29	15647.0
30	17160.0
31	18673.0
32	19741.5
33	20810.0
34	21600.5
35	22391.0
36	26688.5
37	30986.0
38	34820.5
39	40664.0
40	42673.0
41	51415.0
42	60157.0
43	67873.5
44	75590.0
45	85279.0
46	94968.0
47	109291.5
48	123615.0
49	141274.5
50	158934.0
51	169729.5
52	180525.0
53	200509.5
54	220494.0
55	240250.5
56	260007.0
57	300797.0
58	341587.0
59	360139.0
60	378691.0
61	375717.0
62	372743.0
63	345845.0
64	287371.5
65	255796.0
66	229885.0
67	203974.0
68	179298.0
69	154622.0
70	130046.0
71	105470.0
72	89720.5
73	73971.0
74	59100.0
75	44229.0
76	33158.0
77	22087.0
78	16872.5
79	11658.0
80	8523.0
81	5388.0
82	3083.0
83	778.0
84	861.5
85	945.0
86	529.5
87	114.0
88	141.5
89	194.0
90	219.0
91	109.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3681942.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.4842788772839315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0526467550614	4.672094554062454
2	8.7744656999505	1.1379216519528281
3	4.3472186995577715	0.8456573516542856
4	2.605091097533726	0.6756854870855342
5	1.6959129476673847	0.5498386252135976
6	1.0433643475567265	0.4059279240103928
7	0.7234142715063079	0.3283573916177867
8	0.508990423491913	0.2640348681430732
9	0.35850664179029496	0.2092191340254126
>10	2.5462856049262674	3.5629212654285016
>50	0.969164154404604	4.706359553902127
>100	3.8985776806131316	60.53622727129769
>500	0.4304117443669991	18.343985758599832
>1k	0.04594993157297052	3.761769163006491
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.025937399339804917	0.0	0.0	0.0	0.0
9	0.025937399339804917	0.0	0.0	0.0	0.0
10	0.030065655569805283	0.0	0.0	0.0	0.0
11	0.030255772633028984	0.0	0.0	0.0	0.0
12	0.03332480522506873	0.0	0.0	0.0	0.0
13	0.03851228509302971	0.0	0.0	0.0	0.0
14	0.05111433042671503	0.0	0.0	0.0	0.0
15	0.07428145255954602	0.0	0.0	0.0	0.0
16	0.08639462544494182	0.0	0.0	0.0	0.0
17	0.0972584576291533	0.0	0.0	0.0	0.0
18	0.10874696016395695	0.0	0.0	0.0	0.0
19	0.1204255797619843	0.0	0.0	0.0	0.0
20	0.1303388266300773	0.0	0.0	0.0	0.0
21	0.14193596748672305	0.0	0.0	0.0	2.715958046052871E-5
22	0.1543750553376452	0.0	0.0	0.0	2.715958046052871E-5
23	0.1670585794127121	0.0	0.0	0.0	2.715958046052871E-5
24	0.17922607145902897	0.0	0.0	0.0	2.715958046052871E-5
25	0.19226267008008274	0.0	0.0	0.0	2.715958046052871E-5
26	0.2093460461897553	0.0	0.0	0.0	2.715958046052871E-5
27	0.22613066691436204	0.0	0.0	0.0	2.715958046052871E-5
28	0.24079684036304755	0.0	0.0	0.0	2.715958046052871E-5
29	0.26010730207048344	0.0	0.0	0.0	2.715958046052871E-5
30	0.28604470141028837	0.0	0.0	0.0	2.715958046052871E-5
31	0.3076908870373298	0.0	0.0	0.0	2.715958046052871E-5
32	0.3279519340608842	0.0	0.0	0.0	2.715958046052871E-5
33	0.34221071380266177	0.0	0.0	0.0	2.715958046052871E-5
34	0.3545683229122023	0.0	0.0	0.0	2.715958046052871E-5
35	0.36793083649878244	0.0	0.0	0.0	2.715958046052871E-5
36	0.3818093821141126	0.0	0.0	0.0	2.715958046052871E-5
37	0.3950632573788506	0.0	0.0	0.0	2.715958046052871E-5
38	0.4123367505517469	0.0	0.0	0.0	2.715958046052871E-5
39	0.42912137127635364	0.0	0.0	0.0	2.715958046052871E-5
40	0.4511477910298424	0.0	0.0	0.0	2.715958046052871E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	35	1.0203985E-7	46.000004	22
TCGTTAA	35	1.0203985E-7	46.000004	33
GTCGATA	70	0.0	46.000004	36
ATAGCCG	55	1.8189894E-12	46.000004	42
TAACGGG	55	1.8189894E-12	46.000004	35
TATGCGT	35	1.0203985E-7	46.000004	45
GCCGATA	35	1.0203985E-7	46.000004	15
CGAATCG	35	1.0203985E-7	46.000004	34
TTACGAC	55	1.8189894E-12	46.000004	34
ACCGTAC	70	0.0	46.000004	43
CGATAGG	70	0.0	46.000004	38
GATACGC	30	1.8625287E-6	46.0	13
ACGTTAG	50	1.6370905E-11	46.0	29
ATAATAC	25	3.418634E-5	46.0	31
TTCGTAA	30	1.8625287E-6	46.0	34
CACGATT	20	6.313277E-4	46.0	30
TAAGTCG	60	0.0	46.0	39
CGATTAT	20	6.313277E-4	46.0	14
CGATTAC	20	6.313277E-4	46.0	22
GCGCATA	85	0.0	46.0	33
>>END_MODULE