##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527986_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1315045
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71374819873084	31.0	31.0	34.0	30.0	34.0
2	32.1563475014163	34.0	31.0	34.0	30.0	34.0
3	32.39497127474725	34.0	31.0	34.0	30.0	34.0
4	35.84900364626306	37.0	35.0	37.0	35.0	37.0
5	35.90639483819945	37.0	35.0	37.0	35.0	37.0
6	35.861902064187916	37.0	35.0	37.0	35.0	37.0
7	35.98061283073963	37.0	35.0	37.0	35.0	37.0
8	35.574879186643805	37.0	35.0	37.0	32.0	37.0
9	37.35301681691501	39.0	37.0	39.0	34.0	39.0
10	37.738381576295865	39.0	38.0	39.0	35.0	39.0
11	38.063711127756086	39.0	38.0	39.0	35.0	39.0
12	38.10343980624237	39.0	39.0	39.0	37.0	39.0
13	38.14752042705763	39.0	39.0	39.0	37.0	39.0
14	38.72718728256447	40.0	39.0	40.0	36.0	40.0
15	38.13886216821478	40.0	38.0	40.0	34.0	40.0
16	37.66921892406724	40.0	37.0	40.0	33.0	40.0
17	37.64482204031041	40.0	37.0	40.0	33.0	40.0
18	37.67841024451635	40.0	37.0	40.0	33.0	40.0
19	37.788725100661956	40.0	37.0	40.0	33.0	40.0
20	37.81575839610051	40.0	37.0	40.0	33.0	40.0
21	37.83339277363132	40.0	37.0	40.0	33.0	40.0
22	37.79307932428168	40.0	37.0	40.0	33.0	40.0
23	37.70202996855621	40.0	37.0	40.0	33.0	40.0
24	37.68481382766369	40.0	37.0	40.0	33.0	40.0
25	37.65327954556688	40.0	37.0	40.0	33.0	40.0
26	37.60458767570692	40.0	37.0	40.0	33.0	40.0
27	37.54603910892783	40.0	37.0	40.0	33.0	40.0
28	37.353248748141695	40.0	36.0	40.0	33.0	40.0
29	37.42589265006141	40.0	36.0	40.0	33.0	40.0
30	37.457506777334615	40.0	36.0	40.0	33.0	40.0
31	37.415229136645515	40.0	36.0	40.0	33.0	40.0
32	37.313784699382914	40.0	36.0	40.0	33.0	40.0
33	37.22736940560969	40.0	36.0	40.0	33.0	40.0
34	37.16367272602839	40.0	35.0	40.0	33.0	40.0
35	37.00575569657312	40.0	35.0	40.0	32.0	40.0
36	36.52853704626077	39.0	35.0	40.0	31.0	40.0
37	36.57990334931504	39.0	35.0	40.0	31.0	40.0
38	36.46079867989308	39.0	35.0	40.0	31.0	40.0
39	36.31741347254277	39.0	35.0	40.0	31.0	40.0
40	36.04468212114414	39.0	35.0	40.0	30.0	40.0
41	35.84314453117574	39.0	35.0	40.0	30.0	40.0
42	35.46710340710774	38.0	35.0	40.0	28.0	40.0
43	35.21097300852823	38.0	35.0	40.0	27.0	40.0
44	34.98506210814079	37.0	35.0	40.0	26.0	40.0
45	34.49478991213228	37.0	34.0	40.0	24.0	40.0
46	34.43059134858503	37.0	34.0	40.0	24.0	40.0
47	34.32532574930896	37.0	34.0	40.0	24.0	40.0
48	34.13208521381397	36.0	34.0	40.0	23.0	40.0
49	33.90594922607211	36.0	34.0	39.0	23.0	40.0
50	33.641973468588525	36.0	33.0	39.0	22.0	40.0
51	33.46858928781905	35.0	33.0	39.0	22.0	40.0
52	32.8817196369706	35.0	33.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	4.0
14	4.0
15	11.0
16	43.0
17	113.0
18	274.0
19	578.0
20	1039.0
21	1761.0
22	2720.0
23	3868.0
24	5891.0
25	8151.0
26	10941.0
27	15099.0
28	19238.0
29	24741.0
30	30394.0
31	35285.0
32	38679.0
33	46072.0
34	63411.0
35	104660.0
36	120260.0
37	219515.0
38	384688.0
39	177601.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.780688113334524	21.55348296065914	34.635392705192594	15.030436220813737
2	23.544669574044992	20.94285746875582	34.347113596873115	21.16535936032607
3	25.736229558684304	20.484470113190042	35.42951001676749	18.349790311358166
4	26.64996254881012	18.16074735085111	34.24110961982289	20.948180480515877
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	100.0	0.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.60716173210803	0.2530711876779882	0.1088936120056728	0.030873468208312264
12	99.58647802926896	0.2881270222691999	0.0845598439597124	0.0408351045021273
13	98.17200171857236	1.0965404225710909	0.3815839001707166	0.34987395868582444
14	54.48771715036368	29.680885444984774	5.888695824097274	9.942701580554278
15	29.87433890095016	27.422939899395075	20.499146417042763	22.203574782612
16	29.186833910626632	20.818527122645992	28.770574390990422	21.22406457573695
