##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527980_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4318793 Sequences flagged as poor quality 0 Sequence length 52 %GC 57 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80777268093192 33.0 31.0 34.0 30.0 34.0 2 32.22667328579999 34.0 31.0 34.0 30.0 34.0 3 32.4572893398688 34.0 31.0 34.0 30.0 34.0 4 36.103896389569954 37.0 35.0 37.0 35.0 37.0 5 36.0497453802486 37.0 35.0 37.0 35.0 37.0 6 36.052768447110104 37.0 35.0 37.0 35.0 37.0 7 36.2770656060617 37.0 37.0 37.0 35.0 37.0 8 36.20171654441415 37.0 36.0 37.0 35.0 37.0 9 37.74234120505428 39.0 38.0 39.0 35.0 39.0 10 37.66232255169442 39.0 38.0 39.0 35.0 39.0 11 37.95229894093095 39.0 37.0 39.0 35.0 39.0 12 37.901191837626854 39.0 37.0 39.0 35.0 39.0 13 37.98581409203914 39.0 38.0 39.0 37.0 39.0 14 38.65919482596179 40.0 39.0 40.0 36.0 40.0 15 38.149854137487026 40.0 38.0 40.0 34.0 40.0 16 37.48541687457583 40.0 36.0 40.0 33.0 40.0 17 37.479026663236695 40.0 37.0 40.0 33.0 40.0 18 37.54051814939961 40.0 37.0 40.0 33.0 40.0 19 37.61076277561809 40.0 37.0 40.0 33.0 40.0 20 37.56263034602492 40.0 37.0 40.0 33.0 40.0 21 37.557046146921145 40.0 37.0 40.0 33.0 40.0 22 37.485569695051375 40.0 36.0 40.0 33.0 40.0 23 37.387557588428066 40.0 36.0 40.0 33.0 40.0 24 37.35756610701184 40.0 36.0 40.0 33.0 40.0 25 37.29401038669832 40.0 36.0 40.0 32.0 40.0 26 37.25241265325752 40.0 36.0 40.0 32.0 40.0 27 37.26045494655567 40.0 36.0 40.0 33.0 40.0 28 37.071588288672324 40.0 35.0 40.0 32.0 40.0 29 37.16349776430591 40.0 35.0 40.0 32.0 40.0 30 37.16098548830657 40.0 35.0 40.0 33.0 40.0 31 37.13099863781385 40.0 35.0 40.0 33.0 40.0 32 37.02704737180041 39.0 35.0 40.0 32.0 40.0 33 36.90830817777096 39.0 35.0 40.0 32.0 40.0 34 36.7989067778891 39.0 35.0 40.0 32.0 40.0 35 36.57705891437723 39.0 35.0 40.0 31.0 40.0 36 36.06716992455994 38.0 35.0 40.0 30.0 40.0 37 36.085187227079416 38.0 35.0 40.0 30.0 40.0 38 35.96679697313578 38.0 35.0 40.0 30.0 40.0 39 35.84343565435991 38.0 35.0 40.0 30.0 40.0 40 35.64741977677559 37.0 35.0 40.0 30.0 40.0 41 35.47634234842929 37.0 35.0 40.0 30.0 40.0 42 35.13975941889319 36.0 34.0 40.0 29.0 40.0 43 34.93027311102894 36.0 34.0 40.0 28.0 40.0 44 34.74095887438921 35.0 34.0 40.0 28.0 40.0 45 34.332732548191125 35.0 34.0 39.0 26.0 40.0 46 34.289466756105234 35.0 34.0 39.0 26.0 40.0 47 34.19891622497304 35.0 34.0 39.0 27.0 40.0 48 34.03521863631806 35.0 33.0 39.0 26.0 40.0 49 33.89299278756819 35.0 33.0 38.0 26.0 40.0 50 33.65240589210921 35.0 33.0 38.0 26.0 40.0 51 33.547962127381425 35.0 33.0 37.0 26.0 40.0 52 33.06275572827871 35.0 33.0 37.0 24.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 4.0 13 10.0 14 15.0 15 44.0 16 111.0 17 314.0 18 705.0 19 1614.0 20 2832.0 21 5213.0 22 8180.0 23 12424.0 24 17853.0 25 24887.0 26 33838.0 27 45062.0 28 57719.0 29 73428.0 30 91398.0 31 109053.0 32 130822.0 33 165225.0 34 242766.0 35 453171.0 36 493609.0 37 872689.0 38 1081576.0 39 394230.