FastQCFastQC Report
Fri 17 Jun 2016
SRR1527976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810886
Sequences flagged as poor quality0
Sequence length59
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT150.006696885553.00000434
TAATAGA307.0127317E-753.00000445
ACAAATT150.006696885553.00000426
AATCATA307.0127317E-753.00000430
CATATGA307.0127317E-753.00000433
CTACTAT150.006696885553.00000440
TACTTAT150.006696885553.00000446
TGCCATA150.006696885553.00000452
CAACGTT307.0127317E-753.00000420
CCGATAC150.006696885553.00000430
GTTTACT150.006696885553.00000443
CTATATA150.006696885553.00000432
GGCAATT150.006696885553.00000427
CACATAG150.006696885553.00000423
ATTACGA150.006696885553.00000444
TATATGT150.006696885553.00000451
AAGGTTA150.006696885553.00000446
TATATCA150.006696885553.00000441
CTAAGTC150.006696885553.00000445
CAATTGA150.006696885553.00000415