##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527982_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1803764
Sequences flagged as poor quality	0
Sequence length	59
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.018639356368126	31.0	31.0	33.0	30.0	33.0
2	31.624702012014875	31.0	31.0	34.0	30.0	34.0
3	31.972276306656525	31.0	31.0	34.0	30.0	34.0
4	35.7372949011068	37.0	35.0	37.0	35.0	37.0
5	35.20894972956551	37.0	35.0	37.0	33.0	37.0
6	35.61706243167066	37.0	35.0	37.0	33.0	37.0
7	34.28281859489379	35.0	35.0	36.0	32.0	37.0
8	35.2575347994527	35.0	35.0	37.0	33.0	37.0
9	37.86266717818961	39.0	37.0	39.0	35.0	39.0
10	37.20984507951151	39.0	37.0	39.0	33.0	39.0
11	37.35046491669642	39.0	37.0	39.0	35.0	39.0
12	37.638388946669295	39.0	37.0	39.0	35.0	39.0
13	37.69038299910631	39.0	37.0	39.0	35.0	39.0
14	38.08811075063035	40.0	38.0	40.0	34.0	40.0
15	37.52357902696805	40.0	37.0	40.0	32.0	40.0
16	36.97879101700666	39.0	36.0	40.0	32.0	40.0
17	36.88676345686021	39.0	36.0	40.0	32.0	40.0
18	36.79492051066548	39.0	36.0	40.0	31.0	40.0
19	37.07158918794255	39.0	36.0	40.0	32.0	40.0
20	37.166940353616106	39.0	36.0	40.0	32.0	40.0
21	37.147885754455686	39.0	36.0	40.0	32.0	40.0
22	37.12810489620593	39.0	36.0	40.0	32.0	40.0
23	37.05453817683466	39.0	36.0	40.0	32.0	40.0
24	37.050840908234115	39.0	36.0	40.0	32.0	40.0
25	36.964515313533255	39.0	36.0	40.0	31.0	40.0
26	36.79325011476002	39.0	35.0	40.0	31.0	40.0
27	36.675761906768294	39.0	35.0	40.0	31.0	40.0
28	36.6598618222783	39.0	35.0	40.0	31.0	40.0
29	36.580324255279514	39.0	35.0	40.0	30.0	40.0
30	36.522809524971116	39.0	35.0	40.0	30.0	40.0
31	36.499705061194256	39.0	35.0	40.0	30.0	40.0
32	36.37712527802972	39.0	35.0	40.0	30.0	40.0
33	36.228548191448546	39.0	35.0	40.0	30.0	40.0
34	36.1415905850211	38.0	35.0	40.0	30.0	40.0
35	36.02775695711856	38.0	35.0	40.0	30.0	40.0
36	35.81793737983461	38.0	35.0	40.0	29.0	40.0
37	35.6679715306437	38.0	35.0	40.0	29.0	40.0
38	35.43527146566846	38.0	34.0	40.0	27.0	40.0
39	35.25072126952306	38.0	34.0	40.0	27.0	40.0
40	35.045111222975954	38.0	34.0	40.0	26.0	40.0
41	34.86895624926542	38.0	34.0	40.0	26.0	40.0
42	34.70341851816535	37.0	33.0	40.0	25.0	40.0
43	34.46978540429901	37.0	33.0	40.0	24.0	40.0
44	34.329239301815534	37.0	33.0	40.0	24.0	40.0
45	34.07789988047217	37.0	33.0	40.0	23.0	40.0
46	33.85023317906334	36.0	33.0	40.0	23.0	40.0
47	33.731866807409396	36.0	33.0	40.0	23.0	40.0
48	33.531984782931694	36.0	33.0	39.0	22.0	40.0
49	33.387891098835546	36.0	32.0	39.0	22.0	40.0
50	33.16137587844086	35.0	32.0	39.0	20.0	40.0
51	32.967752433245145	35.0	32.0	39.0	20.0	40.0
52	32.807722628902674	35.0	32.0	39.0	20.0	40.0
53	32.52384181079121	35.0	31.0	38.0	20.0	40.0
54	32.36029436223364	35.0	31.0	38.0	20.0	40.0
55	32.17321002082312	35.0	31.0	38.0	18.0	40.0
56	31.966816612372792	35.0	31.0	38.0	18.0	40.0
57	31.79095879505301	35.0	31.0	37.0	18.0	40.0
