##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527987_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1323458
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.770011590847613	31.0	31.0	34.0	30.0	34.0
2	32.320484669706175	34.0	31.0	34.0	30.0	34.0
3	32.46842060722743	34.0	31.0	34.0	30.0	34.0
4	35.882059725355845	37.0	35.0	37.0	35.0	37.0
5	35.923796599514304	37.0	35.0	37.0	35.0	37.0
6	35.87847593199029	37.0	35.0	37.0	35.0	37.0
7	35.97742353742998	37.0	35.0	37.0	35.0	37.0
8	35.631679282606626	37.0	35.0	37.0	32.0	37.0
9	37.37505761421972	39.0	37.0	39.0	34.0	39.0
10	37.75116021815577	39.0	38.0	39.0	35.0	39.0
11	38.08235924373875	39.0	38.0	39.0	37.0	39.0
12	38.13363476589359	39.0	39.0	39.0	37.0	39.0
13	38.14805078816253	39.0	39.0	39.0	37.0	39.0
14	38.70314207175445	40.0	39.0	40.0	36.0	40.0
15	38.108549723527304	40.0	38.0	40.0	34.0	40.0
16	37.62912990060886	40.0	37.0	40.0	33.0	40.0
17	37.58707340920528	40.0	37.0	40.0	33.0	40.0
18	37.60406828172862	40.0	37.0	40.0	33.0	40.0
19	37.72109277362787	40.0	37.0	40.0	33.0	40.0
20	37.7696609941532	40.0	37.0	40.0	33.0	40.0
21	37.590791698716544	40.0	37.0	40.0	33.0	40.0
22	37.65417111838834	40.0	37.0	40.0	33.0	40.0
23	37.59787692544833	40.0	37.0	40.0	33.0	40.0
24	37.58130367567388	40.0	37.0	40.0	33.0	40.0
25	37.70686489484366	40.0	37.0	40.0	33.0	40.0
26	37.67634333692494	40.0	37.0	40.0	33.0	40.0
27	37.589539675607384	40.0	37.0	40.0	33.0	40.0
28	37.548981531714645	40.0	37.0	40.0	33.0	40.0
29	37.51487013565976	40.0	37.0	40.0	33.0	40.0
30	37.47298138664015	40.0	36.0	40.0	33.0	40.0
31	37.3898000541007	40.0	36.0	40.0	33.0	40.0
32	37.28954980059813	40.0	36.0	40.0	33.0	40.0
33	37.20982532124178	40.0	36.0	40.0	33.0	40.0
34	37.12355057735115	40.0	35.0	40.0	33.0	40.0
35	37.030638675348975	40.0	35.0	40.0	32.0	40.0
36	36.94031997993136	40.0	35.0	40.0	32.0	40.0
37	36.7667700826169	39.0	35.0	40.0	32.0	40.0
38	36.51914227727665	39.0	35.0	40.0	31.0	40.0
39	36.33802508277558	39.0	35.0	40.0	31.0	40.0
40	36.04567579779638	39.0	35.0	40.0	30.0	40.0
41	35.82873502596985	38.0	35.0	40.0	30.0	40.0
42	35.59251219154669	38.0	35.0	40.0	29.0	40.0
43	35.32747771368642	38.0	35.0	40.0	28.0	40.0
44	35.09168254678275	38.0	35.0	40.0	27.0	40.0
45	34.8418544449465	37.0	34.0	40.0	26.0	40.0
46	34.629092876388974	37.0	34.0	40.0	26.0	40.0
47	34.44330836339348	37.0	34.0	40.0	25.0	40.0
48	34.251609797968655	36.0	34.0	40.0	24.0	40.0
49	34.017353025181	36.0	34.0	39.0	24.0	40.0
50	33.80970986612344	36.0	34.0	39.0	23.0	40.0
51	33.54245469066642	35.0	33.0	39.0	23.0	40.0
52	32.80909934429351	35.0	33.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	7.0
15	11.0
16	42.0
17	116.0
18	290.0
19	616.0
20	1019.0
21	1744.0
22	2672.0
23	3956.0
24	5885.0
25	8191.0
26	11130.0
27	14422.0
28	18947.0
29	24281.0
30	30260.0
31	34789.0
32	38056.0
33	45592.0
34	62618.0
35	104996.0
36	118594.0
37	217820.0
38	397445.0
39	179956.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.832951253458745	21.55391406451886	34.67824441727656	14.93489026474584
