##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527979_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4381137
Sequences flagged as poor quality	0
Sequence length	59
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.449052608946033	31.0	31.0	34.0	30.0	34.0
2	31.975820660253262	33.0	31.0	34.0	30.0	34.0
3	32.19287641541454	34.0	31.0	34.0	30.0	34.0
4	35.92689705891416	37.0	35.0	37.0	35.0	37.0
5	35.78111344155638	37.0	35.0	37.0	35.0	37.0
6	35.764514554098625	37.0	35.0	37.0	33.0	37.0
7	36.030676968102114	37.0	35.0	37.0	35.0	37.0
8	35.99933396285028	37.0	35.0	37.0	35.0	37.0
9	37.49449332444979	39.0	37.0	39.0	35.0	39.0
10	37.39161295344108	39.0	37.0	39.0	34.0	39.0
11	37.480248848643626	39.0	37.0	39.0	35.0	39.0
12	37.53921482026241	39.0	37.0	39.0	35.0	39.0
13	37.62733007436198	39.0	37.0	39.0	35.0	39.0
14	38.06253331041691	40.0	38.0	40.0	34.0	40.0
15	37.55039228401212	40.0	37.0	40.0	32.0	40.0
16	36.89010866357295	39.0	36.0	40.0	31.0	40.0
17	36.78670171692873	39.0	36.0	40.0	31.0	40.0
18	36.77983340854212	39.0	36.0	40.0	31.0	40.0
19	36.892498454168404	39.0	35.0	40.0	31.0	40.0
20	36.968160548277766	39.0	35.0	40.0	31.0	40.0
21	36.933115535989856	39.0	35.0	40.0	31.0	40.0
22	36.8780024454839	39.0	35.0	40.0	31.0	40.0
23	36.81516419139598	39.0	35.0	40.0	31.0	40.0
24	36.76891432520827	39.0	35.0	40.0	31.0	40.0
25	36.678773341258214	39.0	35.0	40.0	30.0	40.0
26	36.47899985779947	39.0	35.0	40.0	30.0	40.0
27	36.355720444259106	39.0	35.0	40.0	30.0	40.0
28	36.328918725892386	39.0	35.0	40.0	30.0	40.0
29	36.26940198400552	39.0	35.0	40.0	30.0	40.0
30	36.204630898326165	39.0	35.0	40.0	30.0	40.0
31	36.17116150442225	38.0	35.0	40.0	30.0	40.0
32	36.00739328626336	38.0	35.0	40.0	30.0	40.0
33	35.813099658832854	38.0	35.0	40.0	29.0	40.0
34	35.69210184479508	38.0	35.0	40.0	29.0	40.0
35	35.48875759876945	38.0	34.0	40.0	28.0	40.0
36	35.25846121680285	37.0	34.0	40.0	28.0	40.0
37	35.03265704770246	37.0	34.0	40.0	27.0	40.0
38	34.776064067387075	37.0	33.0	40.0	26.0	40.0
39	34.590126718246886	37.0	33.0	40.0	26.0	40.0
40	34.35388256518799	36.0	33.0	39.0	26.0	40.0
41	34.1726757232198	36.0	33.0	39.0	25.0	40.0
42	34.01456699482349	35.0	33.0	39.0	24.0	40.0
43	33.767479537846	35.0	33.0	39.0	24.0	40.0
44	33.58558383360301	35.0	33.0	39.0	23.0	40.0
45	33.36597143618198	35.0	33.0	39.0	23.0	40.0
46	33.11232152749389	35.0	32.0	38.0	23.0	40.0
47	32.96744635011414	35.0	32.0	38.0	23.0	40.0
48	32.771387427510255	35.0	32.0	38.0	23.0	40.0
49	32.66577374777369	35.0	32.0	37.0	23.0	40.0
50	32.4406458871293	35.0	31.0	37.0	22.0	40.0
51	32.24543080939948	35.0	31.0	37.0	22.0	39.0
52	32.05220904984254	35.0	31.0	36.0	20.0	39.0
53	31.83034837760152	35.0	31.0	36.0	20.0	39.0
54	31.657995401650304	34.0	31.0	36.0	20.0	39.0
55	31.536698350222785	34.0	31.0	35.0	20.0	39.0
56	31.322690890515407	34.0	30.0	35.0	20.0	38.0
57	31.178891689531735	34.0	30.0	35.0	20.0	38.0
58	30.997742595129985	34.0	30.0	35.0	18.0	38.0
59	30.508456822966277	34.0	29.0	35.0	18.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	13.0
