##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527984_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1820232
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.390222784787873	31.0	31.0	33.0	30.0	34.0
2	32.11483261474361	33.0	31.0	34.0	30.0	34.0
3	32.33282295883162	34.0	31.0	34.0	30.0	34.0
4	35.945093812217344	37.0	35.0	37.0	35.0	37.0
5	35.674733770200724	37.0	35.0	37.0	33.0	37.0
6	35.95068320961284	37.0	35.0	37.0	35.0	37.0
7	34.91151292802236	35.0	35.0	37.0	33.0	37.0
8	35.64581932413011	36.0	35.0	37.0	35.0	37.0
9	38.1367215827433	39.0	39.0	39.0	36.0	39.0
10	37.64318174826066	39.0	37.0	39.0	35.0	39.0
11	37.91300284798861	39.0	37.0	39.0	35.0	39.0
12	38.005590496156536	39.0	38.0	39.0	35.0	39.0
13	38.05073858716911	39.0	38.0	39.0	37.0	39.0
14	38.6896038526957	40.0	39.0	40.0	36.0	40.0
15	38.17130783328719	40.0	38.0	40.0	34.0	40.0
16	37.62386003542406	40.0	37.0	40.0	33.0	40.0
17	37.59597293092309	40.0	37.0	40.0	33.0	40.0
18	37.57702644498064	40.0	37.0	40.0	33.0	40.0
19	37.750743861222084	40.0	37.0	40.0	33.0	40.0
20	37.75434614928207	40.0	37.0	40.0	33.0	40.0
21	37.56401107111621	40.0	37.0	40.0	33.0	40.0
22	37.63709021707123	40.0	37.0	40.0	33.0	40.0
23	37.5631122845879	40.0	37.0	40.0	33.0	40.0
24	37.58376789332349	40.0	37.0	40.0	33.0	40.0
25	37.694394450817256	40.0	37.0	40.0	33.0	40.0
26	37.663890646906545	40.0	37.0	40.0	33.0	40.0
27	37.6427114785368	40.0	37.0	40.0	33.0	40.0
28	37.61004531290517	40.0	37.0	40.0	33.0	40.0
29	37.58059686897055	40.0	37.0	40.0	33.0	40.0
30	37.5169731111199	40.0	36.0	40.0	33.0	40.0
31	37.46710748959473	40.0	36.0	40.0	33.0	40.0
32	37.41025209973234	40.0	36.0	40.0	33.0	40.0
33	37.331112737277444	40.0	36.0	40.0	33.0	40.0
34	37.23490577025346	40.0	36.0	40.0	33.0	40.0
35	37.157710665453635	40.0	35.0	40.0	33.0	40.0
36	37.04735550193602	40.0	35.0	40.0	33.0	40.0
37	36.92018709702939	40.0	35.0	40.0	32.0	40.0
38	36.71166862246131	39.0	35.0	40.0	31.0	40.0
39	36.56176135789284	39.0	35.0	40.0	31.0	40.0
40	36.397553718427105	39.0	35.0	40.0	31.0	40.0
41	36.21671742942658	39.0	35.0	40.0	31.0	40.0
42	36.01920854044979	39.0	35.0	40.0	30.0	40.0
43	35.82031850884942	38.0	35.0	40.0	30.0	40.0
44	35.66719572010601	38.0	35.0	40.0	29.0	40.0
45	35.463589256754084	38.0	35.0	40.0	29.0	40.0
46	35.299430512154494	38.0	35.0	40.0	28.0	40.0
47	35.13970636710046	37.0	35.0	40.0	28.0	40.0
48	34.969474770249064	37.0	34.0	40.0	27.0	40.0
49	34.808993029459984	37.0	34.0	40.0	27.0	40.0
50	34.637507746265314	37.0	34.0	40.0	26.0	40.0
51	34.44603435166506	36.0	34.0	40.0	26.0	40.0
52	33.83276197759407	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	10.0
16	46.0
17	151.0
18	310.0
19	651.0
20	1162.0
21	1834.0
22	3063.0
23	4467.0
24	6449.0
25	8973.0
26	12699.0
27	16977.0
28	22700.0
29	29118.0
30	36710.0
31	43492.0
32	51224.0
33	62486.0
34	90543.0
35	155108.0
36	156315.0
37	288254.0
38	589820.0
39	237666.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.12658386403491	17.67505460842354	31.674149229329007	16.524212298212536
2	32.07310936188354	19.49883311577865	30.873866627990274	17.554190894347535
3	20.715656026264785	23.4258600002637	36.89480242079032	18.963681552681198
4	18.97098831357761	22.285236167697303	34.80083857442348	23.942936944301604
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.34129275828576	0.5416342532160736	0.06889231702332449	0.04818067147484496
