FastQCFastQC Report
Fri 17 Jun 2016
SRR1527981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4358453
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGG351.02045306E-746.00000417
AACCGTT253.4187375E-546.032
AACGTAT253.4187375E-546.029
TAATACG551.8189894E-1246.027
TCGCATA405.6170393E-946.020
CAAACGA206.313403E-446.027
CCTAGTA206.313403E-446.015
CCCTATA405.6170393E-946.030
CGATTTA600.046.033
CAATTCG206.313403E-446.045
ATATGCG405.6170393E-946.029
TCGATCC206.313403E-446.024
ATCGTTA206.313403E-446.022
TAGTTCG301.8626088E-646.035
ATATCGT206.313403E-446.034
ACGGAAT253.4187375E-546.022
GCGATAA206.313403E-446.018
TTACGTA405.6170393E-946.018
CATAACG301.8626088E-646.032
TTACGAT253.4187375E-546.019
Produced by FastQC (version 0.11.5)