##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527992_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3650550
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87527358891126	33.0	31.0	34.0	30.0	34.0
2	32.246340688389424	34.0	31.0	34.0	30.0	34.0
3	32.45687581323362	34.0	31.0	34.0	30.0	34.0
4	36.076599964388926	37.0	35.0	37.0	35.0	37.0
5	36.05493500979304	37.0	35.0	37.0	35.0	37.0
6	36.18354823245812	37.0	37.0	37.0	35.0	37.0
7	36.3296481352125	37.0	37.0	37.0	35.0	37.0
8	36.17022914355371	37.0	37.0	37.0	35.0	37.0
9	37.79872512361151	39.0	38.0	39.0	35.0	39.0
10	37.16092369642931	39.0	37.0	39.0	33.0	39.0
11	38.11174836668447	39.0	38.0	39.0	37.0	39.0
12	38.108273273890234	39.0	39.0	39.0	37.0	39.0
13	38.10848255742285	39.0	39.0	39.0	37.0	39.0
14	38.765849255591625	40.0	39.0	40.0	36.0	40.0
15	38.167317527495854	40.0	38.0	40.0	34.0	40.0
16	37.630656750352685	40.0	37.0	40.0	33.0	40.0
17	37.667831696593666	40.0	37.0	40.0	33.0	40.0
18	37.73673282108175	40.0	37.0	40.0	33.0	40.0
19	37.85207050992316	40.0	38.0	40.0	33.0	40.0
20	37.84745723247182	40.0	37.0	40.0	33.0	40.0
21	37.84371889167386	40.0	37.0	40.0	33.0	40.0
22	37.81287230691266	40.0	37.0	40.0	33.0	40.0
23	37.741236799934256	40.0	37.0	40.0	33.0	40.0
24	37.70592184739286	40.0	37.0	40.0	33.0	40.0
25	37.63441645779403	40.0	37.0	40.0	33.0	40.0
26	37.62098040021367	40.0	37.0	40.0	33.0	40.0
27	37.60002574954459	40.0	37.0	40.0	33.0	40.0
28	37.425976907589266	40.0	37.0	40.0	33.0	40.0
29	37.54545945131556	40.0	37.0	40.0	33.0	40.0
30	37.551660708660336	40.0	37.0	40.0	33.0	40.0
31	37.57043513991042	40.0	37.0	40.0	33.0	40.0
32	37.49520784539316	40.0	36.0	40.0	33.0	40.0
33	37.43138513374697	40.0	36.0	40.0	33.0	40.0
34	37.37719576502171	40.0	36.0	40.0	33.0	40.0
35	37.23334894741888	40.0	35.0	40.0	33.0	40.0
36	36.781704400706744	39.0	35.0	40.0	31.0	40.0
37	36.85853501527167	39.0	35.0	40.0	32.0	40.0
38	36.773052005862134	39.0	35.0	40.0	32.0	40.0
39	36.704290586350005	39.0	35.0	40.0	32.0	40.0
40	36.56336113736286	39.0	35.0	40.0	31.0	40.0
41	36.42881346646396	39.0	35.0	40.0	31.0	40.0
42	36.128875648874825	38.0	35.0	40.0	31.0	40.0
43	35.97611264056101	38.0	35.0	40.0	30.0	40.0
44	35.834960485406306	38.0	35.0	40.0	30.0	40.0
45	35.43518757447508	37.0	35.0	40.0	29.0	40.0
46	35.4158882908055	37.0	35.0	40.0	29.0	40.0
47	35.34565613400721	37.0	35.0	40.0	29.0	40.0
48	35.181604963635614	36.0	34.0	40.0	29.0	40.0
49	35.03966361233239	36.0	34.0	40.0	29.0	40.0
50	34.80600019175193	36.0	34.0	39.0	28.0	40.0
51	34.710105052663295	35.0	34.0	39.0	28.0	40.0
52	34.21916067441892	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	5.0
14	15.0
15	27.0
16	66.0
17	189.0
18	495.0
19	981.0
20	1706.0
21	2790.0
22	4404.0
23	6951.0
24	10572.0
25	15188.0
26	21004.0
27	28076.0
28	37603.0
29	48875.0
30	62520.0
31	78460.0
32	97256.0
33	125637.0
34	184363.0
35	328590.0
36	334866.0
37	640967.0
38	1094990.0
39	523948.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.840010409390366	18.65094300858775	35.30755639561162	17.20149018641027
2	22.68107545438359	21.028831272000108	35.76798016737204	20.522113106244262
3	27.22151456629823	21.775540671953543	34.20643464683404	16.79651011491419
4	33.443289367355604	26.7989481037104	31.758255605319746	7.999506923614249
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	100.0	0.0
8	0.0	100.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	100.0	0.0	0.0
11	99.46797057977565	0.36328772376765145	0.07103039268055499	0.09771130377614333
