FastQCFastQC Report
Fri 17 Jun 2016
SRR1527991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3716542
Sequences flagged as poor quality0
Sequence length59
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGC353.3447577E-853.013
ACGCATT150.00669743853.035
ACGTTAG353.3447577E-853.029
CAATTCG307.014751E-753.025
CGACATA203.1391587E-453.019
TAACGGG307.014751E-753.035
TATGCGT150.00669743853.045
ATCGTAC307.014751E-753.041
ACGATTA251.4793777E-553.049
CCGTATA203.1391587E-453.023
TCGGATA353.3447577E-853.035
ATATCGT203.1391587E-453.030
ATATCGC150.00669743853.019
CTAGTCG150.00669743853.027
CACTACG203.1391587E-453.041
ACGTCTA251.4793777E-553.019
CGTAACA150.00669743853.044
ATACGCC307.014751E-753.014
CGACTAA251.4793777E-553.018
CGATATC353.3447577E-853.044
Produced by FastQC (version 0.11.5)