##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527988_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10282621
Sequences flagged as poor quality	0
Sequence length	59
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.331325447082023	31.0	31.0	33.0	30.0	34.0
2	31.896510627008425	31.0	31.0	34.0	30.0	34.0
3	32.13067806350151	33.0	31.0	34.0	30.0	34.0
4	35.79728174363326	37.0	35.0	37.0	35.0	37.0
5	35.60003660545303	37.0	35.0	37.0	33.0	37.0
6	35.2673287287356	37.0	35.0	37.0	32.0	37.0
7	35.78726960762241	37.0	35.0	37.0	35.0	37.0
8	35.64540110930861	37.0	35.0	37.0	33.0	37.0
9	37.82728041809574	39.0	37.0	39.0	35.0	39.0
10	37.94670677835933	39.0	38.0	39.0	35.0	39.0
11	37.77238838230058	39.0	37.0	39.0	35.0	39.0
12	37.819019100285814	39.0	37.0	39.0	35.0	39.0
13	37.84615994307288	39.0	38.0	39.0	35.0	39.0
14	38.14254099222367	40.0	38.0	40.0	34.0	40.0
15	37.67755050001357	40.0	37.0	40.0	33.0	40.0
16	37.0211283679521	39.0	35.0	40.0	32.0	40.0
17	36.914875205455886	39.0	35.0	40.0	32.0	40.0
18	36.81079152873572	39.0	35.0	40.0	31.0	40.0
19	36.95590676735046	39.0	35.0	40.0	32.0	40.0
20	37.01870486133837	39.0	35.0	40.0	32.0	40.0
21	36.928569962852855	39.0	35.0	40.0	31.0	40.0
22	36.90441347590269	39.0	35.0	40.0	31.0	40.0
23	36.89475835003546	39.0	35.0	40.0	31.0	40.0
24	36.88207092335699	39.0	35.0	40.0	31.0	40.0
25	36.8655278649286	39.0	35.0	40.0	31.0	40.0
26	36.75582169176516	39.0	35.0	40.0	31.0	40.0
27	36.68140700702671	39.0	35.0	40.0	31.0	40.0
28	36.70566521901371	39.0	35.0	40.0	31.0	40.0
29	36.669313300568014	39.0	35.0	40.0	31.0	40.0
30	36.633980674771536	39.0	35.0	40.0	31.0	40.0
31	36.64623649942948	39.0	35.0	40.0	31.0	40.0
32	36.54788589407312	39.0	35.0	40.0	31.0	40.0
33	36.40572330731629	39.0	35.0	40.0	30.0	40.0
34	36.30617106280587	39.0	35.0	40.0	30.0	40.0
35	36.15119578947819	38.0	35.0	40.0	30.0	40.0
36	36.01546444238293	38.0	35.0	40.0	30.0	40.0
37	35.882484436604244	38.0	35.0	40.0	30.0	40.0
38	35.588647194134644	38.0	34.0	40.0	28.0	40.0
39	35.4689528088218	38.0	34.0	40.0	28.0	40.0
40	35.40286119657624	38.0	34.0	40.0	28.0	40.0
41	35.30933523660942	38.0	34.0	40.0	28.0	40.0
42	35.20846046936865	38.0	34.0	40.0	28.0	40.0
43	35.02334463168486	37.0	34.0	40.0	27.0	40.0
44	34.89751717971517	37.0	34.0	40.0	26.0	40.0
45	34.67784721424625	37.0	34.0	40.0	26.0	40.0
46	34.4968877098553	37.0	33.0	40.0	25.0	40.0
47	34.388436469651076	37.0	33.0	40.0	25.0	40.0
48	34.20858125569347	36.0	33.0	40.0	24.0	40.0
49	34.10425668708396	36.0	33.0	39.0	24.0	40.0
50	33.89640578992457	36.0	33.0	39.0	24.0	40.0
51	33.70986278692952	35.0	33.0	39.0	24.0	40.0
52	33.53200657692236	35.0	33.0	39.0	23.0	40.0
53	33.327518538318195	35.0	32.0	39.0	23.0	40.0
54	33.164435701753476	35.0	32.0	38.0	23.0	40.0
55	33.03795627593393	35.0	32.0	38.0	23.0	40.0
56	32.83256370141426	35.0	32.0	38.0	22.0	40.0
57	32.66701933291133	35.0	32.0	38.0	22.0	40.0
58	32.472995941404434	35.0	31.0	37.0	21.0	40.0
59	31.866980121118925	34.0	31.0	37.0	20.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	11.0
14	35.0
15	206.0
16	735.0
17	2202.0
18	4885.0
19	9469.0
20	16185.0
21	25360.0
22	37233.0
23	52751.0
24	72193.0
25	95501.0
26	123594.0
27	157612.0
28	193914.0
29	228852.0
30	270651.0
31	328159.0
32	406040.0
33	516216.0
34	748630.0
35	1094288.0
36	1055141.0
37	1634340.0
38	2560017.0
39	648395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.97124186527929	21.01027549298958	33.9967309891126	16.02175165261853
2	26.227126332867854	21.46472188365204	34.049519086621984	18.258632696858125
3	19.79327060678401	22.567086737904667	35.47298884204718	22.166653813264148
4	22.336629931220845	20.79717807356704	34.35017200381109	22.516019991401027
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.4768843468995	0.32611335183899126	0.1398767882235473	0.057125513037969596