2	23.419708067804194	20.80360691461308	34.591501959261265	21.185183058321456
3	25.610786288646864	20.531743357174918	35.48189666766909	18.37557368650913
4	26.602808702656223	18.251429210447174	34.11955649518156	21.026205591715037
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	100.0	0.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.62552646173887	0.2434531356491857	0.10230774229329528	0.028712660318650083
12	99.5950003702422	0.29407808936891083	0.06702139395432269	0.043900146434567626
13	98.1524914277597	1.1182070001465858	0.3864875198155136	0.3428140522781985
14	54.440715156808906	29.708158475750647	5.895086961580949	9.9560394058595
15	29.837289887552153	27.36580987080814	20.55977598080181	22.237124260837895
16	29.19699756244626	20.77980563040157	28.812625712338434	21.210571094813737
17	23.36477621503667	20.76106684156203	29.877563171630683	25.99659377177062
18	20.47537587139146	24.12581283274573	31.964898017164128	23.433913278698682
19	21.83748936498174	24.87309759735481	31.102913730545286	22.186499307118172
20	24.88057800096414	24.194723217510493	28.913195583086125	22.01150319843924
21	25.974228120575038	25.177300677467663	22.744733871418664	26.103737330538635
22	27.502572805483815	23.80030193629114	24.223133639299473	24.473991618925574
23	26.324824814992244	25.796360745864245	23.068733575224904	24.810080863918614
24	27.182124404401197	27.363543081835616	22.359908663516332	23.094423850246855
25	29.025854995020623	25.540515830498588	21.9957867948964	23.437842379584392
26	26.99307420409261	25.59189638054249	21.7073756779588	25.7076537374061
27	27.47129111766297	28.295268909175807	22.65655578038744	21.57688419277378
28	29.785984897140672	25.919598506337188	19.813397931781743	24.4810186647404
29	29.01580556390909	25.716569774031363	19.960210297568945	25.307414364490597
30	28.96450057349761	28.281063698281322	18.916882893148102	23.837552835072966
31	25.95488485467616	27.60299155696667	20.89979432668056	25.542329261676606
32	24.979787798328317	30.850166760108742	21.253942323821384	22.916103117741553
33	27.599742492772723	28.848743216634	20.842897923470183	22.708616367123096
34	26.595177179782052	29.09264971007769	19.951445380208515	24.36072772993174
35	24.758020277182954	29.981230987307494	19.617622924187998	25.643125811321553
36	28.463464650937166	30.04900797758599	20.619090292249545	20.8684370792273
37	28.21396674469458	29.92962375836634	19.385654852666274	22.470754644272805
38	27.34828003608728	30.20768320566274	20.215526295507676	22.2285104627423
39	27.957063994475078	28.606347915838658	21.833711383360864	21.6028767063254
40	27.079741102475484	28.353903183931788	21.35496555236358	23.211390161229144
41	27.841004399081797	31.31999655448076	19.498012026071095	21.34098702036634
42	29.472941340034968	27.707188290070405	19.745545381870826	23.074324988023797
43	27.68013794166494	27.538916988676633	21.14702544395062	23.633919625707804
