FastQCFastQC Report
Fri 17 Jun 2016
SRR1527986_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527986_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315045
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGGCGAATGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCC26150.1988525107505827No Hit
TGGCGCGAATGGGGTACCCCATCTCTACTAAAAATACAAAAATTAGCTGGGC18640.14174419886771936No Hit
GGTAGCGAATGGGAGGATGGCTCCTCTGCTGCTCAGCAGACAGTTTGGAGAG18360.13961499416369783No Hit
TATTGCGAATGGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATC17510.1331513370264896No Hit
ACTGGCGAATGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCAT17340.13185860559904794No Hit
TTGTGCGAATGGGGCCCCCTTCCACTTGGTCACGTTGAGCCGCTCCCGGGCC17100.13003357299560092No Hit
TAATGCGAATGGGACACTGTCCTGGGGAGCTCGGCCCGGCAGGAGCTGCACG16950.1288929276184465No Hit
CTTTGCGAATGGGGGAAGCCAAGGCGAGCAGGGGATCCAAGGTCCATCTGGT16510.12554703451212695No Hit
TTAAGCGAATGGGCAAGGCAAAAAAGCACACCCACGACCTTACCAACACCAA15860.12060423787779125No Hit
AGTAGCGAATGGGATCGTGGACATGCTCTACCTCAAAGACTTGGCTATCGTT15280.11619374241946094No Hit
GGCTGCGAATGGGAGCCAGGGTATTGTGGCTCAGGCCTGTAGTCCTAGGACT14710.11185928998627423No Hit
GGCAGCGAATGGGGGCCTGGAGCTGCGGCACGTGTGCACAGGTCCCAACCCC13910.10577584797478413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA351.01972546E-746.00000421
TGATAGG351.01972546E-746.00000431
GACGTAC351.01972546E-746.00000428
ATTACGA351.01972546E-746.00000415
TGAACGT351.01972546E-746.00000416
CGACATT351.01972546E-746.00000430
CGACACG351.01972546E-746.00000445
ACTAGGG351.01972546E-746.00000417
TCTACAC351.01972546E-746.00000427
ATATAAC351.01972546E-746.00000426
CGGGCAT351.01972546E-746.00000445
CTTTCGT351.01972546E-746.00000420
CGCCGTT700.046.00000418
ATACGCC700.046.00000436
TATCGCA351.01972546E-746.00000443
AATACCG351.01972546E-746.00000430
TCGAACG351.01972546E-746.00000418
GTACGTC351.01972546E-746.00000413
TGCGTCA351.01972546E-746.00000425
TACAACG351.01972546E-746.00000426
Produced by FastQC (version 0.11.5)