FastQCFastQC Report
Fri 17 Jun 2016
SRR1527992_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527992_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3650550
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA405.6170393E-946.00000436
CCCTATA1050.046.00000445
ATAATAC206.313267E-446.00000431
ATAGCCG750.046.00000442
ATATCGT206.313267E-446.00000430
ATATCGA405.6170393E-946.00000430
TTGACGT405.6170393E-946.00000439
CGGGATA206.313267E-446.00000437
TTAGTCG405.6170393E-946.00000420
TATAGCG206.313267E-446.00000424
AGTACGG206.313267E-446.00000429
TCGGTAC206.313267E-446.00000446
GCCGTAA405.6170393E-946.00000431
ACGGTTA206.313267E-446.00000438
TCGTTAA501.6370905E-1146.033
ACGTTGA301.8625251E-646.024
ATTACCG253.418628E-546.016
ACCGATT453.110472E-1046.042
CAATTCG501.6370905E-1146.025
TAACGGG301.8625251E-646.035
Produced by FastQC (version 0.11.5)