FastQCFastQC Report
Fri 17 Jun 2016
SRR1527983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1806831
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTT405.6152203E-946.00000440
CTAGACG206.312417E-446.00000439
CAAACCG351.0199983E-746.00000438
CGAACGC206.312417E-446.00000436
TTTACGT405.6152203E-946.00000441
TATGGTA800.046.00000426
TAGCGAC1450.046.00000434
CGACCTT206.312417E-446.00000438
CGACCGA206.312417E-446.00000436
CGATTTA206.312417E-446.00000417
ATAGACC351.0199983E-746.00000415
TTATCGC351.0199983E-746.00000425
CGTTCAC206.312417E-446.00000435
CCATGCG351.0199983E-746.00000442
ACGATTT405.6152203E-946.00000435
GTTACCG206.312417E-446.00000446
ACGATAG405.6152203E-946.00000430
TACGATT405.6152203E-946.00000434
CGCTATA206.312417E-446.00000426
TACGACA206.312417E-446.00000446