12	99.51930531015874	0.36627357521469367	0.04473298544055006	0.06968812918601307
13	98.61020942049828	0.788675679007273	0.13392502499623343	0.4671898754982126
14	65.85574776403556	23.535741189683744	3.895933489474189	6.712577556806508
15	33.89360507320814	25.439125611209267	17.131035049513088	23.536234266069496
16	28.76550108887702	15.08978099190533	29.96638862637137	26.178329292846282
17	21.557025653668624	16.29477749928093	31.79764692991467	30.350549917135773
18	23.432660831929436	16.79505827888948	34.50669077262331	25.265590116557778
19	24.45056224404542	17.21318705400556	30.263631507581053	28.07261919436797
20	26.990754817767186	17.797893468107546	26.99987673090356	28.211474983221706
21	29.938201092985988	18.946460122447302	21.914341674542193	29.200997110024517
22	30.097820876306308	18.979797564750516	23.096738847570915	27.82564271137226
23	29.7362041336237	20.180767281642492	20.895974579173	29.18705400556081
24	29.823779978359426	20.427058936324663	21.489802906411363	28.25935817890455
25	30.981112435112518	19.22397994822698	22.73356069633343	27.061346920327072
26	31.407815260714138	17.71428962759036	22.555806659270523	28.322088452424975
27	31.00064373861473	21.18516387941543	21.356343564668336	26.457848817301503
28	31.281094629576366	20.374162797386695	20.27053457698155	28.07420799605539
29	30.86940324060758	20.159318458862362	19.875607785128267	29.09567051540179
30	30.119324485351523	20.361178452561944	19.74360027940995	29.775896782676583
31	29.0250784128419	19.146429990001508	20.707811151744256	31.120680445412336
32	30.18142471682349	20.427524619577873	20.53200202709181	28.859048636506827
33	31.565051841503333	19.977044554930078	20.577803344701483	27.880100258865102
34	28.567613099395984	20.52613989672789	22.101847666789936	28.804399337086195
35	31.35018010984646	21.57329717439838	19.628220405144432	27.44830231061073
36	33.611373628631306	20.883154593143498	20.143759159578696	25.361712618646504
37	32.493925572858885	20.120118886195232	20.454260316938544	26.931695224007342
38	30.578022489761818	23.54497267535029	21.84090068619797	24.036104148689923
39	31.597375737902507	23.397405870348305	22.693155825834463	22.312062565914726
40	32.57580364602594	22.54449329553081	20.388379833175822	24.491323225267426
41	32.27239730999438	23.452493459889606	18.619797016887865	25.655312213228143
42	32.28077960855214	23.244250866307816	19.86941693717385	24.605552587966198
43	32.361178452561944	22.32055443700264	19.376340551423755	25.94192655901166
44	30.340441851227894	24.31671939844681	20.307926202900934	25.03491254742436
45	29.766802262672748	22.455575187300543	21.139280382408128	26.63834216761858
46	27.44370026434373	22.991658791141063	21.250195176069358	28.31444576844585
47	29.483694237854568	20.535371382394434	23.04800646477928	26.932927914971717
48	29.730588541452658	20.010135459040416	22.02306501760009	28.236210981906833
49	29.517853474134036	19.904041856706524	23.578501869581295	26.99960279957815
50	30.1815616824862	20.888468860856584	21.244552190765774	27.685417265891445
51	27.831093944748055	22.03572064483434	22.66146197148375	27.47172343893386
52	29.79090821930942	21.558724027886207	22.227335606963333	26.423032145841034
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	8.5
10	17.0
11	42.5
12	68.0
13	219.5
14	571.5
15	772.0
16	1156.5
17	1541.0
18	1986.5
19	2432.0
20	2393.0
21	2354.0
22	2949.5
23	3545.0
24	4926.5
25	6308.0
26	7535.0
27	8762.0
28	11958.5
29	15155.0
30	16806.5
31	18458.0
32	19537.5
