##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527975_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	126846
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.888210901408005	31.0	31.0	33.0	30.0	33.0
2	31.726132475600334	31.0	31.0	34.0	30.0	34.0
3	31.984256500007884	33.0	31.0	34.0	30.0	34.0
4	35.75370922220645	37.0	35.0	37.0	35.0	37.0
5	35.95055421534774	37.0	35.0	37.0	35.0	37.0
6	35.961133973479654	37.0	35.0	37.0	35.0	37.0
7	35.86601075319679	37.0	35.0	37.0	35.0	37.0
8	34.368911908928936	35.0	35.0	36.0	32.0	37.0
9	37.04067924885294	39.0	37.0	39.0	33.0	39.0
10	35.77613799410309	37.0	35.0	39.0	32.0	39.0
11	37.767568547687745	39.0	37.0	39.0	35.0	39.0
12	38.19559150465919	39.0	39.0	39.0	37.0	39.0
13	38.13156110559261	39.0	39.0	39.0	37.0	39.0
14	38.58257256831118	40.0	39.0	40.0	36.0	40.0
15	37.717499960582124	40.0	37.0	40.0	33.0	40.0
16	36.59915961086672	38.0	35.0	40.0	31.0	40.0
17	36.27992211027545	38.0	35.0	40.0	31.0	40.0
18	36.34643583558015	38.0	35.0	40.0	31.0	40.0
19	36.62374848241174	39.0	35.0	40.0	31.0	40.0
20	36.71727133689671	39.0	35.0	40.0	32.0	40.0
21	36.713637008656164	39.0	35.0	40.0	31.0	40.0
22	36.92922914400139	39.0	35.0	40.0	32.0	40.0
23	36.84033394825221	39.0	35.0	40.0	32.0	40.0
24	36.975884142976525	39.0	35.0	40.0	32.0	40.0
25	36.681456254040334	39.0	35.0	40.0	31.0	40.0
26	36.62124938902291	39.0	35.0	40.0	31.0	40.0
27	36.319174431988394	38.0	35.0	40.0	31.0	40.0
28	36.21139018967882	38.0	35.0	40.0	31.0	40.0
29	36.06258770477587	38.0	35.0	40.0	31.0	40.0
30	35.93559118931618	38.0	35.0	40.0	30.0	40.0
31	35.702875928291	38.0	35.0	40.0	30.0	40.0
32	35.39326427321319	37.0	35.0	40.0	29.0	40.0
33	35.02371379468016	36.0	34.0	40.0	26.0	40.0
34	34.72576983113382	36.0	34.0	40.0	25.0	40.0
35	34.41086829699005	35.0	33.0	40.0	24.0	40.0
36	34.112143859483155	35.0	33.0	40.0	22.0	40.0
37	33.898680289484886	35.0	33.0	40.0	22.0	40.0
38	33.518810210806805	35.0	33.0	40.0	21.0	40.0
39	33.025889661479276	35.0	33.0	40.0	18.0	40.0
40	32.45228071835139	35.0	33.0	39.0	15.0	40.0
41	31.76684325875471	35.0	31.0	39.0	12.0	40.0
42	31.03798306608013	35.0	30.0	38.0	10.0	40.0
43	30.26789965785283	35.0	26.0	38.0	9.0	40.0
44	29.529137694527222	35.0	23.0	37.0	8.0	40.0
45	28.88257414502625	35.0	22.0	36.0	8.0	40.0
46	28.23910095706605	35.0	20.0	36.0	8.0	40.0
47	27.71350298787506	34.0	18.0	35.0	8.0	40.0
48	27.176907431058133	33.0	15.0	35.0	8.0	40.0
49	26.708386547467008	33.0	12.0	35.0	8.0	40.0
50	26.213991769547324	33.0	10.0	35.0	8.0	40.0
51	25.748277438784037	33.0	10.0	35.0	8.0	40.0
52	24.9254686785551	32.0	10.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	2.0
16	13.0
17	59.0
18	105.0
19	208.0
20	393.0
21	590.0
22	810.0
23	1218.0
24	1681.0
25	2327.0
26	3046.0
27	3757.0
28	4866.0
29	6023.0
30	7062.0
31	7298.0
32	6945.0
33	6707.0
34	8005.0
35	10936.0
36	13440.0
37	19542.0
38	17481.0
39	4330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.54443971429923	24.70554846033773	31.050249909338884	3.6997619160241553
2	33.24030714409599	21.089352443120006	28.786875423742174	16.88346498904183
3	22.785897860397647	19.76491178279173	36.78791605568958	20.661274301121043