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.873736944558352 20.403107997998514 34.69893092815516 17.024224129287973 2 24.889731922784907 22.440992193883798 34.614578656582985 18.054697226748306 3 17.22821630951055 22.5837635654221 36.6435946339637 23.54442549110365 4 18.876292519692424 24.55621281223712 36.032822133406256 20.5346725346642 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 0.0 0.0 100.0 0.0 8 0.0 100.0 0.0 0.0 9 0.0 0.0 0.0 100.0 10 0.0 0.0 0.0 100.0 11 98.78155771763083 0.766024210931156 0.15140804386781215 0.30101002757020306 12 98.71929495115882 0.9709657304714535 0.14024751823020923 0.16949180013952972 13 96.21120993759136 2.3639938288313425 0.6489081555888416 0.7758880779884565 14 59.9360747319911 24.769235293286805 5.470741478000914 9.82394849672119 15 34.95087632123142 23.554914532833596 17.448625113544455 24.045584032390533 16 29.95096083558531 16.51845781911752 28.314508243391153 25.216073101906016 17 23.199051216393098 17.153079575705526 29.45786010119031 30.190009106711063 18 23.5292129074026 18.860524225171247 31.591558104312938 26.018704763113217 19 25.86322150656445 18.074888053213016 29.181833905908434 26.8800565343141 20 28.392794005176906 18.575768739089835 26.09391096077075 26.937526294962506 21 31.07326514607206 19.36075658175791 20.982436527983626 28.583541744186398 22 30.40138760991786 20.455321660473192 21.965465814175396 27.177824915433547 23 29.726499973487964 21.026754465888963 21.274393100109222 27.97235246051385 24 28.96010528867672 23.190715554091152 20.892712385150205 26.95646677208192 25 31.19630878349576 20.809193679808224 22.488968561354987 25.50552897534103 26 30.73833360385645 19.463493619629375 21.54407493019462 28.254097846319564 27 31.184986175535617 21.411977837326308 20.301505536384816 27.101530450753252 28 31.800366444976643 21.50524463663806 18.992436081099513 27.701952837285788 29 30.205846865084755 20.00746504868374 19.412136678002394 30.374551408229106 30 29.8372948182513 21.025596735013693 20.112239692895677 29.02486875383932 31 28.091575586049156 20.867126532806736 20.21944557194568 30.821852309198427 32 29.881520137686618 23.01550456342779 18.71124640611393 28.39172889277166 33 30.88057241919212 22.871019750194094 19.62302893424158 26.625378896372204 34 29.41960867307139 22.63150838671823 20.262304768948177 27.686578171262198 35 32.00810967323509 22.518166534029298 19.23224845460294 26.24147533813267 36 33.74310831753224 21.711575433228685 20.467593607751056 24.077722641488027 37 32.24391629791008 21.70599517040988 20.72586021140629 25.324228320273747 38 29.881311746129068 24.61532655072841 22.098674328683963 23.404687374458558 39 31.170329302654697 24.911450954004973 21.929020446221895 21.989199297118432 40 31.18373582619033 25.49906883705702 19.225510460908872 24.091684875843782 41 30.774431652547367 25.611646587368277 18.921745033855526 24.69217672622883 42 32.125781439397535 24.89158429218534 18.646737641743886 24.33589662667324 43 31.81018400279893 23.937197267847754 18.58878163412787 25.66383709522545 44 30.086461657226916 24.904342486430817 18.515288878165727 26.49390697817654 45 26.959060089242527 24.309037270366975 19.13374871173497 29.59815392865553 46 27.623597611647515 23.27550776339593 20.455298505855687 28.64559611910087 47 29.42247984564206 21.005567990871523 21.83561471920511 27.736337444281308 48 29.597111970867786 20.736094552343676 21.27006318663571 28.396730290152828 49 28.966982210075827 21.632733960622794 21.42239741520374 27.97788641409764 50 28.93046737826981 22.64063130601536 20.903386663820193 27.52551465189464 51 28.27491384745692 22.331216152290697 22.405380392160495 26.988489608091893 52 29.157961495260366 22.25980731190404 22.690691589062038 25.891539603773555 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 2.0 6 4.0 7 11.5 8 19.0 9 64.0 10 109.0 11 318.5 12 528.0 13 846.5 14 1401.0 15 1637.0 16 2064.5 17 2492.0 18 3008.5 19 3525.0 