12	99.38672652716797	0.46032593647403186	0.08213238751983264	0.07081514883817008
13	97.82747473948376	1.261322732486848	0.31616848841246614	0.595034039616928
14	61.775092405803214	22.82209081040219	4.84668987249977	10.556126911294824
15	32.237758703286175	25.27512976367848	17.410967393167464	25.076144139867885
16	27.900839013927897	17.60561291088169	30.827334098071013	23.666213977119398
17	22.182117444369727	17.763889438269405	29.139692083206974	30.91430103415389
18	20.52342778283208	20.58622197609975	34.093621032923274	24.796729208144896
19	23.297195082824608	20.057003722602392	31.12833968417213	25.517461510400874
20	27.15615372106413	20.037830342505792	27.314869752866667	25.491146183563412
21	29.240887974719705	20.69401043383481	23.145840749970333	26.91926084147515
22	28.374020454535465	22.294960202875238	23.5515033248509	25.779516017738395
23	27.14895683627142	22.44790773923324	22.9852568244048	27.41787860009054
24	27.069571351344223	24.209331557735496	21.980879360433175	26.740217730487103
25	28.341332313683093	22.62799467320649	23.934146856005167	25.09652615710525
26	28.0619173819601	22.37929011246918	21.992581165477805	27.56621134009291
27	29.540849737835618	24.294595414210935	21.37304475473456	24.791510093218886
28	29.263192823771917	23.484643715746127	20.005636644120088	27.24652681636187
29	28.680739598029266	23.382019434885223	19.475044939326416	28.462196027759102
30	30.01403117844319	22.250240628667115	20.114963367307023	27.620764825582672
31	27.2582286214065	22.83214447389124	19.85796316073995	30.051663743962308
32	27.88342365149058	24.704762909343426	20.135675012855504	27.276138426310492
33	29.795377732069316	25.181735075528834	19.74468089781962	25.27820629458223
34	27.34003138061522	23.58556491699959	21.02962699260314	28.04477670978205
35	28.697275951636936	25.073507113378955	20.605230542040793	25.623986392943316
36	32.24940557027895	23.610946296955554	19.011477657793073	25.12817047497242
37	30.282568375899338	24.07517283511113	20.7209850172945	24.921273771695038
38	29.038496191694247	25.53223984634926	22.110258472546356	23.31900548941014
39	29.225175691889827	25.75759573504916	23.03437144276114	21.982857130299873
40	29.51189738450923	26.87371719648924	21.345301038548932	22.269084380452604
41	30.124017158252354	26.34367487221409	20.084033244113936	23.448274725419616
42	31.56982186886067	24.101268409741174	20.09760294292156	24.23130677847659
43	29.149745746696027	24.606094168215918	20.663135248693575	25.581024836394484
44	29.9731023298129	25.707492231759467	20.939583525616516	23.379821912811114
45	26.980187140979833	24.727891829173423	22.191731603443955	26.100189426402785
46	27.166317260656886	26.138536186595996	20.659564275323145	26.035582277423973
47	30.195876130075728	22.024115607241274	21.826942939141823	25.953065323541175
48	29.083600332265334	21.495666486469855	21.54258358275209	27.87814959851272
49	28.607946679324392	22.629697752813925	21.48830478752159	27.274050780340087
50	29.33252464521006	23.03777760197601	21.544890980929903	26.084806771884022
51	28.601299175050215	22.950920541996844	22.442743562359084	26.00503672059386
52	28.530703778419454	23.35257263909216	21.721352003480877	26.39537157900751
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	8.0
4	15.0
5	17.0
6	19.0
7	51.0
8	83.0
9	225.5
10	368.0
11	571.5
12	775.0
13	1071.5
14	1617.0
15	1866.0
16	2266.0
17	2666.0
18	3657.5
19	4649.0
20	6075.0
21	7501.0