12	99.05378210477659	0.6680689680189515	0.15147888850517782	0.12667003869927715
13	96.5165593480495	1.9323964191620018	0.507263663612614	1.043780569175894
14	53.48362056716862	25.215963906478706	7.3546618123919965	13.94575371396067
15	34.57881993316684	23.207322335424013	19.64717944967533	22.56667828173381
16	31.84598557118851	16.633852400083597	26.954761825802976	24.565400202924916
17	24.458812592625947	16.720435383157657	29.119122449422186	29.701629574794207
18	22.33586164461376	19.81519108795316	32.942029079939836	24.906918187493247
19	27.26033566733618	19.245803185783082	29.766574105959947	23.727287040920793
20	31.284698716407032	18.913990897845988	25.83642828029935	23.96488210544763
21	31.584826475662187	20.60467851533184	23.01841135640417	24.7920836526018
22	30.793578796690063	21.404085592574113	24.29629566236079	23.50603994837503
23	28.97837039797538	23.85453086328865	22.297087483823432	24.870011254912537
24	28.388141505944837	25.95300361649039	21.97064347698899	23.68821140057579
25	28.091543975023487	23.218029722188536	25.157817253013604	23.532609049774372
26	27.52847741835472	21.32288061574962	25.316220446129446	25.832421519766214
27	29.831615888594943	22.098519433906976	23.685206330175934	24.384658347322148
28	32.26672460260861	21.200032559791907	21.313865404550068	25.219377433049416
29	30.926035297809772	20.342984536724636	20.15645621870144	28.574523946764156
30	26.82630236006948	20.901295496547036	22.676173710963383	29.5962284324201
31	24.84509542849046	21.41929572236495	22.570967071527775	31.164641777616815
32	25.963487324875633	25.323485130882485	20.724735454122055	27.988292090119828
33	30.084761463054992	24.58089236197658	20.453588632703664	24.88075754226476
34	28.875896524825723	23.04654620645845	23.640869385344455	24.43668788337137
35	31.950861555628666	22.22711505169742	22.50518617772648	23.316837214947434
36	34.41104169841522	21.21396869533556	21.160626264451444	23.214363341797778
37	32.79896244352486	21.707675504134595	22.691704770602747	22.801657281737796
38	28.892322297982197	24.07651706700072	25.652418775329757	21.37874185968733
39	27.221853260953605	27.31186922089222	25.247881838686848	20.218395679467328
40	28.539056335928358	29.335312465566904	20.783232212876467	21.342398985628275
41	30.50449880434181	27.62721683508514	20.180574583075657	21.687709777497393
42	30.241550281781272	27.394377367404672	19.62575495099936	22.738317399814697
43	29.78383624175198	25.143696339678375	20.010238634682732	25.06222878388691
44	28.397691600225272	24.493122910977657	20.354275432304664	26.754910056492403
45	24.97029697000405	25.632589200749496	22.172556977447677	27.224556851798777
46	25.316852580679576	24.38107949325371	22.311247297746363	27.990820628320346
47	26.4189451308183	21.712226872895542	22.182350200401242	29.68647779588492
48	27.36507549971938	21.530998759946517	21.139678297974807	29.964247442359298
49	28.810951993659984	22.494906697426657	21.5485137495586	27.14562755935476
50	27.665990995875468	25.300407357229247	20.803761998035323	26.229839648859954
51	25.85928237557331	25.185067114697702	22.335472638736757	26.62017787099223
52	26.64397530551792	23.640431753732827	23.586437738004737	26.129155202744514
53	28.01710770045886	24.413230828988056	22.813512235839482	24.7561492347136
54	25.632375247517146	23.56771683017394	23.087226496046096	27.71268142626282
55	24.52754020594555	24.41086761828526	23.97113537492046	27.090456800848735
56	24.46883921910571	23.88767416400935	25.003343019255496	26.64014359762944
57	25.25697485106181	22.796590480189828	26.300103835393717	25.646330833354646
58	25.560739815266942	22.57440004839233	25.495104798669523	26.3697553376712
59	24.98069315206697	22.88907662744742	23.219293991288797	28.910936229196814
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	13.0
5	23.0
6	45.5
7	68.0
8	312.5
9	1138.0