20 4280.0 21 5035.0 22 6648.0 23 8261.0 24 10156.5 25 12052.0 26 15563.5 27 19075.0 28 22585.0 29 26095.0 30 28182.0 31 30269.0 32 31565.0 33 32861.0 34 35736.0 35 38611.0 36 42665.5 37 46720.0 38 47756.5 39 50966.5 40 53140.0 41 61117.0 42 69094.0 43 80730.0 44 92366.0 45 102674.5 46 112983.0 47 126330.0 48 139677.0 49 154972.0 50 170267.0 51 187064.5 52 203862.0 53 233528.5 54 263195.0 55 295534.5 56 327874.0 57 354407.0 58 380940.0 59 398882.0 60 416824.0 61 430230.0 62 443636.0 63 410681.0 64 337302.0 65 296878.0 66 254330.5 67 211783.0 68 186923.5 69 162064.0 70 142175.5 71 122287.0 72 101586.5 73 80886.0 74 73186.0 75 65486.0 76 48898.5 77 32311.0 78 22154.0 79 11997.0 80 7989.5 81 3982.0 82 2633.0 83 1284.0 84 892.0 85 500.0 86 480.0 87 460.0 88 243.5 89 19.0 90 11.0 91 6.5 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 4318793.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.405726505219627 #Duplication Level Percentage of deduplicated Percentage of total 1 75.26448233065747 7.079171363590994 2 7.764547752549114 1.460624251943894 3 3.757339753912158 1.0602153033746093 4 2.3602495298416986 0.8879944624705692 5 1.5331936056509443 0.7210399867152169 6 1.0151165629752832 0.5728745257358442 7 0.7288445204725053 0.47987185570746327 8 0.5175414021690653 0.38942823071440874 9 0.3985575225725306 0.3373850748523609 >10 2.5715253716107886 4.898856020696326 >50 0.8926422962899654 6.3096036075307005 >100 2.9786485003677714 62.02503115191097 >500 0.20523802587904824 12.327557772071176 >1k 0.012072825051708721 1.4503463926854525 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.002570162543099426 0.0 0.0 0.0 0.0 12 0.00692323063411467 0.0 0.0 0.0 0.0 13 0.008960836974589891 0.0 0.0 0.0 0.0 14 0.01676394307390977 0.0 0.0 0.0 0.0 15 0.029197972674309697 0.0 0.0 0.0 0.0 16 0.043785381702711845 0.0 0.0 0.0 0.0 17 0.056219411303111776 0.0 0.0 0.0 0.0 18 0.07286758128949454 0.0 0.0 0.0 0.0 19 0.0885201027231451 0.0 0.0 0.0 0.0 20 0.1091971761554675 0.0 0.0 0.0 0.0 21 0.1284849725374659 0.0 0.0 0.0 0.0 22 0.1486063351496587 0.0 0.0 0.0 0.0 23 0.1677088945916139 0.0 0.0 0.0 0.0 24 0.19104874903705735 0.0 0.0 0.0 0.0 25 0.2139718203674036 0.0 0.0 0.0 0.0 26 0.24059963049861385 0.0 0.0 0.0 0.0 27 0.2663938743996297 0.0 0.0 0.0 0.0 28 0.295452919368907 0.0 0.0 0.0 0.0 29 0.3243730366331519 0.0 0.0 0.0 0.0 30 0.35984591065142507 0.0 0.0 0.0 0.0 31 0.3957587224023008 0.0 0.0 0.0 0.0 32 0.42416943808142693 0.0 0.0 0.0 0.0 33 0.4457958508314707 0.0 0.0 0.0 0.0 34 0.46638030579377154 0.0 0.0 0.0 0.0 35 0.48488084518058633 0.0 0.0 0.0 0.0 36 0.5037518584474875 0.0 0.0 0.0 0.0 37 0.5225765624793779 0.0 0.0 0.0 0.0 38 0.5442724390819379 0.0 0.0 0.0 0.0 39 0.5724979178210208 0.0 0.0 0.0 0.0 40 0.6038261153058273 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATACG 45 3.110472E-10 46.0 27 TCGTTAA 50 1.6370905E-11 46.0 33 CGAAATA 25 3.4187324E-5 46.0 26 CGACGTA 20 6.313398E-4 46.0 46 ATATCGT 25 3.4187324E-5 46.0 34 ATATACG 20 6.313398E-4 46.0 14 CCGACTA 25 3.4187324E-5 46.0 29 ACGTATA 20 6.313398E-4 46.0 35 ATAACGG 45 3.110472E-10 46.0 17 TCATACG 30 1.8626051E-6 46.0 28 CGACTAT 30 1.8626051E-6 46.0 21 CGACTAA 30 1.8626051E-6 46.0 30 GACGAAT 50 1.6370905E-11 46.0 43 CCGCATA 40 5.6170393E-9 46.0 45 ATACCCG 20 6.313398E-4 46.0 45 AATCGTA 45 3.110472E-10 46.0 39 CGATCTA 3485 0.0 44.416065 2 ACGTCTA 4325 0.0 44.08555 2 TACGTCT 1430 0.0 43.90909 1 GACATAA 105 0.0 43.809525 19 >>END_MODULE