##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527989_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10113588
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.736400573169483	31.0	31.0	34.0	30.0	34.0
2	32.20283276320926	34.0	31.0	34.0	30.0	34.0
3	32.416415222767625	34.0	31.0	34.0	30.0	34.0
4	35.98587860213408	37.0	35.0	37.0	35.0	37.0
5	35.96395631303154	37.0	35.0	37.0	35.0	37.0
6	35.726448318836006	37.0	35.0	37.0	33.0	37.0
7	36.1310150265168	37.0	35.0	37.0	35.0	37.0
8	35.94015496775229	37.0	35.0	37.0	35.0	37.0
9	38.101086281149676	39.0	39.0	39.0	35.0	39.0
10	38.199400549043524	39.0	39.0	39.0	37.0	39.0
11	38.115782252549735	39.0	38.0	39.0	37.0	39.0
12	38.08650510580419	39.0	39.0	39.0	37.0	39.0
13	38.10917164116236	39.0	39.0	39.0	37.0	39.0
14	38.7094387273834	40.0	39.0	40.0	36.0	40.0
15	38.249663324232706	40.0	38.0	40.0	34.0	40.0
16	37.59104919045546	40.0	36.0	40.0	33.0	40.0
17	37.56556792703044	40.0	36.0	40.0	33.0	40.0
18	37.53669963617264	40.0	36.0	40.0	33.0	40.0
19	37.65361432559839	40.0	37.0	40.0	33.0	40.0
20	37.61332180033436	40.0	36.0	40.0	33.0	40.0
21	37.54772381473321	40.0	36.0	40.0	33.0	40.0
22	37.50737354537282	40.0	36.0	40.0	33.0	40.0
23	37.46588411550876	40.0	36.0	40.0	33.0	40.0
24	37.46931731844327	40.0	36.0	40.0	33.0	40.0
25	37.44308686491876	40.0	36.0	40.0	33.0	40.0
26	37.47872545332082	40.0	36.0	40.0	33.0	40.0
27	37.53243112137849	40.0	36.0	40.0	33.0	40.0
28	37.40119609380964	40.0	36.0	40.0	33.0	40.0
29	37.493634207760884	40.0	36.0	40.0	33.0	40.0
30	37.51641899986434	40.0	36.0	40.0	33.0	40.0
31	37.52781426334551	40.0	36.0	40.0	33.0	40.0
32	37.4816982855145	40.0	35.0	40.0	33.0	40.0
33	37.411309517453155	40.0	35.0	40.0	33.0	40.0
34	37.338461978083345	40.0	35.0	40.0	33.0	40.0
35	37.18065141668812	40.0	35.0	40.0	33.0	40.0
36	36.7618259711588	40.0	35.0	40.0	32.0	40.0
37	36.86682886429623	39.0	35.0	40.0	32.0	40.0
38	36.746347784782216	39.0	35.0	40.0	31.0	40.0
39	36.68730484176338	39.0	35.0	40.0	31.0	40.0
40	36.645534206060205	39.0	35.0	40.0	31.0	40.0
41	36.557263851365114	39.0	35.0	40.0	31.0	40.0
42	36.28076029990543	39.0	35.0	40.0	31.0	40.0
43	36.14998514869303	39.0	35.0	40.0	31.0	40.0
44	36.017310078282804	38.0	35.0	40.0	30.0	40.0
45	35.61935269658997	38.0	35.0	40.0	29.0	40.0
46	35.6365811025721	38.0	35.0	40.0	29.0	40.0
47	35.583194213566934	38.0	35.0	40.0	30.0	40.0
48	35.433656779374445	37.0	35.0	40.0	29.0	40.0
49	35.300661249004804	37.0	35.0	40.0	29.0	40.0
50	35.07926563747703	37.0	34.0	40.0	28.0	40.0
51	34.989958855353805	37.0	34.0	40.0	28.0	40.0
52	34.463278709791226	36.0	34.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	5.0
13	3.0
14	8.0
15	61.0
16	110.0
17	375.0
18	1003.0
19	2138.0
20	4307.0
21	7619.0
22	12190.0
23	19062.0
24	28628.0
25	41083.0
26	58405.0
27	79205.0
28	106238.0
29	140351.0
30	181854.0
31	228311.0
32	281589.0
33	359865.0
34	544519.0
35	1056320.0
36	777102.0
37	1433985.0
38	2990436.0
39	1758815.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.92390910130015	20.690500740192302	34.47006146582202	15.915528692685523
2	26.81115742504045	20.97723379674948	34.1004102599394	18.111198518270665
3	19.757182119738317	22.397352947341734	35.62315372150813	22.222311211411817
4	22.343237632381307	20.53343482056022	34.44345369813364	22.679873848924835
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.48856923971987	0.320351194847961	0.13809144687325606	0.052988118558913014