17	23.3972221482915	20.781418126375904	29.831070419643435	25.990289305689156
18	20.554733868422755	24.107007744982113	31.94286127090708	23.39539711568806
19	21.761536677452103	24.969639822211406	31.06228303974389	22.206540460592603
20	24.853978380967952	24.279245196932425	28.889125467189338	21.97765095491029
21	25.579961142014152	25.29806964780673	22.862183423380948	26.25978578679817
22	27.504990323525053	23.868232646031124	24.183507028276598	24.443270002167225
23	26.402138329867036	25.87014132596223	22.97183746563806	24.755882878532674
24	27.13359618872358	27.360584618777306	22.4111722412541	23.094646951245014
25	29.041591732602306	25.523765346433013	21.997346098422486	23.437296822542194
26	26.923793482352316	25.69273294830215	21.673630940386072	25.709842628959468
27	27.439289149800956	28.33591245926945	22.653217190286266	21.571581200643326
28	29.889623549004025	25.756228874297076	19.84738164853674	24.506765928162157
29	28.946994209323634	25.823146736423467	19.9057066488219	25.324152405430993
30	28.94395248831789	28.323593489196185	18.89235729575794	23.84009672672798
31	25.880483177381763	27.665441106578104	20.921793550791037	25.5322821652491
32	24.964316810451354	30.949815405556464	21.1877160097183	22.898151774273884
33	27.741256002646296	28.864943785193663	20.7922162359463	22.60158397621374
34	26.620990156230395	29.10470744347152	19.964487907257926	24.309814493040165
35	24.82074757898019	30.007490237976647	19.59066039565186	25.581101787391308
36	28.596435863411518	29.81145131915638	20.711230414168334	20.88088240326377
37	28.200860046614373	29.993954579501082	19.355459318882623	22.44972605500192
38	27.363778425833335	30.245885121801912	20.208357888893534	22.181978563471212
39	27.914482013923482	28.70768680919664	21.746023900322804	21.631807276557076
40	26.97405792197225	28.437125725735623	21.402765684824473	23.186050667467654
41	27.86307692892639	31.311552076164695	19.564577638027597	21.260793356881326
42	29.62263648772476	27.737301765338827	19.718260591842864	22.92180115509355
43	27.59076685588706	27.57129984145029	21.168401081331815	23.66953222133083
44	27.125079369907496	28.20793204795273	21.102547821557437	23.564440760582336
45	24.632160876623992	29.171169047447044	22.73070503290762	23.465965043021342
46	26.601903356919344	28.95437038276257	20.940804307076945	23.50292195324114
47	28.039040489108736	27.779809816394113	20.629712291214368	23.551437403282776
48	29.53070047032611	26.812314407491762	20.65214498363174	23.004840138550392
49	27.440962096354117	26.375675357117057	21.783437068693466	24.39992547783536
50	26.71938983076625	28.44693527597915	20.687276861248094	24.14639803200651
51	25.67653578394656	29.066685930899705	21.83263690596139	23.42414137919235
52	27.095726762202055	27.384005870521538	21.123459653471937	24.39680771380447
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	2.0
9	7.0
10	12.0
11	39.0
12	66.0
13	209.0
14	575.5
15	799.0
16	970.5
17	1142.0
18	1570.0
19	1998.0
20	2477.5
21	2957.0
22	3854.0
23	4751.0
24	6808.0
25	8865.0
26	10416.5
27	11968.0
28	14895.0
29	17822.0
30	19050.5
31	20279.0
32	22182.5
33	24086.0
34	25067.0
35	26048.0
36	26852.0
37	27656.0
38	28284.5
39	29141.0
40	29369.0
41	31975.5
42	34582.0
43	40801.0
44	47020.0
45	53934.5
46	60849.0
47	61552.5
48	62256.0
49	66900.0
50	71544.0
51	77702.5
52	83861.0
53	85305.0
54	86749.0
55	87558.5
56	88368.0
57	96000.5
58	103633.0
59	109331.5
60	115030.0
61	105585.5
62	96141.0
63	89087.5
64	71606.5
65	61179.0
66	53176.0
67	45173.0
68	36500.5
69	27828.0
70	23231.0
71	18634.0
72	14522.0
73	10410.0
74	8188.0
75	5966.0
76	5421.0
77	4876.0
78	3261.5
79	1647.0
80	877.5
81	108.0
82	77.0
83	46.0
84	30.0
85	14.0
86	11.0
87	8.0
88	6.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1315045.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.09746550200099