33	20617.0
34	21399.5
35	22182.0
36	26575.5
37	30969.0
38	34719.0
39	40392.0
40	42315.0
41	50864.0
42	59413.0
43	67319.5
44	75226.0
45	84543.5
46	93861.0
47	108628.0
48	123395.0
49	140375.5
50	157356.0
51	168087.5
52	178819.0
53	199160.5
54	219502.0
55	239323.5
56	259145.0
57	298431.5
58	337718.0
59	356623.5
60	375529.0
61	371982.0
62	368435.0
63	341835.0
64	284595.5
65	253956.0
66	228125.5
67	202295.0
68	177681.5
69	153068.0
70	128758.5
71	104449.0
72	88796.5
73	73144.0
74	58644.5
75	44145.0
76	33155.0
77	22165.0
78	16959.5
79	11754.0
80	8540.5
81	5327.0
82	3049.0
83	771.0
84	870.5
85	970.0
86	561.0
87	152.0
88	170.5
89	192.0
90	195.0
91	98.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3650550.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.798996538396825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.59910018419214	4.868020343046498
2	9.188293406095168	1.2494235012363084
3	4.447892571012809	0.9072361858043112
4	2.7279241333343487	0.7418858695819758
5	1.6445753504441503	0.559073105740126
6	1.0498684678824155	0.4282831247342712
7	0.7485136341391777	0.35623991252185677
8	0.4767171146144164	0.25929584096463515
9	0.34772232847893036	0.21277466168863776
>10	2.5077537859087626	3.691578178998165
>50	1.0037355898658749	5.124401535188162
>100	3.835344179269804	61.32945512311833
>500	0.38521290553278775	17.07565143570792
>1k	0.03734634922921362	3.1966811816688154
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.024900357480379668	0.0	0.0	0.0	0.0
9	0.024900357480379668	0.0	0.0	0.0	0.0
10	0.0293380449521305	0.0	0.0	0.0	0.0
11	0.029447617482297187	0.0	0.0	0.0	0.0
12	0.03265261398967279	0.0	0.0	0.0	0.0
13	0.03744641218446535	0.0	0.0	0.0	0.0
14	0.0510060127925929	0.0	0.0	0.0	0.0
15	0.07283833942830532	0.0	0.0	0.0	0.0
16	0.0845078138910575	0.0	0.0	0.0	0.0
17	0.09614989522126803	0.0	0.0	0.0	0.0
18	0.10801112161181192	0.0	0.0	0.0	0.0
19	0.119105340291189	0.0	0.0	0.0	0.0
20	0.1285285778855241	0.0	0.0	0.0	0.0
21	0.1405815562038597	0.0	0.0	0.0	0.0
22	0.1536754735587788	0.0	0.0	0.0	0.0
23	0.16698853597403132	0.0	0.0	0.0	0.0
24	0.17928805248524196	0.0	0.0	0.0	0.0
25	0.19320376381641122	0.0	0.0	0.0	0.0
26	0.20835216611195573	0.0	0.0	0.0	0.0
27	0.22517154949254223	0.0	0.0	0.0	0.0
28	0.23977208913725329	0.0	0.0	0.0	0.0
29	0.25856377806084013	0.0	0.0	0.0	0.0
30	0.28428592951746995	0.0	0.0	0.0	0.0
31	0.3068030844667242	0.0	0.0	0.0	0.0
32	0.3262248154387695	0.0	0.0	0.0	0.0
33	0.34016791990248046	0.0	0.0	0.0	0.0
34	0.3539192724383997	0.0	0.0	0.0	0.0
35	0.36671186533536043	0.0	0.0	0.0	0.0
36	0.37953185136486284	0.0	0.0	0.0	0.0
37	0.3917217953459068	0.0	0.0	0.0	0.0
38	0.4056922929421594	0.0	0.0	0.0	0.0
39	0.4223473175274959	0.0	0.0	0.0	0.0
40	0.44538494199504186	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATA	40	5.6170393E-9	46.000004	36
CCCTATA	105	0.0	46.000004	45
ATAATAC	20	6.313267E-4	46.000004	31
ATAGCCG	75	0.0	46.000004	42
ATATCGT	20	6.313267E-4	46.000004	30
ATATCGA	40	5.6170393E-9	46.000004	30
TTGACGT	40	5.6170393E-9	46.000004	39
CGGGATA	20	6.313267E-4	46.000004	37
TTAGTCG	40	5.6170393E-9	46.000004	20
TATAGCG	20	6.313267E-4	46.000004	24
AGTACGG	20	6.313267E-4	46.000004	29
TCGGTAC	20	6.313267E-4	46.000004	46
GCCGTAA	40	5.6170393E-9	46.000004	31
ACGGTTA	20	6.313267E-4	46.000004	38
TCGTTAA	50	1.6370905E-11	46.0	33
ACGTTGA	30	1.8625251E-6	46.0	24
ATTACCG	25	3.418628E-5	46.0	16
ACCGATT	45	3.110472E-10	46.0	42
CAATTCG	50	1.6370905E-11	46.0	25
TAACGGG	30	1.8625251E-6	46.0	35
>>END_MODULE