12	99.06571238614822	0.6673002696965705	0.14953150157985476	0.11745584257535505
13	96.55805634953688	1.902915167198822	0.5057255644584295	1.0333029188058678
14	53.546496060547454	25.084282650232538	7.35202976431312	14.017191524906888
15	34.419159649374684	23.057267114302064	19.746345213983403	22.777228022339845
16	31.710694562602313	16.54644227152619	27.114610561553427	24.62825260431807
17	24.428887156566	16.620540603394165	29.260644194721003	29.68992804531883
18	22.370468324396843	19.72536354061486	33.011785728269736	24.892382406718568
19	27.17997806515353	19.173244945315155	29.801747905886618	23.845029083644697
20	31.175315822633863	18.820284156325133	25.882159724125604	24.122240296915397
21	31.561192724085657	20.46528887670726	22.951478743251158	25.02203965595593
22	30.74926524592459	21.2625232508977	24.330919946511564	23.65729155666614
23	28.980970947204888	23.642509463505927	22.300572259815212	25.07594732947397
24	28.3420087905499	25.770972675572708	22.03525593488681	23.851762598990586
25	28.235221763038005	22.951458967875695	25.110119178277778	23.703200090808522
26	27.593144984747255	21.204858256041277	25.1661131539074	26.035883605304072
27	29.841773265828113	21.990771227777916	23.630782665855087	24.536672840538888
28	32.35641000997865	21.107039361302835	21.24800812530627	25.28854250341224
29	30.961118843282918	20.259526094992204	20.099681735107264	28.679673326617618
30	26.934170148121517	20.801173628983108	22.57414480400032	29.690511418895056
31	24.97603224493622	21.287637977738463	22.533773374988183	31.202556402337134
32	26.122776605098014	25.068314034544414	20.732503637680317	28.076405722677254
33	30.112448717507572	24.38354222062437	20.452098701271993	25.051910360596064
34	28.881599685492425	22.924940189376905	23.575451165303548	24.618008959827115
35	31.94029655944063	22.12670715872547	22.456214352413802	23.476781929420103
36	34.43855929270601	21.04038645829749	21.144820216129034	23.376234032867465
37	32.77505470857622	21.60987772094335	22.63362913339954	22.981438437080886
38	29.012819189391543	23.922360689401227	25.524611048027662	21.540209073179568
39	27.422532932921527	26.9778341771486	25.23395257944065	20.365680310489214
40	28.675728139212314	28.982780394059954	20.810922889087433	21.5305685776403
41	30.576042844537465	27.324743701246284	20.24903525830793	21.850178195908317
42	30.437733868534096	27.048076310800873	19.680819507379578	22.833370313285453
43	29.80208408726952	24.935641040548617	20.05890491089809	25.203369961283773
44	28.696521946513936	24.284576354108946	20.333723303737507	26.685178395639607
45	25.256941453418907	25.347967506685066	22.17712447847391	27.21796656142212
46	25.525421838421735	24.25243148129032	22.30925364964442	27.912893030643527
47	26.55369192417172	21.529589696554773	22.209832949493297	29.70688542978021
48	27.50964346184559	21.355961899970612	21.188227165275073	29.946167472908726
49	28.873195150919734	22.261436791769647	21.61823281707738	27.247135240233238
50	27.954984917321134	25.006496210840307	20.879177597505453	26.1593412743331
51	26.047373098449334	25.036287813978582	22.26570827287012	26.650630814701966
52	26.77191319242983	23.496112358937303	23.429281477552774	26.302692971080095
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	18.5
6	34.0
7	61.5
8	89.0
9	433.5
10	778.0
11	1708.5
12	2639.0
13	4199.5
14	7815.0
15	9870.0
16	10843.0
17	11816.0
18	13489.0
19	15162.0
