##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527983_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1806831
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34339182801269	31.0	31.0	33.0	30.0	34.0
2	31.956966644915877	33.0	31.0	34.0	30.0	34.0
3	32.25927328012415	33.0	31.0	34.0	30.0	34.0
4	35.91267639308823	37.0	35.0	37.0	35.0	37.0
5	35.65383536146989	37.0	35.0	37.0	33.0	37.0
6	35.944532167092554	37.0	35.0	37.0	35.0	37.0
7	34.86898497977951	35.0	35.0	37.0	33.0	37.0
8	35.569329948401375	36.0	35.0	37.0	35.0	37.0
9	38.12264345696969	39.0	39.0	39.0	35.0	39.0
10	37.63224950202869	39.0	37.0	39.0	35.0	39.0
11	37.898425475321154	39.0	37.0	39.0	35.0	39.0
12	37.96776344882283	39.0	37.0	39.0	35.0	39.0
13	38.05843158546649	39.0	38.0	39.0	37.0	39.0
14	38.712497737751896	40.0	39.0	40.0	36.0	40.0
15	38.193229471931794	40.0	38.0	40.0	34.0	40.0
16	37.652424604182684	40.0	37.0	40.0	33.0	40.0
17	37.635404196629345	40.0	37.0	40.0	33.0	40.0
18	37.64505922247294	40.0	37.0	40.0	33.0	40.0
19	37.80824437924742	40.0	37.0	40.0	33.0	40.0
20	37.79249083063109	40.0	37.0	40.0	33.0	40.0
21	37.787736650522376	40.0	37.0	40.0	33.0	40.0
22	37.753977544109	40.0	37.0	40.0	33.0	40.0
23	37.65096126865213	40.0	37.0	40.0	33.0	40.0
24	37.66988390170415	40.0	37.0	40.0	33.0	40.0
25	37.60754215529842	40.0	37.0	40.0	33.0	40.0
26	37.611827005403384	40.0	37.0	40.0	33.0	40.0
27	37.611759483869825	40.0	37.0	40.0	33.0	40.0
28	37.42694529814908	40.0	37.0	40.0	33.0	40.0
29	37.50198773432601	40.0	37.0	40.0	33.0	40.0
30	37.50151840432226	40.0	36.0	40.0	33.0	40.0
31	37.49115274201073	40.0	36.0	40.0	33.0	40.0
32	37.433219819673226	40.0	36.0	40.0	33.0	40.0
33	37.34574511949374	40.0	36.0	40.0	33.0	40.0
34	37.27345556944728	40.0	36.0	40.0	33.0	40.0
35	37.129926927310855	40.0	35.0	40.0	33.0	40.0
36	36.651870042079196	40.0	35.0	40.0	31.0	40.0
37	36.74162497765425	39.0	35.0	40.0	31.0	40.0
38	36.640306149274615	39.0	35.0	40.0	31.0	40.0
39	36.53078898911962	39.0	35.0	40.0	31.0	40.0
40	36.375794969202985	39.0	35.0	40.0	31.0	40.0
41	36.22617887339768	39.0	35.0	40.0	31.0	40.0
42	35.8898900893332	39.0	35.0	40.0	30.0	40.0
43	35.69340685432118	38.0	35.0	40.0	29.0	40.0
44	35.55279768832835	38.0	35.0	40.0	29.0	40.0
45	35.1174869149356	38.0	35.0	40.0	27.0	40.0
46	35.10613333510439	38.0	35.0	40.0	27.0	40.0
47	35.02423912363691	37.0	34.0	40.0	27.0	40.0
48	34.85182288769675	37.0	34.0	40.0	26.0	40.0
49	34.701212786364636	37.0	34.0	40.0	26.0	40.0
50	34.466344666435326	37.0	34.0	40.0	25.0	40.0
51	34.364921788479386	36.0	34.0	40.0	25.0	40.0
52	33.881774222381615	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	8.0
16	40.0
17	120.0
18	354.0
19	631.0
20	1098.0
21	1941.0
22	2919.0
23	4573.0
24	6344.0
25	9386.0
26	12983.0
27	17526.0
28	22646.0
29	29394.0
30	36592.0
31	44437.0
32	51519.0
33	64063.0
34	92662.0
35	155045.0
36	157947.0
37	290711.0
38	570609.0
39	233279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.18476880239491	17.676030575078688	31.579821245041735	16.559379377484667
2	32.173235903081135	19.542890286916705	30.782070929710635	17.501802880291518
3	20.740179906145066	23.35697140463054	36.986248298817095	18.916600390407293
4	18.997183466522323	22.301034241719343	34.82683217190761	23.87495011985072
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.32146393326215	0.5574400704880534	0.0740523048364789	0.04704369141330872
12	99.38450247975598	0.4585929730007953	0.08644970116186848	0.07045484608134352