58	31.561309018252942	35.0	30.0	37.0	17.0	40.0
59	31.07103756367241	34.0	29.0	36.0	15.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	3.0
14	12.0
15	70.0
16	266.0
17	645.0
18	1320.0
19	2445.0
20	3979.0
21	6246.0
22	8810.0
23	12511.0
24	16453.0
25	21557.0
26	27465.0
27	33841.0
28	39634.0
29	44166.0
30	50413.0
31	59579.0
32	73428.0
33	92038.0
34	129043.0
35	169089.0
36	203514.0
37	305675.0
38	422921.0
39	78640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.05101776063831	17.964600690555972	31.28358255292821	16.70079899587751
2	31.309029340867212	19.981771451254154	30.825928447402212	17.883270760476424
3	20.7601992278369	23.52480701466489	36.97667765849634	18.738316099001864
4	19.095790801900915	22.614821007626272	34.306982509907066	23.982405680565748
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.28937488496278	0.5840564508439019	0.07412277881141878	0.0524458853819014
12	99.37175816791998	0.468076755052213	0.08332575658456429	0.0768393204432509
13	97.77897773766414	1.2899137581191331	0.32620675432040996	0.604901749896328
14	61.41967574472048	23.05523338973391	4.924092065259091	10.600998800286513
15	32.253554234367684	25.477446051700774	17.453225588269863	24.815774125661672
16	28.015028573582796	17.70070807489228	30.535646570172155	23.748616781352773
17	22.111096573609405	17.964212613179996	29.103419294320098	30.821271518890498
18	20.291235438782458	20.728543201882285	34.050241605886356	24.9299797534489
19	23.194442288459022	20.162504629208698	31.152745037599157	25.490308044733123
20	27.1170175255743	20.230806247380478	27.333786459869476	25.318389767175752
21	28.87611683124843	20.897966696308387	23.250824387225823	26.975092085217355
22	28.290009114274373	22.46735160475539	23.53844516244919	25.704194118521052
23	27.087135567624145	22.593310433072176	23.085946942061156	27.23360705724252
24	27.025985661095355	24.406297054381838	21.942227475434702	26.625489809088105
25	28.314236230460306	22.777758065911062	23.88145012318685	25.02655558044179
26	27.997398772788458	22.50366455922172	22.046897487697947	27.452039180291877
27	29.399799530315494	24.37192448679539	21.518724178994592	24.709551803894524
28	29.284540549650618	23.614896405516465	19.99967845017419	27.100884594658726
29	28.55922393395145	23.48189674480697	19.550506607294523	28.408372713947056
30	29.86538150223643	22.48387261304694	20.229143058626295	27.42160282609033
31	27.199456248156633	23.015926695510057	19.91524390108684	29.869373155246475
32	27.809513883190927	24.878365462444087	20.15080686830428	27.161313786060703
33	29.68891717541763	25.35830629727614	19.769382247344996	25.183394279961234
34	27.25201301278881	23.787424518950374	21.03961493853963	27.920947529721186
35	28.61566147234339	25.264336132664805	20.678204022255684	25.441798372736123
36	32.21812831390359	23.71773691015011	19.100170532286928	24.96396424365937
