FastQCFastQC Report
Fri 17 Jun 2016
SRR1527980_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527980_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4318793
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG453.110472E-1046.027
TCGTTAA501.6370905E-1146.033
CGAAATA253.4187324E-546.026
CGACGTA206.313398E-446.046
ATATCGT253.4187324E-546.034
ATATACG206.313398E-446.014
CCGACTA253.4187324E-546.029
ACGTATA206.313398E-446.035
ATAACGG453.110472E-1046.017
TCATACG301.8626051E-646.028
CGACTAT301.8626051E-646.021
CGACTAA301.8626051E-646.030
GACGAAT501.6370905E-1146.043
CCGCATA405.6170393E-946.045
ATACCCG206.313398E-446.045
AATCGTA453.110472E-1046.039
CGATCTA34850.044.4160652
ACGTCTA43250.044.085552
TACGTCT14300.043.909091
GACATAA1050.043.80952519
Produced by FastQC (version 0.11.5)