13	97.8189437750404	1.2565646704091307	0.32454612523252035	0.5999454293179606
14	61.76178070887648	22.8098256007341	4.890440777250335	10.537952913139081
15	32.28979356674753	25.227151847627145	17.4473428892907	25.035711696334634
16	27.899012137825842	17.59378713338436	30.831881897089435	23.675318831700366
17	22.179606172353694	17.750359607511715	29.14495046852749	30.925083751607097
18	20.466828386274088	20.642329028005385	34.11398188319771	24.776860702522814
19	23.24600363841444	20.06867272035957	31.200372364653916	25.484951276572076
20	27.179575732318074	20.083837392650448	27.270287038466794	25.466299836564684
21	28.885656710561197	20.803273798158212	23.195251797207376	27.115817694073215
22	28.335300866544795	22.312656800774395	23.569000089106286	25.78304224357452
23	27.067113637080613	22.461646938756306	23.020747374823657	27.450492049339424
24	27.012044845367388	24.235968942308382	21.95479267291739	26.797193539406837
25	28.355059216938383	22.691330843891873	23.93228807785565	25.02132186131409
26	28.000792547836518	22.373813599611697	22.005710550682384	27.619683301869408
27	29.533254632004873	24.275596334134182	21.3784797803447	24.81266925351624
28	29.420349772612937	23.45703610354261	20.01399134728151	27.108622776562946
29	28.659127500026287	23.411818814266525	19.47542409887809	28.453629586829095
30	30.01215941059236	22.26478292657144	20.10752527491503	27.61553238792117
31	27.300616383048553	22.85260768716056	19.886475270791788	29.9603006589991
32	27.91395542803948	24.653384848942707	20.18130085215496	27.251358870862852
33	29.77649818937134	25.172802547664947	19.788845774729346	25.26185348823437
34	27.30083776512579	23.64731399892962	21.056590240039053	27.995257995905536
35	28.728641472279364	25.058901468925427	20.614656268350497	25.59780079044471
36	32.386426843462395	23.441816085732423	19.0870645898814	25.08469248092378
37	30.264867051760792	24.06002553642261	20.736139683235454	24.938967728581147
38	29.039517254242377	25.51356491005523	22.132341098863147	23.31457673683925
39	29.241362363165123	25.68347565433624	23.028495747527025	22.046666234971614
40	29.519030833542264	26.92609325387931	21.349700110303623	22.205175802274812
41	30.16430424317493	26.294766915112707	20.150971507573203	23.389957334139165
42	31.70086189577221	24.032076049171174	20.15423689321248	24.112825161844135
43	29.10952933616924	24.595770163341232	20.711344890584677	25.583355609904856
44	30.12152215674847	25.653644419428268	20.88269461836774	23.342138805455516
45	27.027818318370674	24.72206863840614	22.206282712661007	26.043830330562184
46	27.145593583461874	26.170737606339493	20.67160680772026	26.01206200247837
47	30.177697858847896	21.96979130865034	21.853897791215672	25.998613041286095
48	29.1376448599786	21.483691612552587	21.570805459946172	27.807858067522638
49	28.639590531709942	22.571618485624832	21.530845995004512	27.257944987660714
50	29.330800722369716	23.014382640103033	21.601743605240333	26.053073032286918
51	28.74225647002957	22.880778556489233	22.41189131689682	25.965073656584376
52	28.501392769993426	23.396820178533577	21.665667680043125	26.436119371429868
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	7.0
4	14.0
5	18.5
6	23.0
7	49.0
8	75.0
9	239.0
10	403.0
11	590.0
12	777.0
13	1042.0
14	1583.5
15	1860.0
16	2260.0
17	2660.0
18	3560.0
19	4460.0
20	5991.0
21	7522.0
22	9536.5
23	11551.0
24	13859.0
25	16167.0
26	16799.5
27	17432.0
