FastQCFastQC Report
Fri 17 Jun 2016
SRR1527984_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527984_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1820232
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGCG1050.046.00000414
TCACGGT501.6370905E-1146.043
CTATGCG206.312431E-446.018
TAATACG301.8620067E-646.044
ACCGGTG650.046.035
GCCCAAT206.312431E-446.019
CGTATTC253.417947E-546.031
GATCGTT301.8620067E-646.028
GTATTCG206.312431E-446.044
TACTTGA206.312431E-446.040
CACGGTA206.312431E-446.025
CGAAAAC405.6152203E-946.019
GCGAACT850.046.045
CTATAGC301.8620067E-646.028
CGGTTCA405.6152203E-946.045
GACGTAG206.312431E-446.019
ATTACAT253.417947E-546.033
TCTTACG206.312431E-446.017
ACCGATT301.8620067E-646.041
ACCGATG253.417947E-546.035
Produced by FastQC (version 0.11.5)