37	30.11308574735941	24.248959398236135	20.767350939479886	24.87060391492457
38	28.88737107515174	25.800160109637403	22.103612224215585	23.208856590995275
39	29.043211861418676	25.94458033312562	23.083951115556136	21.928256689899566
40	29.368919659112834	27.16491736169477	21.365489055109204	22.100673924083196
41	30.059974586475835	26.520764357199724	20.165054851965113	23.254206204359328
42	31.42750381979017	24.310996338767154	20.12929629375018	24.13220354769249
43	28.941480149287823	24.88984146484795	20.70708806695333	25.461590318910897
44	29.76403786748156	25.938371095110003	21.05718929970883	23.24040173769961
45	26.791586926005838	24.981095087827455	22.19542024344648	26.031897742720222
46	26.89387303438809	26.42651699446269	20.655806413699356	26.023803557449867
47	29.924646461510484	22.24132425306193	21.87414761576348	25.959881669664103
48	28.924238425869458	21.656657966341495	21.520221048873356	27.898882558915687
49	28.458379255822823	22.86258069237439	21.50902224459519	27.170017807207596
50	29.109462213460297	23.293790096708882	21.599998669448997	25.996749020381827
51	28.502287438933255	23.179529029296518	22.37271616464238	25.94546736712785
52	28.288734002896167	23.606691340995827	21.65111400382755	26.45346065228045
53	27.3231420518427	25.338181713350526	21.740593558802594	25.59808267600418
54	26.276774566961087	23.948753828106113	22.96996724626947	26.80450435866333
55	25.473066321314764	24.72845671606707	23.055566027484748	26.742910935133423
56	27.406301489551847	24.290927194466683	22.478716727908974	25.8240545880725
57	27.792826556023957	23.125198196659873	22.762234970872022	26.319740276444147
58	26.85528705529105	23.41287441150838	22.999294807968226	26.73254372523235
59	26.246227333509264	23.828616160428968	22.30485806347172	27.62029844259005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	3.0
4	12.0
5	18.0
6	36.0
7	54.0
8	170.5
9	402.5
10	518.0
11	718.0
12	918.0
13	939.5
14	1018.5
15	1076.0
16	1300.0
17	1524.0
18	1875.0
19	3016.5
20	3807.0
21	4975.0
22	6143.0
23	8095.5
24	10048.0
25	11413.0
26	13855.5
27	14933.0
28	16363.5
29	17794.0
30	18533.5
31	20502.5
32	21732.0
33	24530.0
34	27328.0
35	27835.5
36	29833.5
37	31324.0
38	35232.0
39	39140.0
40	44944.0
41	50748.0
42	52687.0
43	60051.0
44	65476.0
45	64115.5
46	62755.0
47	69617.5
48	81559.0
49	86638.0
50	95322.5
51	104007.0
52	108383.0
53	125711.5
54	138664.0
55	142494.5
56	146325.0
57	141592.0
58	131698.0
59	126537.0
60	113318.0
61	100099.0
62	89077.5
63	78056.0
64	71099.5
65	55065.0
66	45987.0
67	41917.5
68	37848.0
69	34783.0
70	24724.0
71	17730.0
72	15539.5
73	13349.0
74	9137.5
75	4719.5
76	4513.0
77	3048.5
78	1584.0
79	1151.0
80	718.0
81	702.5
82	429.5
83	172.0
84	140.5
85	109.0
86	61.5
87	10.0
88	6.0
89	3.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1803764.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.106320967859215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.4392241407098	13.600052462872783
