FastQCFastQC Report
Fri 17 Jun 2016
SRR1527982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803764
Sequences flagged as poor quality0
Sequence length59
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA203.1387238E-453.00000425
TTTACGT401.6025297E-953.00000441
TAAACCG203.1387238E-453.00000443
TCGATAT800.053.00000452
CGTTATT203.1387238E-453.00000443
TACGATT401.6025297E-953.00000434
CGAGCAT203.1387238E-453.00000436
ACGTCTA203.1387238E-453.00000447
CCGACTA203.1387238E-453.00000434
CGATGTA203.1387238E-453.00000435
TGCTACG1300.053.00000432
CAATGCG401.6025297E-953.00000415
ATACGTC203.1387238E-453.00000446
AACTATC203.1387238E-453.00000431
TATACGA650.053.00000433
TGTCGAT203.1387238E-453.00000440
CGATACC203.1387238E-453.00000434
AATACGT203.1387238E-453.00000445
TACGTTC401.6025297E-953.00000443
TACGTCT203.1387238E-453.00000447