##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527991_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3716542
Sequences flagged as poor quality	0
Sequence length	59
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52072141253886	31.0	31.0	34.0	30.0	34.0
2	31.993470543316878	33.0	31.0	34.0	30.0	34.0
3	32.18346355294788	34.0	31.0	34.0	30.0	34.0
4	35.89069624398164	37.0	35.0	37.0	35.0	37.0
5	35.763150799856426	37.0	35.0	37.0	33.0	37.0
6	35.937863745384824	37.0	35.0	37.0	35.0	37.0
7	36.099735991144456	37.0	35.0	37.0	35.0	37.0
8	35.96401977967692	37.0	35.0	37.0	35.0	37.0
9	37.57447648916654	39.0	37.0	39.0	35.0	39.0
10	36.75135919357295	38.0	37.0	39.0	32.0	39.0
11	37.7739350180894	39.0	37.0	39.0	35.0	39.0
12	37.8013537853198	39.0	37.0	39.0	35.0	39.0
13	37.81799371566365	39.0	38.0	39.0	35.0	39.0
14	38.24355328151814	40.0	38.0	40.0	35.0	40.0
15	37.62789738418132	40.0	37.0	40.0	33.0	40.0
16	37.07732940997303	39.0	36.0	40.0	32.0	40.0
17	37.035001622475946	39.0	36.0	40.0	32.0	40.0
18	37.04253685280565	39.0	36.0	40.0	32.0	40.0
19	37.16902405515665	40.0	36.0	40.0	32.0	40.0
20	37.28056564408528	40.0	36.0	40.0	32.0	40.0
21	37.2447912602629	40.0	36.0	40.0	32.0	40.0
22	37.21627308395815	40.0	36.0	40.0	32.0	40.0
23	37.16741691604723	40.0	36.0	40.0	32.0	40.0
24	37.11765210779267	39.0	36.0	40.0	32.0	40.0
25	37.01697142128355	39.0	36.0	40.0	31.0	40.0
26	36.87682178756489	39.0	35.0	40.0	31.0	40.0
27	36.72869134803266	39.0	35.0	40.0	31.0	40.0
28	36.713211905045064	39.0	35.0	40.0	31.0	40.0
29	36.69339536590734	39.0	35.0	40.0	31.0	40.0
30	36.641646724293714	39.0	35.0	40.0	30.0	40.0
31	36.650247730282615	39.0	35.0	40.0	31.0	40.0
32	36.504590288499365	39.0	35.0	40.0	30.0	40.0
33	36.38310881459163	39.0	35.0	40.0	30.0	40.0
34	36.31694946539014	39.0	35.0	40.0	30.0	40.0
35	36.190538678158354	39.0	35.0	40.0	30.0	40.0
36	36.015188581213394	38.0	35.0	40.0	30.0	40.0
37	35.854455835558966	38.0	35.0	40.0	29.0	40.0
38	35.62997512203548	38.0	34.0	40.0	28.0	40.0
39	35.50664972977569	38.0	34.0	40.0	28.0	40.0
40	35.32733196611259	38.0	34.0	40.0	28.0	40.0
41	35.16768275456056	37.0	34.0	40.0	27.0	40.0
42	35.030792602370695	37.0	34.0	40.0	27.0	40.0
43	34.85433798407229	37.0	33.0	40.0	27.0	40.0
44	34.70197107956805	37.0	33.0	40.0	26.0	40.0
45	34.48313378403903	36.0	33.0	40.0	26.0	40.0
46	34.25263403454071	36.0	33.0	39.0	26.0	40.0
47	34.14111746887295	36.0	33.0	39.0	25.0	40.0
48	33.94662538456447	35.0	33.0	39.0	25.0	40.0
49	33.81916415851079	35.0	33.0	39.0	24.0	40.0
50	33.599840389265076	35.0	33.0	39.0	24.0	40.0
51	33.40424163106458	35.0	32.0	38.0	24.0	40.0
52	33.24718138527696	35.0	32.0	38.0	24.0	40.0
53	32.999638373520334	35.0	32.0	38.0	23.0	40.0
54	32.823944139471585	35.0	32.0	37.0	23.0	40.0
55	32.687490414476684	35.0	31.0	37.0	23.0	40.0
56	32.47341130545545	35.0	31.0	37.0	23.0	39.0
57	32.32469241569179	35.0	31.0	36.0	23.0	39.0
58	32.135366693017325	35.0	31.0	36.0	22.0	39.0