44	26.94630279162618	28.305545019184592	21.16546199426049	23.58269019492874
45	24.54781337979747	29.197450920240765	22.667587486720393	23.58714821324137
46	26.53646734539366	29.020490261118976	20.91883535404977	23.524207039437595
47	28.082719663185387	27.81198950023348	20.600049264880337	23.505241571700804
48	29.410000166231193	26.833643379691686	20.727442805136242	23.02891364894088
49	27.272493724772527	26.412398428964124	21.688788008384098	24.62631983787925
50	26.503296666762377	28.52542354951952	20.736736639923596	24.234543143794514
51	25.72389905837586	28.982181527483302	21.869980006921264	23.42393940721957
52	27.112005065517756	27.313522605175226	21.17755153544729	24.39692079385972
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	8.0
10	15.0
11	48.0
12	81.0
13	240.5
14	622.5
15	845.0
16	968.0
17	1091.0
18	1543.5
19	1996.0
20	2533.0
21	3070.0
22	3876.0
23	4682.0
24	6803.0
25	8924.0
26	10589.0
27	12254.0
28	15167.0
29	18080.0
30	19195.5
31	20311.0
32	22078.0
33	23845.0
34	25001.0
35	26157.0
36	26743.0
37	27329.0
38	28012.0
39	29115.0
40	29535.0
41	32361.0
42	35187.0
43	41269.5
44	47352.0
45	54618.5
46	61885.0
47	62232.5
48	62580.0
49	67338.5
50	72097.0
51	78491.0
52	84885.0
53	86110.0
54	87335.0
55	87577.0
56	87819.0
57	96366.5
58	104914.0
59	110549.5
60	116185.0
61	106424.0
62	96663.0
63	89653.5
64	71842.5
65	61041.0
66	53348.5
67	45656.0
68	36874.0
69	28092.0
70	23279.0
71	18466.0
72	14542.0
73	10618.0
74	8298.5
75	5979.0
76	5481.0
77	4983.0
78	3290.5
79	1598.0
80	851.5
81	105.0
82	73.0
83	41.0
84	24.5
85	8.0
86	7.0
87	6.0
88	5.0
89	4.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1323458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.031464819155634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.93204876017047	11.49626659739676
2	6.199204632352353	1.7396784341119735
3	2.7409182077227703	1.153772922115355
4	1.6756303573925733	0.9404619363865229
5	1.0907874192686482	0.76526726493228
6	0.7733550542863823	0.6510782542161341
7	0.5775965189831761	0.5673167665045437
8	0.4178317850709667	0.4690233594038613
9	0.32033815101603086	0.4045332146593321
>10	2.106724331223769	5.7127074443294745
>50	0.4347391628473864	4.506185979451866
>100	1.5329705143795611	51.20889226117982
>500	0.17834062914811727	16.534572667753935
>1k	0.01951447613778791	3.8502428975581546
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTGGCGAATGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCC	2863	0.21632722761130313	No Hit
GGTAGCGAATGGGAGGATGGCTCCTCTGCTGCTCAGCAGACAGTTTGGAGAG	1920	0.1450744942416004	No Hit
ACTGGCGAATGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCAT	1856	0.1402386777668804	No Hit
TTGTGCGAATGGGGCCCCCTTCCACTTGGTCACGTTGAGCCGCTCCCGGGCC	1815	0.1371407328377629	No Hit
TGGCGCGAATGGGGTACCCCATCTCTACTAAAAATACAAAAATTAGCTGGGC	1812	0.1369140539405104	No Hit
TATTGCGAATGGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATC	1798	0.1358562190866654	No Hit
