FastQCFastQC Report
Fri 17 Jun 2016
SRR1527989_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527989_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10113588
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGAT33150.043.224741
CCGATCT395050.043.0831573
ATCGATC188550.042.9626122
TACCGAT35300.042.9376751
AGATCTT2235200.042.9253774
AATCGAT46150.042.860241
TTGGGGC1591300.042.79139
TTCGATC306000.042.7830052
CGAATAT700.042.71428346
TTGGGAC388000.042.6981969
TTAGATC314450.042.6134532
TAGATCT841050.042.5515753
ATGCGAT36500.042.471231
TGCCGAT55900.042.4615361
CTCGATC176100.042.4474752
CGGATCT513750.042.4319233
CATATGC68800.042.38953437
TATCGAT59650.042.3755231
ACACGAT29100.042.364261
CGATCTT2474950.042.3608174