FastQCFastQC Report
Fri 17 Jun 2016
SRR1527977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794378
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACC700.046.00000443
AACCGAC351.0199983E-746.00000441
ATAATAC351.0199983E-746.00000440
TCTTACG351.0199983E-746.00000425
CGACCTA351.0199983E-746.00000417
ATAGCAA351.0199983E-746.00000439
CGCGATT700.046.00000419
CGATTAT351.0199983E-746.00000437
CAACACG750.046.00000439
TTATCGA750.046.00000442
ACCCTAA351.0199983E-746.00000426
TAGTTTA351.0199983E-746.00000436
TATGAAG351.0199983E-746.00000428
GACTTAC351.0199983E-746.00000431
TCTACGG700.046.00000415
TAATTGA351.0199983E-746.00000424
CACTAGA351.0199983E-746.00000418
CCTCATA351.0199983E-746.00000426
GATATCC351.0199983E-746.00000419
GTTTTAC351.0199983E-746.00000440
Produced by FastQC (version 0.11.5)