FastQCFastQC Report
Fri 17 Jun 2016
SRR1527990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10208558
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAT30400.043.5789451
ACTCGAT31950.043.408451
TACCGAT38400.043.1848951
CCGATCT406000.043.139163
AGATCTT2262150.042.8267824
TTGGGGC1615550.042.8181089
CTCGATC173400.042.7768172
CATATGC71000.042.76056337
ATCGATC187800.042.619812
TTAGATC307850.042.5782052
TAGATCT841950.042.5334053
TTCGATC316050.042.4777682
CGAATAT650.042.4615446
GCATATG71550.042.43186636
CGTAGAT32400.042.3796271
CGATCTT2515150.042.313824
TTGCGAT69800.042.3094561
TGCCGAT56200.042.2758031
GATCTTG11056750.042.261715
TTGGGAC393500.042.253379