2	5.827731946887706	1.8772664250244093
3	2.3488705042265807	1.1349498675903187
4	1.3183106631347372	0.8493253870319761
5	0.842391079074592	0.6783910550018327
6	0.5524980489999635	0.5339226546785653
7	0.3885425969199999	0.4380594242975347
8	0.2630675567323768	0.338964040396974
9	0.23517811129643543	0.3409068730639776
>10	1.5151333240007603	5.208238105127956
>50	0.5465347627182949	6.5648845870272
>100	1.614039443135968	56.451897691783
>500	0.10122293596721717	10.586567024040983
>1k	0.007254886195602596	1.396574402062465
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.02528046906358038	0.0	0.0	0.0	0.0
11	0.027220855943460454	0.0	0.0	0.0	0.0
12	0.03287569770768238	0.0	0.0	0.0	0.0
13	0.046125768116006306	0.0	0.0	0.0	0.0
14	0.08382471321081915	0.0	0.0	0.0	0.0
15	0.12906344732459457	0.0	0.0	0.0	0.0
16	0.15218177100773717	0.0	0.0	0.0	0.0
17	0.1739695436875334	0.0	0.0	0.0	0.0
18	0.205071173390754	0.0	0.0	0.0	0.0
19	0.23223658970907501	0.0	0.0	0.0	0.0
20	0.2589584890262806	0.0	0.0	0.0	0.0
21	0.2847933543412553	0.0	0.0	0.0	0.0
22	0.3140654764148747	0.0	0.0	0.0	0.0
23	0.3405101776063831	0.0	0.0	0.0	0.0
24	0.37083565255765166	0.0	0.0	0.0	0.0
25	0.40692684852342104	0.0	0.0	0.0	0.0
26	0.44822936925229684	0.0	0.0	0.0	0.0
27	0.48820133897782636	0.0	0.0	0.0	0.0
28	0.5280069898279376	0.0	0.0	0.0	0.0
29	0.5757405070729874	0.0	0.0	0.0	0.0
30	0.6311801322124181	0.0	0.0	0.0	0.0
31	0.6814638722138816	0.0	0.0	0.0	0.0
32	0.7228772721930363	0.0	0.0	0.0	0.0
33	0.7581368737817142	0.0	0.0	0.0	0.0
34	0.7885177883581223	0.0	0.0	0.0	0.0
35	0.8147961706742124	0.0	0.0	0.0	0.0
36	0.8402429586132111	0.0	0.0	0.0	0.0
37	0.8645809540494211	0.0	0.0	0.0	0.0
38	0.8898614231130014	0.0	0.0	0.0	0.0
39	0.9180801923089716	0.0	0.0	0.0	0.0
40	0.9499579767641443	0.0	0.0	0.0	0.0
41	0.9802834517154129	0.0	0.0	0.0	0.0
42	1.004233369775647	0.0	0.0	0.0	0.0
43	1.0288485633375541	0.0	0.0	0.0	0.0
44	1.053020239898346	0.0	0.0	0.0	0.0
45	1.0768038390831616	0.0	0.0	0.0	0.0
46	1.1023060666472997	0.0	0.0	0.0	0.0
47	1.1257015884561394	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGTA	20	3.1387238E-4	53.000004	25
TTTACGT	40	1.6025297E-9	53.000004	41
TAAACCG	20	3.1387238E-4	53.000004	43
TCGATAT	80	0.0	53.000004	52
CGTTATT	20	3.1387238E-4	53.000004	43
TACGATT	40	1.6025297E-9	53.000004	34
CGAGCAT	20	3.1387238E-4	53.000004	36
ACGTCTA	20	3.1387238E-4	53.000004	47
CCGACTA	20	3.1387238E-4	53.000004	34
CGATGTA	20	3.1387238E-4	53.000004	35
TGCTACG	130	0.0	53.000004	32
CAATGCG	40	1.6025297E-9	53.000004	15
ATACGTC	20	3.1387238E-4	53.000004	46
AACTATC	20	3.1387238E-4	53.000004	31
TATACGA	65	0.0	53.000004	33
TGTCGAT	20	3.1387238E-4	53.000004	40
CGATACC	20	3.1387238E-4	53.000004	34
AATACGT	20	3.1387238E-4	53.000004	45
TACGTTC	40	1.6025297E-9	53.000004	43
TACGTCT	20	3.1387238E-4	53.000004	47
>>END_MODULE