28	18143.0
29	18854.0
30	19013.5
31	19173.0
32	20895.5
33	22618.0
34	23601.5
35	24585.0
36	28207.5
37	31830.0
38	35510.0
39	45730.0
40	52270.0
41	56364.0
42	60458.0
43	65042.0
44	69626.0
45	77842.0
46	86058.0
47	90929.5
48	95801.0
49	104380.5
50	112960.0
51	121432.5
52	129905.0
53	137187.5
54	144470.0
55	151731.0
56	158992.0
57	153572.5
58	148153.0
59	145475.0
60	142797.0
61	131314.0
62	119831.0
63	99970.5
64	72678.5
65	65247.0
66	55507.0
67	45767.0
68	37164.5
69	28562.0
70	24076.5
71	19591.0
72	16027.0
73	12463.0
74	10005.5
75	7548.0
76	5402.0
77	3256.0
78	2494.0
79	1732.0
80	1096.5
81	461.0
82	345.0
83	229.0
84	126.0
85	23.0
86	21.5
87	20.0
88	10.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1806831.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.15235234046724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.55890099757808	9.668277967429193
2	7.124316981007202	1.7315442027674675
3	3.1146226363975016	1.1354997505529227
4	1.788329308209018	0.8692963141656008
5	1.1429618623015594	0.6944837631202575
6	0.7734259408157916	0.5639366725230518
7	0.5218164052607249	0.44389077696350604
8	0.40318103493537527	0.391967839482313
9	0.2763672657705613	0.3022661150113871
>10	2.176100333228613	5.527387595708625
>50	0.7459083582102815	6.795160416853201
>100	2.2166409815879087	58.96717485417473
>500	0.14712011587795462	11.459470398895476
>1k	0.010307778819474527	1.4496433323522515
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.027506723096958154	0.0	0.0	0.0	0.0
11	0.029277779714870954	0.0	0.0	0.0	0.0
12	0.03636200618652215	0.0	0.0	0.0	0.0
13	0.048814748031221517	0.0	0.0	0.0	0.0
14	0.09037923303286251	0.0	0.0	0.0	0.0
15	0.13864052587098627	0.0	0.0	0.0	0.0
16	0.16636863104518354	0.0	0.0	0.0	0.0
17	0.19027789538700632	0.0	0.0	0.0	0.0
18	0.22271036970253444	0.0	0.0	0.0	0.0
19	0.2537038605160084	0.0	0.0	0.0	0.0
20	0.2805464373812493	0.0	0.0	0.0	0.0
21	0.30860661567130515	0.0	0.0	0.0	0.0
22	0.34020890719718666	0.0	0.0	0.0	0.0
23	0.37230930839685616	0.0	0.0	0.0	0.0
24	0.4031367626524008	0.0	0.0	0.0	0.0
25	0.4380597853368688	0.0	0.0	0.0	0.0
26	0.4802884165702271	0.0	0.0	0.0	0.0
27	0.5262805431166501	0.0	0.0	0.0	0.0
28	0.5711657592768776	0.0	0.0	0.0	0.0
29	0.623190547428066	0.0	0.0	0.0	0.0
30	0.6834618179564109	0.0	0.0	0.0	0.0
31	0.7391394103820446	0.0	0.0	0.0	0.0
32	0.7846334272546795	0.0	0.0	0.0	0.0
33	0.8218809617501581	0.0	0.0	0.0	0.0
34	0.8542580905463765	0.0	0.0	0.0	0.0
35	0.8850855448019211	0.0	0.0	0.0	0.0
36	0.9111532843968252	0.0	0.0	0.0	0.0
37	0.9396008813220494	0.0	0.0	0.0	0.0
38	0.9663327671486708	0.0	0.0	0.0	0.0
39	0.9952231282283733	0.0	0.0	0.0	0.0
40	1.0281537122176894	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTT	40	5.6152203E-9	46.000004	40
CTAGACG	20	6.312417E-4	46.000004	39
CAAACCG	35	1.0199983E-7	46.000004	38
CGAACGC	20	6.312417E-4	46.000004	36
TTTACGT	40	5.6152203E-9	46.000004	41
TATGGTA	80	0.0	46.000004	26
TAGCGAC	145	0.0	46.000004	34
CGACCTT	20	6.312417E-4	46.000004	38
CGACCGA	20	6.312417E-4	46.000004	36
CGATTTA	20	6.312417E-4	46.000004	17
ATAGACC	35	1.0199983E-7	46.000004	15
TTATCGC	35	1.0199983E-7	46.000004	25
CGTTCAC	20	6.312417E-4	46.000004	35
CCATGCG	35	1.0199983E-7	46.000004	42
ACGATTT	40	5.6152203E-9	46.000004	35
GTTACCG	20	6.312417E-4	46.000004	46
ACGATAG	40	5.6152203E-9	46.000004	30
TACGATT	40	5.6152203E-9	46.000004	34
CGCTATA	20	6.312417E-4	46.000004	26
TACGACA	20	6.312417E-4	46.000004	46
>>END_MODULE