FastQCFastQC Report
Fri 17 Jun 2016
SRR1527987_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527987_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323458
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGGCGAATGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCC28630.21632722761130313No Hit
GGTAGCGAATGGGAGGATGGCTCCTCTGCTGCTCAGCAGACAGTTTGGAGAG19200.1450744942416004No Hit
ACTGGCGAATGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCAT18560.1402386777668804No Hit
TTGTGCGAATGGGGCCCCCTTCCACTTGGTCACGTTGAGCCGCTCCCGGGCC18150.1371407328377629No Hit
TGGCGCGAATGGGGTACCCCATCTCTACTAAAAATACAAAAATTAGCTGGGC18120.1369140539405104No Hit
TATTGCGAATGGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATC17980.1358562190866654No Hit
TAATGCGAATGGGACACTGTCCTGGGGAGCTCGGCCCGGCAGGAGCTGCACG17940.13555398055699538No Hit
CTTTGCGAATGGGGGAAGCCAAGGCGAGCAGGGGATCCAAGGTCCATCTGGT17080.12905585216909035No Hit
AGTAGCGAATGGGATCGTGGACATGCTCTACCTCAAAGACTTGGCTATCGTT16360.12361555863503035No Hit
GGCTGCGAATGGGAGCCAGGGTATTGTGGCTCAGGCCTGTAGTCCTAGGACT16000.12089541186800035No Hit
TTAAGCGAATGGGCAAGGCAAAAAAGCACACCCACGACCTTACCAACACCAA15840.11968645774932034No Hit
CCTTGCGAATGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGA14320.1082013936218603No Hit
ACATGCGAATGGGCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGC14070.1063124028114228No Hit
CAGGGCGAATGGTCTGATGCTGTGGCTTAATTAAGAGCACCCTCTGGGCCGG13650.10313889824988778No Hit
TGCTGCGAATGGGGTTGCAAACTTGACAGGCCAGATCCCCTTGTAAGTTTCT13500.10200550376362529No Hit
GGCCGCGAATGGGCGTGGCTGCACACTGCCCTCTGCAGGCCACATGGAGTAG13420.1014010267042853No Hit
GGCAGCGAATGGGGGCCTGGAGCTGCGGCACGTGTGCACAGGTCCCAACCCC13340.10079654964494529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTAGG351.01972546E-746.00000425
TCGCCCG551.8189894E-1246.00000426
AACGTTC351.01972546E-746.00000446
AGCGTAT551.8189894E-1246.00000418
CTGTCGG551.8189894E-1246.00000419
ACCGGTT551.8189894E-1246.00000445
CGAACTA351.01972546E-746.00000426
GTTTCGC351.01972546E-746.00000418
CGAAACC551.8189894E-1246.00000444
CGTGCCG700.046.00000430
TAGCGTA551.8189894E-1246.00000417
GACCGTC351.01972546E-746.00000437
CCTACGC750.046.00000430
AGGAATA551.8189894E-1246.00000420
CACGAAG351.01972546E-746.00000419
GAATAAT351.01972546E-746.00000423
ATCCGGT351.01972546E-746.00000423
CTAACGG750.046.00000426
CCCGTAC551.8189894E-1246.00000420
CTTATCG750.046.00000417
Produced by FastQC (version 0.11.5)