FastQCFastQC Report
Fri 17 Jun 2016
SRR1527978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807840
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA551.8189894E-1246.00000421
CTATAGC551.8189894E-1246.00000438
TCTTACG551.8189894E-1246.00000425
TGTATCG551.8189894E-1246.00000425
TTCAAGC551.8189894E-1246.00000432
CGCGATT551.8189894E-1246.00000419
TAAAACC551.8189894E-1246.00000419
ATCCGTT551.8189894E-1246.00000433
AACATAC551.8189894E-1246.00000438
TACGAGT551.8189894E-1246.00000418
TACGAAA551.8189894E-1246.00000414
ACTACGT1100.046.00000430
ATCGCAT551.8189894E-1246.00000420
CGAAGTT551.8189894E-1246.00000437
TAATCAG253.417938E-546.036
TCACGGC301.8619994E-646.036
TTGATCA253.417938E-546.024
TATTTAC501.6370905E-1146.036
TTAGGTT301.8619994E-646.017
AGGTATA301.8619994E-646.046
Produced by FastQC (version 0.11.5)