##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527976_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1810886
Sequences flagged as poor quality	0
Sequence length	59
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.468110085339443	31.0	31.0	34.0	30.0	34.0
2	31.992243023580723	33.0	31.0	34.0	30.0	34.0
3	32.212530220013846	34.0	31.0	34.0	30.0	34.0
4	35.88822764105526	37.0	35.0	37.0	35.0	37.0
5	35.939693608543	37.0	35.0	37.0	35.0	37.0
6	36.0760130676365	37.0	35.0	37.0	35.0	37.0
7	35.63811084739735	37.0	35.0	37.0	33.0	37.0
8	35.13078901708887	37.0	35.0	37.0	32.0	37.0
9	36.6910782898537	38.0	37.0	39.0	32.0	39.0
10	37.421501960918576	39.0	37.0	39.0	35.0	39.0
11	37.61170112309665	39.0	37.0	39.0	35.0	39.0
12	37.63457059141216	39.0	37.0	39.0	35.0	39.0
13	37.60646722101778	39.0	37.0	39.0	35.0	39.0
14	38.01748425908644	40.0	38.0	40.0	34.0	40.0
15	37.34917217317932	40.0	37.0	40.0	32.0	40.0
16	36.819895896262935	39.0	35.0	40.0	31.0	40.0
17	36.73424831822655	39.0	35.0	40.0	31.0	40.0
18	36.73932870429171	39.0	35.0	40.0	30.0	40.0
19	36.93301842302608	39.0	36.0	40.0	31.0	40.0
20	37.067354874906535	39.0	36.0	40.0	31.0	40.0
21	36.97727245116479	39.0	35.0	40.0	31.0	40.0
22	36.90011022228898	39.0	35.0	40.0	31.0	40.0
23	36.85066426047802	39.0	35.0	40.0	31.0	40.0
24	36.749167534565956	39.0	35.0	40.0	31.0	40.0
25	36.59209193731687	39.0	35.0	40.0	30.0	40.0
26	36.34334905676006	39.0	35.0	40.0	30.0	40.0
27	36.12343018831666	39.0	35.0	40.0	30.0	40.0
28	36.02964902263312	38.0	35.0	40.0	30.0	40.0
29	35.932811342072334	38.0	35.0	40.0	29.0	40.0
30	35.78391847968343	38.0	35.0	40.0	29.0	40.0
31	35.70228440663852	38.0	35.0	40.0	29.0	40.0
32	35.419942503282925	37.0	34.0	40.0	28.0	40.0
33	35.155147259407826	37.0	34.0	40.0	28.0	40.0
34	35.01209297548272	37.0	34.0	40.0	28.0	40.0
35	34.7691014232812	36.0	33.0	39.0	27.0	40.0
36	34.47222464583635	35.0	33.0	39.0	27.0	40.0
37	34.195645667369455	35.0	33.0	39.0	26.0	40.0
38	33.89235711138084	35.0	33.0	39.0	26.0	40.0
39	33.68993796406842	35.0	33.0	38.0	26.0	40.0
40	33.44606286646426	35.0	33.0	38.0	25.0	40.0
41	33.24624189485147	35.0	32.0	37.0	24.0	40.0
42	33.083845145415005	35.0	32.0	37.0	24.0	40.0
43	32.788034144612084	35.0	32.0	37.0	23.0	39.0
44	32.56936549291341	35.0	32.0	36.0	23.0	39.0
45	32.3337730812431	35.0	31.0	36.0	23.0	39.0
46	32.13707709927627	35.0	31.0	35.0	23.0	39.0
47	32.0212663856256	35.0	31.0	35.0	23.0	38.0
48	31.842036991837144	34.0	31.0	35.0	22.0	37.0
49	31.738713535805125	34.0	31.0	35.0	22.0	37.0
50	31.540375263821137	34.0	31.0	35.0	22.0	37.0
51	31.405369526298177	34.0	31.0	35.0	22.0	36.0
52	31.224702714582808	34.0	31.0	35.0	20.0	36.0
53	31.025819405528566	34.0	30.0	35.0	20.0	36.0
54	30.90290774792008	34.0	30.0	35.0	20.0	36.0
55	30.786486835725718	34.0	30.0	35.0	20.0	35.0
56	30.644540849065045	34.0	30.0	35.0	20.0	35.0
57	30.492308185054167	34.0	30.0	35.0	20.0	35.0
58	30.324001069089938	34.0	29.0	35.0	18.0	35.0
59	29.869743319016216	33.0	29.0	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	5.0
13	8.0
14	20.0
15	83.0
16	261.0
17	664.0
18	1560.0
19	3078.0
20	5155.0
21	7332.0
22	10305.0
23	14003.0
24	18123.0
25	23394.0
26	29321.0
27	35747.0
28	42463.0
29	50217.0
30	59234.0
31	71160.0
32	87773.0
33	110755.0
34	158257.0
35	248511.0
36	369976.0
37	320712.0
38	136136.0
39	6628.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.481875722712527	23.207700539956686	32.95613307519082	20.354290662139967
2	26.10313404598633	23.274849990557108	33.03432684332421	17.587689120132353
3	16.87974836626933	25.579688616511476	35.86393621685738	21.676626800361813
4	16.681668531315612	26.892581863242633	34.13312599467885	22.292623610762906
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	0.0	100.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	0.0	100.0
11	98.34302104052934	1.0470565237127019	0.0849308018284972	0.5249916339294688