59	31.652162951474786	34.0	30.0	36.0	21.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	8.0
14	23.0
15	115.0
16	396.0
17	1041.0
18	2008.0
19	3671.0
20	5975.0
21	9351.0
22	13944.0
23	19618.0
24	26103.0
25	33799.0
26	42792.0
27	53733.0
28	66581.0
29	80194.0
30	97703.0
31	119434.0
32	146761.0
33	186448.0
34	264750.0
35	369654.0
36	462856.0
37	699518.0
38	831396.0
39	178665.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.937759885398844	18.894149453981683	34.84787740862339	17.320213251996076
2	22.308586853047807	21.46874702344276	35.67127722490422	20.551388898605207
3	27.12612961188115	21.914537761176923	34.12257953764548	16.83675308929645
4	33.5171511582541	26.99894148915847	31.493576555841425	7.990330796746008
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	100.0	0.0
8	0.0	100.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	100.0	0.0	0.0
11	99.44634017320402	0.37798577279632517	0.0690157678831559	0.10665828611650292
12	99.50588477138157	0.37421883029977865	0.03960670967797485	0.08028968864067727
13	98.58231119142472	0.7993990112314081	0.13388789902011064	0.48440189832376446
14	65.48253726178798	23.79031368406438	3.989784051949366	6.737365002198279
15	33.89782760426224	25.507447514382992	17.132834769525005	23.46189011182976
16	28.8094416799272	15.173378909749976	29.819735657500978	26.19744375282184
17	21.543197951213788	16.38587697919195	31.75863477393771	30.312290295656553
18	23.337903890229143	16.991359172047567	34.36441724592377	25.30631969179953
19	24.408872548729438	17.30762090136476	30.208241962555515	28.075264587350286
20	26.958285416927886	17.92198231581938	27.00122856138852	28.118503705864217
21	29.920555182747833	19.02663820293165	21.98280014056077	29.070006473759747
22	30.021912842637054	19.125143749216342	23.12633625558382	27.72660715256279
23	29.696287570542722	20.341435667886977	20.90104188248108	29.06123487908922
24	29.817798372788467	20.490068456107853	21.476980483470925	28.21515268763275
25	30.88354712525783	19.39359221555952	22.806576651091255	26.91628400809139
26	31.281766760607038	17.81115348622456	22.638813176334345	28.268266576834055
27	30.906014246576525	21.256183839709063	21.446199181927717	26.3916027317867
28	31.18350337491141	20.434721308140738	20.316304780088586	28.06547053685926
29	30.767632923292677	20.277263111785096	19.95174546661924	29.003358498302994
30	30.106776675737823	20.47900978920728	19.776905521315243	29.637308013739656
31	29.016219916255487	19.23051051219117	20.726497911230386	31.026771660322954
32	30.046236528471898	20.587094132126047	20.57754224222409	28.789127097177968
33	31.53651970030206	20.084422562694034	20.54038404516887	27.83867369183504
34	28.584366865758547	20.650594019924974	22.07253409217493	28.69250502214155
35	31.319785973090035	21.60691309286966	19.660049583725947	27.41325135031435
36	33.48795735390586	21.05871533269367	20.17197706900662	25.281350244393845