22	9597.5
23	11694.0
24	14014.5
25	16335.0
26	17066.5
27	17798.0
28	18527.0
29	19256.0
30	19457.5
31	19659.0
32	21120.5
33	22582.0
34	23398.5
35	24215.0
36	28125.5
37	32036.0
38	35954.5
39	46261.0
40	52649.0
41	56493.5
42	60338.0
43	65011.5
44	69685.0
45	78346.5
46	87008.0
47	91418.0
48	95828.0
49	104766.0
50	113704.0
51	122383.0
52	131062.0
53	137960.5
54	144859.0
55	152699.0
56	160539.0
57	154767.0
58	148995.0
59	146466.0
60	143937.0
61	132709.0
62	121481.0
63	100779.0
64	73008.0
65	65939.0
66	56079.5
67	46220.0
68	37651.5
69	29083.0
70	24431.5
71	19780.0
72	16273.5
73	12767.0
74	10205.5
75	7644.0
76	5527.5
77	3411.0
78	2567.0
79	1723.0
80	1102.0
81	481.0
82	338.0
83	195.0
84	112.0
85	29.0
86	34.0
87	39.0
88	19.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1820232.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.907697248848443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.18962600368906	8.637764656977764
2	6.674991907533384	1.4561758173177504
3	3.097080438581125	1.01346047338121
4	1.8198938259295718	0.7940340351315304
5	1.2330894212010068	0.6725083043609169
6	0.8269161499651361	0.5411850608401837
7	0.578642512053607	0.44181601357557165
8	0.41634627097747196	0.36331032596074236
9	0.31794743256989166	0.31212669019689293
>10	2.423893896476987	5.498508919891038
>50	0.7923640747631935	6.468317564319472
>100	2.4278197578850382	58.84802281701242
>500	0.18667732363177894	13.06445218230462
>1k	0.01471098474272171	1.8883171387298707
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.024667185281876156	0.0	0.0	0.0	0.0
11	0.02675483125227993	0.0	0.0	0.0	0.0
12	0.033292459422754904	0.0	0.0	0.0	0.0
13	0.04587327329703027	0.0	0.0	0.0	0.0
14	0.08696693608287295	0.0	0.0	0.0	0.0
15	0.13740006768367988	0.0	0.0	0.0	0.0
16	0.16673698737303816	0.0	0.0	0.0	0.0
17	0.1910196062919452	0.0	0.0	0.0	0.0
18	0.22249911000355999	0.0	0.0	0.0	0.0
19	0.2526051624188565	0.0	0.0	0.0	0.0
20	0.2797445600341055	0.0	0.0	0.0	0.0
21	0.3103450549160766	0.0	0.0	0.0	0.0
22	0.34259369135362966	0.0	0.0	0.0	0.0
23	0.3725898676652207	0.0	0.0	0.0	0.0
24	0.4034101147546027	0.0	0.0	0.0	0.0
25	0.4395044148218469	0.0	0.0	0.0	0.0
26	0.48499312175590803	0.0	0.0	0.0	0.0
27	0.5304818286899692	0.0	0.0	0.0	0.0
28	0.5777834913351705	0.0	0.0	0.0	0.0
29	0.6295351361804429	0.0	0.0	0.0	0.0
30	0.6926589577592307	0.0	0.0	0.0	0.0
31	0.7479815759749306	0.0	0.0	0.0	0.0
32	0.7926462121312009	0.0	0.0	0.0	0.0
33	0.8323664236207252	0.0	0.0	0.0	0.0
34	0.8649446883693946	0.0	0.0	0.0	0.0
35	0.8982371477921496	0.0	0.0	0.0	0.0
36	0.9252117312518404	0.0	0.0	0.0	0.0
37	0.9519665625041204	0.0	0.0	0.0	0.0
38	0.978391765445284	0.0	0.0	0.0	0.0
39	1.0098712691568987	0.0	0.0	0.0	0.0
40	1.0460754453278482	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAAGCG	105	0.0	46.000004	14
TCACGGT	50	1.6370905E-11	46.0	43
CTATGCG	20	6.312431E-4	46.0	18
TAATACG	30	1.8620067E-6	46.0	44
ACCGGTG	65	0.0	46.0	35
GCCCAAT	20	6.312431E-4	46.0	19
CGTATTC	25	3.417947E-5	46.0	31
GATCGTT	30	1.8620067E-6	46.0	28
GTATTCG	20	6.312431E-4	46.0	44
TACTTGA	20	6.312431E-4	46.0	40
CACGGTA	20	6.312431E-4	46.0	25
CGAAAAC	40	5.6152203E-9	46.0	19
GCGAACT	85	0.0	46.0	45
CTATAGC	30	1.8620067E-6	46.0	28
CGGTTCA	40	5.6152203E-9	46.0	45
GACGTAG	20	6.312431E-4	46.0	19
ATTACAT	25	3.417947E-5	46.0	33
TCTTACG	20	6.312431E-4	46.0	17
ACCGATT	30	1.8620067E-6	46.0	41
ACCGATG	25	3.417947E-5	46.0	35
>>END_MODULE