20	18749.0
21	22336.0
22	27310.5
23	32285.0
24	40236.0
25	48187.0
26	55370.5
27	62554.0
28	69095.5
29	75637.0
30	81128.0
31	86619.0
32	89057.5
33	91496.0
34	102021.5
35	112547.0
36	123455.5
37	134364.0
38	151529.0
39	191393.0
40	214092.0
41	237135.0
42	260178.0
43	310562.0
44	360946.0
45	395899.0
46	430852.0
47	482875.0
48	534898.0
49	568498.5
50	602099.0
51	637402.5
52	672706.0
53	741522.5
54	810339.0
55	859232.5
56	908126.0
57	957659.5
58	1007193.0
59	999988.0
60	992783.0
61	886116.5
62	779450.0
63	662609.5
64	462255.0
65	378741.0
66	313666.0
67	248591.0
68	213952.0
69	179313.0
70	154031.0
71	128749.0
72	105168.5
73	81588.0
74	66184.5
75	50781.0
76	39229.5
77	27678.0
78	19489.5
79	11301.0
80	7507.5
81	3714.0
82	3040.5
83	2367.0
84	1349.0
85	331.0
86	216.5
87	102.0
88	64.5
89	15.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1.0113588E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.262873515055245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.50504100057603	5.990999829916543
2	9.067566290288141	1.4984830669205924
3	4.255608036387129	1.0549065280295842
4	2.62187897703557	0.866570174361094
5	1.568247578691278	0.6479115691508839
6	1.0897488973337268	0.5402674381103705
7	0.72151777056443	0.41732670539269756
8	0.5535920772320753	0.36594090504842663
9	0.40392648017811666	0.30038340735839225
>10	2.9021479405107544	5.015189268729649
>50	0.9633777198342216	5.911960100160125
>100	3.0058848220599717	57.225685995065476
>500	0.30651154414381887	16.529916022973964
>1k	0.03495086516481181	3.6344589887824084
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0018094468550627137	0.0	0.0	0.0	0.0
12	0.0024422588699480343	0.0	0.0	0.0	0.0
13	0.006199580208329626	0.0	0.0	0.0	0.0
14	0.03422128724247023	0.0	0.0	0.0	0.0
15	0.070350898217329	0.0	0.0	0.0	0.0
16	0.08906829109510887	0.0	0.0	0.0	0.0
17	0.10508634522189356	0.0	0.0	0.0	0.0
18	0.12436733630043066	0.0	0.0	0.0	0.0
19	0.14412293639013177	0.0	0.0	0.0	0.0
20	0.1663306830375135	0.0	0.0	0.0	0.0
21	0.18487998522383944	0.0	0.0	0.0	0.0
22	0.20448726999755182	0.0	0.0	0.0	0.0
23	0.22713996259289976	0.0	0.0	0.0	0.0
24	0.25065288402098246	0.0	0.0	0.0	0.0
25	0.2738592871293551	0.0	0.0	0.0	0.0
26	0.2990630031597095	0.0	0.0	0.0	0.0
27	0.326422235115767	0.0	0.0	0.0	0.0
28	0.35520529410531654	0.0	0.0	0.0	0.0
29	0.38516498793504345	0.0	0.0	0.0	0.0
30	0.4193566121143159	0.0	0.0	0.0	0.0
31	0.45170912637532795	0.0	0.0	0.0	0.0
32	0.4823807337218008	0.0	0.0	0.0	0.0
33	0.5074262467484338	0.0	0.0	0.0	0.0
34	0.5316016432545997	0.0	0.0	0.0	0.0
35	0.5536017484595971	0.0	0.0	0.0	0.0
36	0.5755326398504665	0.0	0.0	0.0	0.0
37	0.5986105030183155	0.0	0.0	0.0	0.0
38	0.6268596268703056	0.0	0.0	0.0	0.0
39	0.6631672162243508	0.0	0.0	0.0	0.0
40	0.7115476722998801	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCGAT	3315	0.0	43.22474	1
CCGATCT	39505	0.0	43.083157	3
ATCGATC	18855	0.0	42.962612	2
TACCGAT	3530	0.0	42.937675	1
AGATCTT	223520	0.0	42.925377	4
AATCGAT	4615	0.0	42.86024	1
TTGGGGC	159130	0.0	42.7913	9
TTCGATC	30600	0.0	42.783005	2
CGAATAT	70	0.0	42.714283	46
TTGGGAC	38800	0.0	42.698196	9
TTAGATC	31445	0.0	42.613453	2
TAGATCT	84105	0.0	42.551575	3
ATGCGAT	3650	0.0	42.47123	1
TGCCGAT	5590	0.0	42.461536	1
CTCGATC	17610	0.0	42.447475	2
CGGATCT	51375	0.0	42.431923	3
CATATGC	6880	0.0	42.389534	37
TATCGAT	5965	0.0	42.375523	1
ACACGAT	2910	0.0	42.36426	1
CGATCTT	247495	0.0	42.360817	4
>>END_MODULE