4	30.35413020513063	25.37565236586097	23.900635416173944	20.36958201283446
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	100.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	97.77446667612696	1.3977579111678728	0.5234694038440313	0.30430600886113873
12	97.59235608533182	0.905822808760229	1.2456049067373034	0.25621619917064786
13	95.68453084843038	2.2964855021049146	1.4143134194219762	0.604670230042729
14	59.46423221859578	23.470980559103165	9.065323305425476	7.99946391687558
15	40.68555571322706	34.79337148983807	14.12894375857339	10.392129038361478
16	45.44565851504975	25.48681077842423	19.328950065433677	9.738580641092348
17	30.8074357882787	27.872380682086938	22.153635116598082	19.16654841303628
18	25.00906611166296	37.08670356179935	20.833924601485265	17.070305725052425
19	28.553521593112908	39.196348327893666	22.766188921999905	9.48394115699352
20	20.815004020623434	38.76590511328698	24.434353467984803	15.984737398104789
21	22.619554420320704	39.660690916544475	19.703419894990777	18.01633476814405
22	24.729199186415023	38.570392444381376	16.76836478879902	19.932043580404585
23	17.652113586553774	40.2251549122558	27.62956656102676	14.493164940163664
24	25.845513457263138	42.5445027829021	17.420336470996325	14.189647288838433
25	24.545511880548066	39.372152058401525	25.677593302114378	10.404742758936033
26	27.475048483988456	44.088106838213264	17.35017265030036	11.086672027497912
27	24.229380508648283	44.091260268356905	22.665279157403464	9.014080065591347
28	32.68057329360011	43.55596550147423	12.543556753859011	11.219904451066649
29	31.667533859956166	44.17876795484288	11.911294010059443	12.24240417514151
30	34.885609321539505	47.10672784321145	9.996373555334817	8.011289279914227
31	30.193305267805055	49.08787033095249	11.2892799142267	9.42954448701575
32	26.96971130347035	54.499156457436584	9.204862589281491	9.326269649811584
33	22.6763161629062	56.7554357252101	10.100436750074895	10.467811361808806
34	17.85945161849802	61.129243334437035	12.51596424010217	8.495340806962774
35	21.17449505699825	58.933667596928565	11.356290304779023	8.53554704129417
36	15.666240953597274	61.394919824038595	14.151017769578859	8.787821452785268
37	15.912996862337007	62.3716948110307	12.798984595493748	8.916323731138547
38	16.215726156126326	62.85259290793561	11.083518597354272	9.848162338583794
39	14.92518486984217	63.505352947668825	11.974362612932216	9.595099569556785
40	15.141194834681423	60.17927250366586	11.54391939832553	13.135613263327183
41	16.869274553395456	58.04597701149425	14.513662236097316	10.571086199012976
42	19.161029910284913	56.58199706730996	14.40092710846223	9.856045913942891
43	21.881651766709236	56.324204153067505	11.827728111253014	9.966415968970248
44	21.437018116456173	54.8586474938114	13.101713889283067	10.602620500449364
45	22.97983381423143	53.658767324156855	12.789524305062832	10.571874556548886
46	22.385412232155527	51.105277265345386	14.050896362518328	12.458414139980764
47	26.26255459375936	49.08314018573704	11.940463238888102	12.713841981615504
48	24.056730208284062	50.97440991438437	12.229790454566954	12.739069422764612
