FastQCFastQC Report
Fri 17 Jun 2016
SRR1527975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126846
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTGTGTGGGGGTCTGTGTGGGAGGCAGGCAGATCACTTGAGCCCAGGA13431.058764170726708No Hit
AAGACTGTGTGGGGACTTGGAGCAGCTCTGCCGCGGGGACTGCACTGTCCGC8600.6779874808823297No Hit
GAGGCTGTGTGGGTCCCACCAGAAATTGAGGAGTCCCATCTGAGTCTTTTGC6080.4793213818330889No Hit
AATGCTGTGTGGGGCGGATTACCTGAGGTCAGGAGTTCCAGACCAACCTGAC6050.4769563092253599No Hit
GCTCCTGTGTATAAGAATGAACTAGAGTTGTCATTTTTCTCTTCACTGGATG5800.4572473708276178No Hit
ATGGCTGTGTGGGGCAGAGTCTGCAAAAAGAGAGATTCAAACTGCTGAATCA5100.40206234331393975No Hit
CGTGCTGTGTGGTCGGGGACAGTGGCTCATGCCTGTAATCCCAGCAGTTTAG4890.38550683505983635No Hit
CTGTCTGTGTGGGGGCACTGTGCTAAACAATCTACAAAGCTTCTTCCAGCTC4850.3823534049161976No Hit
TGGCCTGTGTGGGCCCCCTTCTGGAGGGCTTCCACTGCCCTTCCAGGCTGGG4790.3776232597007395No Hit
GCTTCTGTGTGGGGGTGGGGACCACGTTCCAGGCGCCGGTGGACAGGTACAT3220.2538511265629188No Hit
AGTGCTGTGTGGGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC3070.24202576352427355No Hit
AGTTCTGTGTGGGGGCGGGCGGATAATGAGGTCAGGAGATCGAGACCATTGT2950.23256547309335732No Hit
GTACCTGTGTGGGTTGGGAGGGCCACCCAACCCTTCCCTGATGAACTCCCCC2910.22941204294971854No Hit
GTCGCTGTGTAGGGTTCCGATGTTACCGGTTGGCGGTATCACACCGGAAACC2800.220740110054712No Hit
GTGCCTGTGTGGTGCAGATATGGCTGCAGTGACCAAAAACTTAGATCACAAC2790.2199517525188023No Hit
GACTCTGTGTTTGTCTGTTTAGTTCTGTGTGTAAATGAAATGTGGAAATGAC2700.21285653469561516No Hit
TGTCCTGTGTGGGAGCCTTGGCTTTAACCCAACTAAATCAGAAGTGTTTGGT2700.21285653469561516No Hit
TTTTCTGTGTGGGGCGGACCCAGGTCTACTTGCTTCCTTCCCATTACACAAA2340.18447566340286647No Hit
GCGTCTGTGTGGGGAGGCTGGGAAATACTAAATTTAATCAGTGTAGCCAGGG2300.18132223325922772No Hit
GAAGCTGTGTGGGTATGTGGTGTGTGTGTGTGGGTGTGTGTTGTGTGTGTGT2180.1718619428283115No Hit
TTGCCTGTGTGGGGAAGGGATCCAGTTTCAGCTTTCTACATATGTCTAGCCA2020.15924822225375654No Hit
CGGACTGTGTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC1950.15372971950238873No Hit
GGCCCTGTGTGAGGAACGATTGGTGTTGCTGCTGGTGGTTGGGAGTAGTTGT1930.15215300443056937No Hit
CATCCTGTGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG1750.137962568784195No Hit
GCGGCTGTGTGGGTGGGAAGCTATACTATATGGGAGGCTCTCTCATTGGTTT1750.137962568784195No Hit
AAGGCTGTGTGGGGTGGTATCAACGCAGATTATTCCTGTGTGGGATAAAACA1550.12219541806600129No Hit
GGTTCTGTGTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT1510.11904198792236254No Hit
AAATCTGTGTGGAGAATCTGCCGCGAATGCCGCCTGAGAAAAAAGAGAAGCA1480.1166769153146335No Hit
TGTTCTGTGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG1460.11510020024281412No Hit
GTTGCTGTGTGGGAGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGA1420.11194677009917539No Hit
ATCACTGTGTGGGGGGAATTCACTGTCCTTTCATCCATAAGCGGAGAAAGAG1360.10721662488371728No Hit
GTTTCTGTGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG1330.10485155227598822No Hit
GGGCCTGTGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG1280.10090976459643977No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGT351.0099757E-746.00000417
TAGCCTG351.0099757E-746.0000041
GAAGCAA351.0099757E-746.00000413
TTATTCC351.0099757E-746.00000430
GAACGCA351.0099757E-746.00000413
GTCTGCA700.046.00000419
GCAGTCG351.0099757E-746.00000416
TATCACA351.0099757E-746.00000437
GGGATAC750.046.00000411
TGGGCCC700.046.00000410
GGAACGC351.0099757E-746.00000412
CCGCGAA206.290128E-446.021
CTTTTGC950.046.046
CAGCTCT600.046.023
CGTCATG600.046.037
CGTTCCA253.3998673E-546.025
CGCGAAT253.3998673E-546.022
CGTTTCT600.046.019
ACCGGTT453.0559022E-1046.025
GTATGTG405.5442797E-946.013
Produced by FastQC (version 0.11.5)