14	47.0
15	143.0
16	591.0
17	1506.0
18	3263.0
19	6082.0
20	10259.0
21	15881.0
22	22935.0
23	31114.0
24	40834.0
25	53542.0
26	67508.0
27	83301.0
28	97731.0
29	111566.0
30	130334.0
31	155340.0
32	189290.0
33	240693.0
34	345093.0
35	507519.0
36	633654.0
37	822545.0
38	695584.0
39	114764.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.910905319783424	20.701406963534808	34.24451232636642	17.143175390315346
2	24.411425618509533	22.90966933926056	34.56091421016964	18.11799083206026
3	17.18946474396943	22.80239125140346	36.52152397882102	23.486620025806086
4	18.809067143985683	24.820885537247523	35.87680549592492	20.493241822841878
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	100.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	0.0	100.0
11	98.73571632204151	0.7958436360241645	0.14633187686210225	0.32210816507221757
12	98.70150145955262	0.9806586737643677	0.13558124295131604	0.18225862373169338
13	96.18964209519127	2.399057596235863	0.6358851594917028	0.7754151490811632
14	59.64629729679761	25.019715201784376	5.516718605238776	9.817268896179234
15	34.97130995903574	23.678670628195377	17.404523072435307	23.945496340333573
16	30.068222016339597	16.66227283008954	28.132468808895954	25.13703634467491
17	23.28365901363048	17.21900045581775	29.280093272591113	30.21724725796066
18	23.49846626572052	18.986349890450814	31.470255324131614	26.04492851969706
19	25.933245182700286	18.120798322444607	29.149624857656814	26.79633163719829
20	28.393382813639473	18.755724826683117	26.051639106469395	26.799253253208015
21	31.10674238217157	19.495441480145452	20.978549632207347	28.419266505475633
22	30.350021010527634	20.62880024066812	22.01647654478735	27.0047022040169
23	29.69646920422712	21.206595456841455	21.280685812838083	27.81624952609334
24	28.893481304054173	23.37895391082269	20.909709054978194	26.817855730144935
25	31.142737604416386	20.955336480005077	22.551406176067992	25.350519739510542
26	30.774385735940236	19.560766988112903	21.590970563120944	28.073876712825918
27	31.08841380673556	21.52313429139513	20.382174764222164	27.006277137647146
28	31.63970905269568	21.540253135202118	19.06922791960169	27.75080989250051
29	30.163243012030893	20.132490721016026	19.43116592793149	30.273100339021582
30	29.818264071632544	21.10700943613496	20.091565271754796	28.983161220477697
31	28.000151558830506	21.0567028604675	20.214706821539703	30.72843875916229
32	29.78236014988803	23.23467629521743	18.703067263132837	28.279896291761702
33	30.790705700369564	23.05422085636674	19.63663770386546	26.518435739398242
34	29.40181966462131	22.776142357566084	20.28432345302144	27.53771452479117
35	31.91787885199664	22.682696295505025	19.282597188811945	26.116827663686394
36	33.57779042289707	21.889842750865814	20.538275794616787	23.99409103162033
37	32.183152455629674	21.90262482090836	20.76504341224664	25.14917931121533
38	29.79521069530581	24.811162033965157	22.119646110130773	23.273981160598264