12	98.63431491546127	1.1399944557525985	0.0734447115942141	0.152245917191916
13	96.60177393828214	1.972018117098481	0.6009213169685999	0.8252866276507743
14	61.8347593387988	22.547968232125047	6.422712418120192	9.194560010955962
15	34.19624426937974	25.74894278270416	17.86699991054103	22.187813037375076
16	27.498914895802386	17.416170868845416	28.59235755315354	26.49255668219866
17	23.299313153892626	16.783994133258524	30.46442459657869	29.452268116270158
18	23.688735790104953	17.304071045885827	30.96572616939995	28.04146699460927
19	24.261604540539825	16.41157974604696	27.345288438918853	31.981527274494365
20	28.682258297871872	16.893553763185533	23.949768234996572	30.474419703946026
21	30.04689417224497	17.981639926533198	20.7714897569477	31.199976144274128
22	28.781822820431547	19.2194318140402	21.669227107614724	30.32951825791353
23	29.994875436664703	18.785776686108346	19.7125053702994	31.506842506927548
24	29.469331586858587	19.8173711652749	21.090946641588705	29.62235060627781
25	33.74072139273262	16.09620925889316	21.17322680720929	28.989842541164933
26	33.91947367200365	16.387668798588095	20.057529850029212	29.635327679379042
27	31.462554793620363	19.684508025353338	19.26405085687338	29.58888632415293
28	30.30869971936389	19.82322465356737	19.88661903620659	29.981456590862155
29	32.88053472167768	18.225111906547404	18.77903965241324	30.11531371936168
30	30.113491406968745	19.20512942283501	19.132126483942113	31.549252686254132
31	32.522698833609624	17.654341576443798	20.15792269640386	29.66503689354272
32	32.79969031733638	20.155272060195948	17.869595325161274	29.175442297306404
33	31.06661601006358	21.000051908292406	19.965033690690635	27.96829839095338
34	29.627872765044295	21.94605292657848	20.108941148145163	28.31713316023206
35	32.0981000460548	22.387162968845082	18.49619468039402	27.018542304706095
36	34.295587905588754	21.853556767239905	17.901568624419205	25.949286702752133
37	34.27570813402942	20.251578509083398	19.619346551908844	25.853366804978332
38	31.52727449436353	24.22178977583349	21.424153701558243	22.82678202824474
39	33.04073254749333	22.870848855201267	21.262741000813968	22.825677596491442
40	32.78798334075143	22.627763426300717	18.321749684960842	26.26250354798701
41	32.55296026364995	23.55095792888122	17.545886378270083	26.35019542919875
42	32.2379763276098	23.25971927553695	17.302690506194203	27.199613890659048
43	32.919134611455384	22.535322488549802	18.648551040761262	25.896991859233548
44	31.86048155433307	23.287550955720018	18.748667779197586	26.103299710749322
45	29.371368490341194	22.585518911737125	19.864585622728324	28.178526975193357
46	29.38760363711465	19.908762892860178	22.1221545696416	28.581478900383566
47	29.673706682806095	18.996888815750964	24.609997537117188	26.71940696432575
48	28.908777250472973	20.156045162423254	22.364743004253164	28.570434582850606
49	30.713363513771714	19.207614394279926	21.98316183348924	28.095860258459123
50	29.437082179662333	21.61593827551817	20.200829870019426	28.74614967480007
51	30.45757711970825	21.52603753079984	21.755869778660834	26.26051557083107
52	30.457135347006936	20.987571829480157	21.805403542796178	26.749889280716733
53	27.39581619163216	22.223983177295533	22.262251737547256	28.117948893525046
54	27.826378910654785	21.32381607677126	22.16826459534173	28.681540417232227
55	29.015631022604403	22.314877910591832	20.477434802632523	28.19205626417124
56	30.109460231069214	22.193390417729223	20.314972891722615	27.382176459478952
57	28.942462418948516	21.149702410864073	21.300733453127364	28.607101717060047
58	29.262858070579817	20.556567337756213	21.84505264273952	28.33552194892445
59	27.84211706313926	21.59434663474123	22.339175409164355	28.224360892955165
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.5
9	8.5
10	14.0
11	63.5
12	113.0
13	145.5
14	208.0
15	238.0
16	301.5