37	32.45718735319014	20.195412832681562	20.531397196641393	26.8160026174869
38	30.527624872798427	23.595562756992923	21.91870830465524	23.95810406555341
39	31.475226164536817	23.590611918283177	22.637198772407256	22.29696314477275
40	32.52967408951655	22.705218991202038	20.33282551360916	24.432281405672263
41	32.22258755585165	23.592441576067216	18.636275333360956	25.548695534720178
42	32.00208688614309	23.42029230397504	19.895644930152816	24.68197587972906
43	32.309630834253994	22.459425993302375	19.424857838280854	25.806085334162777
44	30.01900691556829	24.533235464579707	20.433187624410003	25.014569995442
45	29.639702712898174	22.580371754173637	21.148933605485958	26.63099192744223
46	27.41946680543365	23.104918496817742	21.21622734251355	28.259387355235056
47	29.40747070798608	20.679545663684145	22.985237352356034	26.92774627597374
48	29.552793968156422	20.182336160872122	22.053161245049836	28.211708625921624
49	29.5073484976088	20.0388156517537	23.534430661620398	26.919405189017105
50	30.01217260561027	21.03778189510572	21.169920856538145	27.78012464274586
51	27.741325135031435	22.1232263754856	22.624122100597813	27.511326388885156
52	29.62339723323455	21.667964468045835	22.31178875416987	26.396849544549745
53	29.16848511331232	21.927856593575427	21.49385100450903	27.409807288603222
54	26.600398973023847	22.5016157492637	23.700606639182336	27.197378638530118
55	27.296260879064466	22.125540354447764	22.446860549403183	28.13133821708459
56	28.133840543171583	22.984107269607073	22.10966538249803	26.772386804723315
57	28.886852348231233	21.302624859345055	22.428052743652568	27.38247004877114
58	28.182918422555158	21.38043912863086	22.13926278782804	28.29737966098594
59	27.660201337695096	21.682009782211527	22.833483383209447	27.824305496883934
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	0.5
7	0.0
8	7.0
9	31.5
10	49.0
11	163.0
12	277.0
13	408.5
14	812.0
15	1084.0
16	1407.0
17	1730.0
18	1555.5
19	1527.0
20	1673.0
21	1972.0
22	2271.0
23	2598.5
24	2926.0
25	3847.0
26	7148.5
27	9529.0
28	11016.0
29	12503.0
30	15073.5
31	18364.5
32	19085.0
33	21352.5
34	23620.0
35	23986.0
36	29665.0
37	34978.0
38	36502.0
39	38026.0
40	40983.0
41	43940.0
42	54372.0
43	68269.5
44	71735.0
45	81230.0
46	90725.0
47	95896.5
48	111486.5
49	121905.0
50	136825.0
51	151745.0
52	158866.0
53	192919.0
54	219851.0
55	236119.5
56	252388.0
57	285976.0
58	325209.5
59	330855.0
60	325065.0
61	319275.0
62	305063.0
63	290851.0
64	270342.0
65	225152.0
66	200471.0
67	179604.0
68	158737.0
69	133177.5
70	101805.0
71	95992.0
72	86399.5
73	76807.0
74	58080.0
75	30740.5
76	22128.0
77	17170.5
78	12213.0
79	8445.5
80	4678.0
81	4564.0
82	3102.5
83	1755.0
84	1132.5
85	510.0
86	408.0
87	231.5
88	157.0
89	133.0
90	109.0
91	194.5
92	140.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	3716542.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.344290770473876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.72274350381683	9.157422740976791