39	30.960752882185606	25.185494085211214	21.941290582787072	21.912462449816108
40	31.01108228297814	25.824551937088476	19.18992718100347	23.97443859892991
41	30.631226551463698	25.9102374566237	18.90075567141589	24.55778032049671
42	31.9256165693974	25.165681876645262	18.539799143464357	24.368902410492986
43	31.70268357278031	24.14526183499854	18.510035180365282	25.64201941185587
44	29.64296711104903	25.244063356156175	18.500311677082912	26.612657855711884
45	26.74876407653995	24.45951815704462	19.054300287802004	29.737417478613427
46	27.470471706317333	23.418441377204136	20.40105114266	28.710035773818532
47	29.281645381096276	21.259093244516205	21.795666284802323	27.66359508958519
48	29.374703416031046	20.99464134538591	21.18847230753113	28.442182931051917
49	28.893823680930314	21.836934110939694	21.32877835137317	27.94046385675682
50	28.659866148901532	22.911175797515575	20.83475134422868	27.594206709354214
51	28.128337461257203	22.55770591058896	22.392794381915017	26.921162246238815
52	29.042323944674635	22.453372263866665	22.702508504070977	25.801795287387723
53	28.531680246474835	22.953972907033037	22.493772735251145	26.020574111240986
54	26.59111093764016	22.737362470061996	22.9029815776133	27.76854501468454
55	26.77311848499602	22.624515051686355	22.261778163978892	28.34058829933873
56	26.952820694719204	23.363524126271333	23.085285851595145	26.59836932741432
57	27.125652541794516	23.01402581110794	23.26361855381377	26.596703093283775
58	28.281653826392557	21.189750514535383	23.29824426855403	27.23035139051803
59	26.832167083567576	22.477498421071974	21.860672241018715	28.829662254341738
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	3.0
7	6.0
8	39.0
9	210.5
10	349.0
11	524.0
12	699.0
13	857.5
14	1195.0
15	1374.0
16	1520.5
17	1667.0
18	1764.5
19	2192.0
20	2522.0
21	3133.0
22	3744.0
23	5212.5
24	6681.0
25	8648.5
26	13899.0
27	17182.0
28	20339.0
29	23496.0
30	26402.0
31	32339.0
32	35370.0
33	38732.5
34	42095.0
35	42433.5
36	45896.0
37	49020.0
38	51295.5
39	53571.0
40	56273.0
41	58975.0
42	69268.0
43	83350.5
44	87140.0
45	87917.5
46	88695.0
47	100373.0
48	122079.5
49	132108.0
50	149179.0
51	166250.0
52	189738.0
53	230357.0
54	247488.0
55	268269.5
56	289051.0
57	327002.0
58	390927.0
59	416901.0
60	401274.0
61	385647.0
62	359063.5
63	332480.0
64	303015.0
65	250060.0
66	226570.0
67	190360.0
68	154150.0
69	140731.5
70	115460.5
71	103608.0
72	91330.5
73	79053.0
74	71624.5
75	48021.5
76	31847.0
77	21324.0
78	10801.0
79	8962.0
80	7123.0
81	4840.5
82	1737.0
83	916.0
84	879.5
85	843.0
86	581.0
87	295.5
88	272.0
89	156.0
90	40.0
91	35.0
92	15.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	4381137.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.299731605850038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.25611035079741	10.806844590009387
2	6.802545794582551	1.809440666089036
3	2.95873125626016	1.180509948062989
4	1.704706094238041	0.9068853408089137
5	1.0885097166818265	0.7238443541114078