17	365.0
18	390.0
19	385.5
20	356.0
21	479.5
22	603.0
23	759.0
24	915.0
25	1471.0
26	2716.0
27	3405.0
28	4548.0
29	5691.0
30	6535.5
31	8099.0
32	8818.0
33	9285.0
34	9752.0
35	10573.5
36	10874.5
37	10354.0
38	12018.5
39	13683.0
40	13038.0
41	12393.0
42	13207.5
43	16478.0
44	18934.0
45	21703.5
46	24473.0
47	25806.5
48	32764.5
49	38389.0
50	42293.0
51	46197.0
52	51285.5
53	66931.0
54	77488.0
55	88455.5
56	99423.0
57	112151.0
58	143541.0
59	162203.0
60	171548.5
61	180894.0
62	178070.5
63	175247.0
64	165080.0
65	139067.5
66	123222.0
67	116742.5
68	110263.0
69	100969.0
70	77571.0
71	63467.0
72	56225.0
73	48983.0
74	42599.0
75	31802.0
76	27389.0
77	18905.0
78	10421.0
79	8225.0
80	6029.0
81	3913.0
82	1592.0
83	1387.0
84	1002.0
85	617.0
86	487.5
87	266.0
88	174.0
89	145.0
90	116.0
91	92.0
92	48.0
93	28.0
94	15.5
95	3.0
96	1.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1810886.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.50271397040595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.6280848367404	11.157033954729426
2	6.647952207650899	1.7953079429767773
3	2.818233705288314	1.1416141087279632
4	1.6092535766981644	0.8691716300803219
5	0.9407289332268426	0.6351196854523586
6	0.6137006407070933	0.49719745289536504
7	0.44419569204769166	0.4198493163624554
8	0.28969596925621366	0.3129345448996935
9	0.2121458202870303	0.2578089898217623
>10	1.3923170881666516	3.81869816621542
>50	0.3818108654871806	3.804956137628928
>100	1.8518235766132634	59.67223074542925
>500	0.15983731091739206	14.009666013945484
>1k	0.010219776912876729	1.6084113108347946
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.004307283837856166	0.0	0.0	0.0	0.0
12	0.007178806396426943	0.0	0.0	0.0	0.0
13	0.008504124500382685	0.0	0.0	0.0	0.0
14	0.01761568646507842	0.0	0.0	0.0	0.0
15	0.02661680525444451	0.0	0.0	0.0	0.0
16	0.03821333866405726	0.0	0.0	0.0	0.0
17	0.04909199143402732	0.0	0.0	0.0	0.0
18	0.060633303255975254	0.0	0.0	0.0	0.0
19	0.07256116619157695	0.0	0.0	0.0	0.0
20	0.08741577327341422	0.0	0.0	0.0	0.0
21	0.1029882609948942	0.0	0.0	0.0	0.0
22	0.11673843632343504	0.0	0.0	0.0	0.0
23	0.13109604911628894	0.0	0.0	0.0	0.0
24	0.14490144603249458	0.0	0.0	0.0	0.0
25	0.1607500416922987	0.0	0.0	0.0	0.0
26	0.17864183609570122	0.0	0.0	0.0	0.0
27	0.19603663621012035	0.0	0.0	0.0	0.0
28	0.21541941348047308	0.0	0.0	0.0	0.0
29	0.23701105425741875	0.0	0.0	0.0	0.0
30	0.2642352969761763	0.0	0.0	0.0	0.0
31	0.29195653398391724	0.0	0.0	0.0	0.0
32	0.3147078281018242	0.0	0.0	0.0	0.0
33	0.330335537410969	0.0	0.0	0.0	0.0
34	0.3478407807007178	0.0	0.0	0.0	0.0
35	0.36479380811381834	0.0	0.0	0.0	0.0
36	0.3812498412379355	0.0	0.0	0.0	0.0
37	0.39709843689773955	0.0	0.0	0.0	0.0
38	0.4170886516324053	0.0	0.0	0.0	0.0
39	0.4387907355846806	0.0	0.0	0.0	0.0
40	0.46419266591049907	0.0	0.0	0.0	0.0
41	0.4872200679667301	0.0	0.0	0.0	0.0
42	0.5079833849287034	0.0	0.0	0.0	0.0
43	0.5312868949232585	0.0	0.0	0.0	0.0
44	0.5536516379275117	0.0	0.0	0.0	0.0
45	0.576899926334402	0.0	0.0	0.0	0.0
46	0.5974423569457161	0.0	0.0	0.0	0.0
47	0.616162475164091	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAT	15	0.0066968855	53.000004	34
TAATAGA	30	7.0127317E-7	53.000004	45
ACAAATT	15	0.0066968855	53.000004	26
AATCATA	30	7.0127317E-7	53.000004	30
CATATGA	30	7.0127317E-7	53.000004	33
CTACTAT	15	0.0066968855	53.000004	40
TACTTAT	15	0.0066968855	53.000004	46
TGCCATA	15	0.0066968855	53.000004	52
CAACGTT	30	7.0127317E-7	53.000004	20
CCGATAC	15	0.0066968855	53.000004	30
GTTTACT	15	0.0066968855	53.000004	43
CTATATA	15	0.0066968855	53.000004	32
GGCAATT	15	0.0066968855	53.000004	27
CACATAG	15	0.0066968855	53.000004	23
ATTACGA	15	0.0066968855	53.000004	44
TATATGT	15	0.0066968855	53.000004	51
AAGGTTA	15	0.0066968855	53.000004	46
TATATCA	15	0.0066968855	53.000004	41
CTAAGTC	15	0.0066968855	53.000004	45
CAATTGA	15	0.0066968855	53.000004	15
>>END_MODULE