FastQCFastQC Report
Fri 17 Jun 2016
SRR1527985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1330338
Sequences flagged as poor quality0
Sequence length59
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGGCGAATGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTGTCT27170.2042338112569888No Hit
ACTGGCGAATGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAGGCC18390.13823554615443595No Hit
GGTAGCGAATGGGAGGATGGCTCCTCTGCTGCTCAGCAGACAGTTTGGAGAGCTTTCTC18310.13763419521956075No Hit
TATTGCGAATGGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTGTC18190.13673216881724795No Hit
TGGCGCGAATGGGGTACCCCATCTCTACTAAAAATACAAAAATTAGCTGGGCATAATGG17340.13034281513419899No Hit
TTGTGCGAATGGGGCCCCCTTCCACTTGGTCACGTTGAGCCGCTCCCGGGCCTGGCATC16650.1251561633209004No Hit
TAATGCGAATGGGACACTGTCCTGGGGAGCTCGGCCCGGCAGGAGCTGCACGCACCTCG16560.12447964351916582No Hit
CTTTGCGAATGGGGGAAGCCAAGGCGAGCAGGGGATCCAAGGTCCATCTGGTCCACCTG16050.12064603130933643No Hit
TTAAGCGAATGGGCAAGGCAAAAAAGCACACCCACGACCTTACCAACACCAAAGAAACA15680.11786478323553863No Hit
GGCTGCGAATGGGAGCCAGGGTATTGTGGCTCAGGCCTGTAGTCCTAGGACTTCACAGT14920.11215194935422428No Hit
AGTAGCGAATGGGATCGTGGACATGCTCTACCTCAAAGACTTGGCTATCGTTGAGCCCG14280.10734114187522269No Hit
ACATGCGAATGGGCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCTACCCCA14160.10643911547290989No Hit
CCTTGCGAATGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCC14060.1056874268043159No Hit
CCTAGCGAATGGGGAGAAACTGCCAGCCGAGATCACAAGCATTGGGGCTGAAGCAGGAG13800.10373303626597151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA550.053.00000439
ATAGACG1100.053.00000414
ATCACAC750.053.00000448
ACGATTG750.053.00000425
ATATCGA550.053.00000415
TACGCCC550.053.00000421
TCGTACC550.053.00000450
ACGTCAG550.053.00000417
CCGGTCA550.053.00000432
TAGCTAG550.053.00000422
TGTCGCA550.053.00000446
TAGTCGA550.053.00000428
AGCGTTG150.006696497653.032
AACCGTT401.6007107E-953.040
AACGTAA850.053.027
TACCCTA203.138425E-453.033
TCGTTAC700.053.046
CAAACGA150.006696497653.040
ACACGGT203.138425E-453.043
TCCGCTA203.138425E-453.050