2	7.332460161764937	1.6636312027595468
3	3.011556862277204	1.0249193015246563
4	1.7814831127515824	0.8083864973497138
5	1.0791829384217932	0.6121282523995613
6	0.6823227672861477	0.46442807228450633
7	0.45669045629738203	0.36265805298365256
8	0.29982037964028585	0.2720999652442614
9	0.22732313914955232	0.23209378104324732
>10	1.4337772508492868	3.499413135707077
>50	0.5619814771666466	4.781820271677632
>100	2.1640245426636997	57.303480622401736
>500	0.22436295609530493	16.62661669139235
>1k	0.022270451819386125	3.1909014122552612
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.025642115708634533	0.0	0.0	0.0	0.0
9	0.025642115708634533	0.0	0.0	0.0	0.0
10	0.029086177419762778	0.0	0.0	0.0	0.0
11	0.029220711080353727	0.0	0.0	0.0	0.0
12	0.03191138429217267	0.0	0.0	0.0	0.0
13	0.03670078260921039	0.0	0.0	0.0	0.0
14	0.04856665147333193	0.0	0.0	0.0	0.0
15	0.06767043127724642	0.0	0.0	0.0	0.0
16	0.07872909817782228	0.0	0.0	0.0	0.0
17	0.08828098807977953	0.0	0.0	0.0	0.0
18	0.09909749439129169	0.0	0.0	0.0	0.0
19	0.11039832188093125	0.0	0.0	0.0	0.0
20	0.11965423772958841	0.0	0.0	0.0	0.0
21	0.13122413254040988	0.0	0.0	0.0	0.0
22	0.14357432258265881	0.0	0.0	0.0	0.0
23	0.15603213955338052	0.0	0.0	0.0	0.0
24	0.16744059397149286	0.0	0.0	0.0	0.0
25	0.1800329446028055	0.0	0.0	0.0	0.0
26	0.19563884923135538	0.0	0.0	0.0	0.0
27	0.21102950000295975	0.0	0.0	0.0	0.0
28	0.22545150841830927	0.0	0.0	0.0	0.0
29	0.24283325736665964	0.0	0.0	0.0	0.0
30	0.26465461711451127	0.0	0.0	0.0	0.0
31	0.2872831788259086	0.0	0.0	0.0	0.0
32	0.3059295441838139	0.0	0.0	0.0	0.0
33	0.31900621599325396	0.0	0.0	0.0	0.0
34	0.33197526087422125	0.0	0.0	0.0	0.0
35	0.3448904922909522	0.0	0.0	0.0	0.0
36	0.3571868688689648	0.0	0.0	0.0	0.0
37	0.3687836704119044	0.0	0.0	0.0	0.0
38	0.3828020778454811	0.0	0.0	0.0	0.0
39	0.3983810757419128	0.0	0.0	0.0	0.0
40	0.4183189642414912	0.0	0.0	0.0	0.0
41	0.43416702945910474	0.0	0.0	0.0	0.0
42	0.4485083176780997	0.0	0.0	0.0	0.0
43	0.4612082952378851	0.0	0.0	0.0	0.0
44	0.4745271276363889	0.0	0.0	0.0	0.0
45	0.48754998598159255	0.0	0.0	0.0	0.0
46	0.5031558906101424	0.0	0.0	0.0	0.0
47	0.5144298113676639	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGC	35	3.3447577E-8	53.0	13
ACGCATT	15	0.006697438	53.0	35
ACGTTAG	35	3.3447577E-8	53.0	29
CAATTCG	30	7.014751E-7	53.0	25
CGACATA	20	3.1391587E-4	53.0	19
TAACGGG	30	7.014751E-7	53.0	35
TATGCGT	15	0.006697438	53.0	45
ATCGTAC	30	7.014751E-7	53.0	41
ACGATTA	25	1.4793777E-5	53.0	49
CCGTATA	20	3.1391587E-4	53.0	23
TCGGATA	35	3.3447577E-8	53.0	35
ATATCGT	20	3.1391587E-4	53.0	30
ATATCGC	15	0.006697438	53.0	19
CTAGTCG	15	0.006697438	53.0	27
CACTACG	20	3.1391587E-4	53.0	41
ACGTCTA	25	1.4793777E-5	53.0	19
CGTAACA	15	0.006697438	53.0	44
ATACGCC	30	7.014751E-7	53.0	14
CGACTAA	25	1.4793777E-5	53.0	18
CGATATC	35	3.3447577E-8	53.0	44
>>END_MODULE