FastQCFastQC Report
Fri 17 Jun 2016
SRR1527988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10282621
Sequences flagged as poor quality0
Sequence length59
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGAT37200.049.7943571
CCGATCT410450.049.3198933
CTACGAT30100.049.3023221
AGATCTT2314850.049.261144
TTGGGGC1605600.049.1246839
TACCGAT35600.048.980341
TTCGATC313500.048.9172252
TAGATCT864800.048.817243
TTGGGAC399850.048.698769
TTAGATC317300.048.6571042
GATCTTG11156600.048.5962335
ATGCGAT39550.048.577751
CTGATCT984550.048.534663
CGATCTT2521650.048.518974
CGGATCT513650.048.5166973
CTCGATC183300.048.489362
CGCGATC103250.048.4828072
ATCTTGG11087500.048.4820376
TCTTGGG10722000.048.470147
CTTGGGG5811150.048.365478
Produced by FastQC (version 0.11.5)