TAATGCGAATGGGACACTGTCCTGGGGAGCTCGGCCCGGCAGGAGCTGCACG	1794	0.13555398055699538	No Hit
CTTTGCGAATGGGGGAAGCCAAGGCGAGCAGGGGATCCAAGGTCCATCTGGT	1708	0.12905585216909035	No Hit
AGTAGCGAATGGGATCGTGGACATGCTCTACCTCAAAGACTTGGCTATCGTT	1636	0.12361555863503035	No Hit
GGCTGCGAATGGGAGCCAGGGTATTGTGGCTCAGGCCTGTAGTCCTAGGACT	1600	0.12089541186800035	No Hit
TTAAGCGAATGGGCAAGGCAAAAAAGCACACCCACGACCTTACCAACACCAA	1584	0.11968645774932034	No Hit
CCTTGCGAATGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGA	1432	0.1082013936218603	No Hit
ACATGCGAATGGGCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGC	1407	0.1063124028114228	No Hit
CAGGGCGAATGGTCTGATGCTGTGGCTTAATTAAGAGCACCCTCTGGGCCGG	1365	0.10313889824988778	No Hit
TGCTGCGAATGGGGTTGCAAACTTGACAGGCCAGATCCCCTTGTAAGTTTCT	1350	0.10200550376362529	No Hit
GGCCGCGAATGGGCGTGGCTGCACACTGCCCTCTGCAGGCCACATGGAGTAG	1342	0.1014010267042853	No Hit
GGCAGCGAATGGGGGCCTGGAGCTGCGGCACGTGTGCACAGGTCCCAACCCC	1334	0.10079654964494529	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.014809687953830043	0.0	0.0	0.0	0.0
12	0.019494385163715055	0.0	0.0	0.0	0.0
13	0.040802201505450116	0.0	0.0	0.0	0.0
14	0.09014264147407776	0.0	0.0	0.0	0.0
15	0.1697069344097055	0.0	0.0	0.0	0.0
16	0.22297647526404313	0.0	0.0	0.0	0.0
17	0.2560715942629082	0.0	0.0	0.0	0.0
18	0.2862954472299083	0.0	0.0	0.0	0.0
19	0.3174260157859184	0.0	0.0	0.0	0.0
20	0.357548180599611	0.0	0.0	0.0	0.0
21	0.39502575827869113	0.0	0.0	0.0	0.0
22	0.4406637762588613	0.0	0.0	0.0	0.0
23	0.4819948951912339	0.0	0.0	0.0	0.0
24	0.5280107113334915	0.0	0.0	0.0	0.0
25	0.5826403255713442	0.0	0.0	0.0	0.0
26	0.6558576093839019	0.0	0.0	0.0	0.0
27	0.7231812418678946	0.0	0.0	0.0	0.0
28	0.7945095348700147	0.0	0.0	0.0	0.0
29	0.8770962131023425	0.0	0.0	0.0	0.0
30	0.9811418269412403	0.0	0.0	0.0	0.0
31	1.0704533124587257	0.0	0.0	0.0	0.0
32	1.1374747064130482	0.0	0.0	0.0	0.0
33	1.200566999481661	0.0	0.0	0.0	0.0
34	1.2558010907788535	0.0	0.0	0.0	0.0
35	1.3072572004551712	0.0	0.0	0.0	0.0
36	1.3544064110836913	0.0	0.0	0.0	0.0
37	1.394377456632549	0.0	0.0	0.0	0.0
38	1.438655401229204	0.0	0.0	0.0	0.0
39	1.4903381898027743	0.0	0.0	0.0	0.0
40	1.5402831068307419	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTAGG	35	1.01972546E-7	46.000004	25
TCGCCCG	55	1.8189894E-12	46.000004	26
AACGTTC	35	1.01972546E-7	46.000004	46
AGCGTAT	55	1.8189894E-12	46.000004	18
CTGTCGG	55	1.8189894E-12	46.000004	19
ACCGGTT	55	1.8189894E-12	46.000004	45
CGAACTA	35	1.01972546E-7	46.000004	26
GTTTCGC	35	1.01972546E-7	46.000004	18
CGAAACC	55	1.8189894E-12	46.000004	44
CGTGCCG	70	0.0	46.000004	30
TAGCGTA	55	1.8189894E-12	46.000004	17
GACCGTC	35	1.01972546E-7	46.000004	37
CCTACGC	75	0.0	46.000004	30
AGGAATA	55	1.8189894E-12	46.000004	20
CACGAAG	35	1.01972546E-7	46.000004	19
GAATAAT	35	1.01972546E-7	46.000004	23
ATCCGGT	35	1.01972546E-7	46.000004	23
CTAACGG	75	0.0	46.000004	26
CCCGTAC	55	1.8189894E-12	46.000004	20
CTTATCG	75	0.0	46.000004	17
>>END_MODULE