6	0.697092481468361	0.5562685744791116
7	0.46257996479260305	0.4306532564589626
8	0.33676341760567163	0.35830904550593806
9	0.2453076889850199	0.29362737819168905
>10	1.6786790760195316	4.573083218624427
>50	0.6113684134820914	6.132279808760624
>100	2.008721246746522	58.7689298931626
>500	0.1405162829767488	12.009822243999203
>1k	0.008368215363379582	1.4495016817357287
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0026477145088135796	0.0	0.0	0.0	0.0
12	0.0063453847711222	0.0	0.0	0.0	0.0
13	0.00821704502735249	0.0	0.0	0.0	0.0
14	0.015749336302425605	0.0	0.0	0.0	0.0
15	0.028189029468834233	0.0	0.0	0.0	0.0
16	0.04110805026183842	0.0	0.0	0.0	0.0
17	0.05373034442885488	0.0	0.0	0.0	0.0
18	0.06920577923036873	0.0	0.0	0.0	0.0
19	0.08577682003552958	0.0	0.0	0.0	0.0
20	0.10369454322017321	0.0	0.0	0.0	0.0
21	0.12227419503202022	0.0	0.0	0.0	0.0
22	0.1413559995955388	0.0	0.0	0.0	0.0
23	0.16007260215784166	0.0	0.0	0.0	0.0
24	0.18091194135221061	0.0	0.0	0.0	0.0
25	0.20282406142515058	0.0	0.0	0.0	2.2825125075979134E-5
26	0.2274295462570561	0.0	0.0	0.0	2.2825125075979134E-5
27	0.2528339104666209	0.0	0.0	0.0	2.2825125075979134E-5
28	0.2768915922967029	0.0	0.0	0.0	2.2825125075979134E-5
29	0.3048751956398533	0.0	0.0	0.0	2.2825125075979134E-5
30	0.33721839787251573	0.0	0.0	0.0	2.2825125075979134E-5
31	0.37109088348526875	0.0	0.0	0.0	2.2825125075979134E-5
32	0.39727130194741683	0.0	0.0	0.0	2.2825125075979134E-5
33	0.41649005726139127	0.0	0.0	0.0	2.2825125075979134E-5
34	0.43513818444846625	0.0	0.0	0.0	2.2825125075979134E-5
35	0.4519146513793109	0.0	0.0	0.0	2.2825125075979134E-5
36	0.4700606258147143	0.0	0.0	0.0	2.2825125075979134E-5
37	0.48818377512504174	0.0	0.0	0.0	2.2825125075979134E-5
38	0.5083611856922073	0.0	0.0	0.0	2.2825125075979134E-5
39	0.5345644292794314	0.0	0.0	0.0	2.2825125075979134E-5
40	0.5651500968812434	0.0	0.0	0.0	2.2825125075979134E-5
41	0.5920380942207468	0.0	0.0	0.0	2.2825125075979134E-5
42	0.6165294534272724	0.0	0.0	0.0	2.2825125075979134E-5
43	0.6392176277527957	0.0	0.0	0.0	2.2825125075979134E-5
44	0.6612666985761915	0.0	0.0	0.0	2.2825125075979134E-5
45	0.6841831241524746	0.0	0.0	0.0	2.2825125075979134E-5
46	0.7071451999789096	0.0	0.0	0.0	2.2825125075979134E-5
47	0.7267291572940997	0.0	0.0	0.0	2.2825125075979134E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	30	7.015042E-7	53.000004	27
AATCACG	15	0.006697516	53.000004	30
CTAACGT	30	7.015042E-7	53.000004	53
ATATACG	30	7.015042E-7	53.000004	14
ATACGTC	30	7.015042E-7	53.000004	46
CGATATT	15	0.006697516	53.000004	50
TAATCGC	15	0.006697516	53.000004	25
CCGATTA	95	0.0	53.0	49
CGATTTA	40	1.6025297E-9	53.0	33
ATCCGAT	35	3.3449396E-8	53.0	40
TTACGTA	25	1.47942155E-5	53.0	18
ACGTATA	20	3.1392204E-4	53.0	35
TAACTAG	20	3.1392204E-4	53.0	40
CGATCTA	3575	0.0	50.62797	2
TACGTCT	1475	0.0	50.48474	1
ACCGGGG	264630	0.0	50.314247	8
TTATCTA	14650	0.0	50.286694	2
CGTAATT	95	0.0	50.21053	45
ACGTCTA	4930	0.0	50.20487	2
TTTCTAC	61775	0.0	50.061516	3
>>END_MODULE