FastQCFastQC Report
Fri 17 Jun 2016
SRR1527979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4381137
Sequences flagged as poor quality0
Sequence length59
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG307.015042E-753.00000427
AATCACG150.00669751653.00000430
CTAACGT307.015042E-753.00000453
ATATACG307.015042E-753.00000414
ATACGTC307.015042E-753.00000446
CGATATT150.00669751653.00000450
TAATCGC150.00669751653.00000425
CCGATTA950.053.049
CGATTTA401.6025297E-953.033
ATCCGAT353.3449396E-853.040
TTACGTA251.47942155E-553.018
ACGTATA203.1392204E-453.035
TAACTAG203.1392204E-453.040
CGATCTA35750.050.627972
TACGTCT14750.050.484741
ACCGGGG2646300.050.3142478
TTATCTA146500.050.2866942
CGTAATT950.050.2105345
ACGTCTA49300.050.204872
TTTCTAC617750.050.0615163