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.83190453524185	12.354543556838522
2	6.571393936673083	1.9842278651796066
3	2.865312418158769	1.2977686615682114
4	1.702222998704667	1.0279701199862552
5	1.0911233082151306	0.8236598252103565
6	0.7297788288053756	0.6610686415187906
7	0.5271109993629043	0.55706280900247
8	0.41592975699099805	0.5023588125941798
9	0.2864468142423043	0.38921588065631474
>10	1.923256961919743	5.609449319377795
>50	0.4345718041653193	4.837845303214807
>100	1.4516878906167592	51.29403124357851
>500	0.15212609154174192	15.103157614346108
>1k	0.0171336553613566	3.5576403469280677
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTGGCGAATGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCC	2615	0.1988525107505827	No Hit
TGGCGCGAATGGGGTACCCCATCTCTACTAAAAATACAAAAATTAGCTGGGC	1864	0.14174419886771936	No Hit
GGTAGCGAATGGGAGGATGGCTCCTCTGCTGCTCAGCAGACAGTTTGGAGAG	1836	0.13961499416369783	No Hit
TATTGCGAATGGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATC	1751	0.1331513370264896	No Hit
ACTGGCGAATGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCAT	1734	0.13185860559904794	No Hit
TTGTGCGAATGGGGCCCCCTTCCACTTGGTCACGTTGAGCCGCTCCCGGGCC	1710	0.13003357299560092	No Hit
TAATGCGAATGGGACACTGTCCTGGGGAGCTCGGCCCGGCAGGAGCTGCACG	1695	0.1288929276184465	No Hit
CTTTGCGAATGGGGGAAGCCAAGGCGAGCAGGGGATCCAAGGTCCATCTGGT	1651	0.12554703451212695	No Hit
TTAAGCGAATGGGCAAGGCAAAAAAGCACACCCACGACCTTACCAACACCAA	1586	0.12060423787779125	No Hit
AGTAGCGAATGGGATCGTGGACATGCTCTACCTCAAAGACTTGGCTATCGTT	1528	0.11619374241946094	No Hit
GGCTGCGAATGGGAGCCAGGGTATTGTGGCTCAGGCCTGTAGTCCTAGGACT	1471	0.11185928998627423	No Hit
GGCAGCGAATGGGGGCCTGGAGCTGCGGCACGTGTGCACAGGTCCCAACCCC	1391	0.10577584797478413	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.013611701500709101	0.0	0.0	0.0	0.0
12	0.018402412084757555	0.0	0.0	0.0	0.0
13	0.03862985677296214	0.0	0.0	0.0	0.0
14	0.09300061975065492	0.0	0.0	0.0	0.0
15	0.1714009786737336	0.0	0.0	0.0	0.0
16	0.22250189157025044	0.0	0.0	0.0	0.0
17	0.25748168313631853	0.0	0.0	0.0	0.0
18	0.28934371067149794	0.0	0.0	0.0	0.0
19	0.32417141618727874	0.0	0.0	0.0	0.0
20	0.36310544506081543	0.0	0.0	0.0	0.0
21	0.4031040762863628	0.0	0.0	0.0	0.0
22	0.44576421339193717	0.0	0.0	0.0	0.0
23	0.4868274469694953	0.0	0.0	0.0	0.0
24	0.5313886597036603	0.0	0.0	0.0	0.0
25	0.585987551756784	0.0	0.0	0.0	0.0
26	0.6532856290088932	0.0	0.0	0.0	0.0
27	0.7216483086130132	0.0	0.0	0.0	0.0
28	0.7883380416639735	0.0	0.0	0.0	0.0
29	0.8717572402465315	0.0	0.0	0.0	0.0
30	0.9830842290568004	0.0	0.0	0.0	0.0
31	1.070837880072545	0.0	0.0	0.0	0.0
32	1.1390484736263777	0.0	0.0	0.0	0.0
33	1.2014037542441514	0.0	0.0	0.0	0.0
34	1.25296092529153	0.0	0.0	0.0	0.0
35	1.3017045044085944	0.0	0.0	0.0	0.0
36	1.3471021904193392	0.0	0.0	7.604302514362627E-5	0.0
37	1.3922717473546533	0.0	0.0	7.604302514362627E-5	0.0
38	1.435464185636233	0.0	0.0	7.604302514362627E-5	0.0
39	1.4853484101304517	0.0	0.0	7.604302514362627E-5	0.0
40	1.5368295381526869	0.0	0.0	7.604302514362627E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	35	1.01972546E-7	46.000004	21
TGATAGG	35	1.01972546E-7	46.000004	31
GACGTAC	35	1.01972546E-7	46.000004	28
ATTACGA	35	1.01972546E-7	46.000004	15
TGAACGT	35	1.01972546E-7	46.000004	16
CGACATT	35	1.01972546E-7	46.000004	30
CGACACG	35	1.01972546E-7	46.000004	45
ACTAGGG	35	1.01972546E-7	46.000004	17
TCTACAC	35	1.01972546E-7	46.000004	27
ATATAAC	35	1.01972546E-7	46.000004	26
CGGGCAT	35	1.01972546E-7	46.000004	45
CTTTCGT	35	1.01972546E-7	46.000004	20
CGCCGTT	70	0.0	46.000004	18
ATACGCC	70	0.0	46.000004	36
TATCGCA	35	1.01972546E-7	46.000004	43
AATACCG	35	1.01972546E-7	46.000004	30
TCGAACG	35	1.01972546E-7	46.000004	18
GTACGTC	35	1.01972546E-7	46.000004	13
TGCGTCA	35	1.01972546E-7	46.000004	25
TACAACG	35	1.01972546E-7	46.000004	26
>>END_MODULE