10	1719.0
11	2780.0
12	3841.0
13	5115.0
14	6812.0
15	7235.0
16	7652.0
17	8069.0
18	9645.5
19	12168.5
20	13115.0
21	16399.0
22	19683.0
23	25142.5
24	30602.0
25	37824.0
26	50433.0
27	55820.0
28	64893.0
29	73966.0
30	81339.5
31	93036.0
32	97359.0
33	100583.0
34	103807.0
35	116485.5
36	138878.5
37	148593.0
38	165051.0
39	181509.0
40	196742.5
41	211976.0
42	241301.5
43	303439.0
44	336251.0
45	344954.0
46	353657.0
47	384633.5
48	447909.5
49	480209.0
50	510256.5
51	540304.0
52	591519.5
53	689206.0
54	735677.0
55	839210.5
56	942744.0
57	965865.0
58	937772.0
59	886558.0
60	772285.5
61	658013.0
62	581346.5
63	504680.0
64	433395.0
65	318327.0
66	274544.0
67	234787.5
68	195031.0
69	174774.5
70	137306.0
71	120094.0
72	101511.5
73	82929.0
74	65178.5
75	36251.0
76	25074.0
77	19412.0
78	13750.0
79	10613.0
80	7476.0
81	5298.0
82	2156.0
83	1192.0
84	799.0
85	406.0
86	291.0
87	161.0
88	146.0
89	119.0
90	92.0
91	48.5
92	2.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1.0282621E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.556092133226487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.68640980135893	10.131059953651313
2	7.286821268886308	1.8298799842098163
3	3.0643548853088527	1.154289668065219
4	1.7602323777745454	0.8840655964490209
5	1.0389497835156698	0.6522574601809231
6	0.7016960963343826	0.5286336501059926
7	0.4772578173191061	0.41947451878928904
8	0.32549940294985974	0.32696003941989216
9	0.24476035843117583	0.27659102499210436
>10	1.7107385713672076	4.529199606597056
>50	0.5955194016936051	5.563799231728407
>100	1.8949263509420489	54.681850244028475
>500	0.19209460609726553	15.77677685395387
>1k	0.020739278021365062	3.2451621678289015
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0021200820296692837	0.0	0.0	0.0	0.0
12	0.0029467195183018025	0.0	0.0	0.0	0.0
13	0.006097667122030463	0.0	0.0	0.0	0.0
14	0.03265704337444704	0.0	0.0	0.0	0.0
15	0.06554749027509621	0.0	0.0	0.0	0.0
16	0.08234281901472397	0.0	0.0	0.0	0.0
17	0.09615252764834958	0.0	0.0	0.0	0.0
18	0.11366751726043389	0.0	0.0	0.0	0.0
19	0.13104635481556695	0.0	0.0	0.0	0.0
20	0.1513330113013015	0.0	0.0	0.0	0.0
21	0.16915920561498862	0.0	0.0	0.0	0.0
22	0.187889838592709	0.0	0.0	0.0	0.0
23	0.20768051258526402	0.0	0.0	0.0	0.0
24	0.22938704052206144	0.0	0.0	0.0	0.0
25	0.2508698900795818	0.0	0.0	0.0	0.0
26	0.27545506150620547	0.0	0.0	0.0	0.0
27	0.30108082365381356	0.0	0.0	0.0	0.0
28	0.32768882564085555	0.0	0.0	0.0	0.0
29	0.35498731305957887	0.0	0.0	0.0	0.0
30	0.3871580990877715	0.0	0.0	0.0	0.0
31	0.4164502416261379	0.0	0.0	0.0	0.0
32	0.44464344256196936	0.0	0.0	0.0	0.0
33	0.4671085319589237	0.0	0.0	0.0	0.0
34	0.49007932899598267	0.0	0.0	0.0	0.0
35	0.511124546941874	0.0	0.0	0.0	0.0
36	0.5318391098923124	0.0	0.0	0.0	0.0
37	0.5532733337152075	0.0	0.0	0.0	0.0
38	0.5800952889346014	0.0	0.0	0.0	0.0
39	0.6146390108125156	0.0	0.0	0.0	0.0
40	0.6593455112271472	0.0	0.0	0.0	0.0
41	0.6955522332292515	0.0	0.0	0.0	0.0
42	0.7235412060796561	0.0	0.0	0.0	0.0
43	0.7483306055917066	0.0	0.0	0.0	0.0
44	0.7720988646766228	0.0	0.0	0.0	0.0
45	0.7956823459699623	0.0	0.0	0.0	0.0
46	0.8190226985901746	0.0	0.0	0.0	0.0
47	0.8417892675418067	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCGAT	3720	0.0	49.794357	1
CCGATCT	41045	0.0	49.319893	3
CTACGAT	3010	0.0	49.302322	1
AGATCTT	231485	0.0	49.26114	4
TTGGGGC	160560	0.0	49.124683	9
TACCGAT	3560	0.0	48.98034	1
TTCGATC	31350	0.0	48.917225	2
TAGATCT	86480	0.0	48.81724	3
TTGGGAC	39985	0.0	48.69876	9
TTAGATC	31730	0.0	48.657104	2
GATCTTG	1115660	0.0	48.596233	5
ATGCGAT	3955	0.0	48.57775	1
CTGATCT	98455	0.0	48.53466	3
CGATCTT	252165	0.0	48.51897	4
CGGATCT	51365	0.0	48.516697	3
CTCGATC	18330	0.0	48.48936	2
CGCGATC	10325	0.0	48.482807	2
ATCTTGG	1108750	0.0	48.482037	6
TCTTGGG	1072200	0.0	48.47014	7
CTTGGGG	581115	0.0	48.36547	8
>>END_MODULE