49	23.98735474512401	50.85615628399792	13.249925106034087	11.906563864843985
50	24.68899295208363	48.94911940463239	13.37212052410009	12.989767119183892
51	24.83956924144238	49.82498462702805	12.798984595493748	12.536461536035823
52	23.15957933241884	48.633776390268515	12.9905554767198	15.216088800592845
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	3.5
10	6.0
11	13.0
12	20.0
13	51.5
14	146.0
15	209.0
16	233.5
17	258.0
18	380.5
19	503.0
20	627.0
21	751.0
22	1035.5
23	1320.0
24	1802.5
25	2285.0
26	3064.5
27	3844.0
28	4886.0
29	5928.0
30	6415.0
31	6902.0
32	7220.5
33	7539.0
34	7675.0
35	7811.0
36	8214.0
37	8617.0
38	8405.0
39	7748.0
40	7303.0
41	6717.5
42	6132.0
43	6239.5
44	6347.0
45	6555.0
46	6763.0
47	6230.0
48	5697.0
49	6354.5
50	7012.0
51	6864.0
52	6716.0
53	6379.0
54	6042.0
55	6133.5
56	6225.0
57	5477.0
58	4729.0
59	4215.5
60	3702.0
61	2725.0
62	1748.0
63	1510.0
64	1562.0
65	1852.0
66	1047.5
67	243.0
68	431.5
69	620.0
70	355.0
71	90.0
72	64.0
73	38.0
74	28.5
75	19.0
76	16.0
77	13.0
78	8.5
79	4.0
80	6.5
81	9.0
82	4.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	126846.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.43421156362834
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.8117991023019	49.85967235860807
2	4.933221692998229	5.666713968118821
3	2.1248267058322923	3.6611323967645806
4	1.2655621594169082	2.9074625924349213
5	0.9731925933043251	2.794727464799836
6	0.6986671791141065	2.4076439146681805
7	0.5215982869614154	2.0970310455197643
8	0.4488490522010075	2.062343313939738
9	0.3651188008729908	1.8873279409677879
>10	1.7761794298107147	16.219667943805874
>50	0.024707287277119676	1.0114627185721268
>100	0.048041947483288265	5.872475284991249
>500	0.0068631353547554666	2.493574886082336
>1k	0.0013726270709510933	1.058764170726708
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATCCTGTGTGGGGGTCTGTGTGGGAGGCAGGCAGATCACTTGAGCCCAGGA	1343	1.058764170726708	No Hit
AAGACTGTGTGGGGACTTGGAGCAGCTCTGCCGCGGGGACTGCACTGTCCGC	860	0.6779874808823297	No Hit
GAGGCTGTGTGGGTCCCACCAGAAATTGAGGAGTCCCATCTGAGTCTTTTGC	608	0.4793213818330889	No Hit
AATGCTGTGTGGGGCGGATTACCTGAGGTCAGGAGTTCCAGACCAACCTGAC	605	0.4769563092253599	No Hit
GCTCCTGTGTATAAGAATGAACTAGAGTTGTCATTTTTCTCTTCACTGGATG	580	0.4572473708276178	No Hit
ATGGCTGTGTGGGGCAGAGTCTGCAAAAAGAGAGATTCAAACTGCTGAATCA	510	0.40206234331393975	No Hit
CGTGCTGTGTGGTCGGGGACAGTGGCTCATGCCTGTAATCCCAGCAGTTTAG	489	0.38550683505983635	No Hit
CTGTCTGTGTGGGGGCACTGTGCTAAACAATCTACAAAGCTTCTTCCAGCTC	485	0.3823534049161976	No Hit
TGGCCTGTGTGGGCCCCCTTCTGGAGGGCTTCCACTGCCCTTCCAGGCTGGG	479	0.3776232597007395	No Hit
GCTTCTGTGTGGGGGTGGGGACCACGTTCCAGGCGCCGGTGGACAGGTACAT	322	0.2538511265629188	No Hit
AGTGCTGTGTGGGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC	307	0.24202576352427355	No Hit
AGTTCTGTGTGGGGGCGGGCGGATAATGAGGTCAGGAGATCGAGACCATTGT	295	0.23256547309335732	No Hit
GTACCTGTGTGGGTTGGGAGGGCCACCCAACCCTTCCCTGATGAACTCCCCC	291	0.22941204294971854	No Hit
GTCGCTGTGTAGGGTTCCGATGTTACCGGTTGGCGGTATCACACCGGAAACC	280	0.220740110054712	No Hit
GTGCCTGTGTGGTGCAGATATGGCTGCAGTGACCAAAAACTTAGATCACAAC	279	0.2199517525188023	No Hit
GACTCTGTGTTTGTCTGTTTAGTTCTGTGTGTAAATGAAATGTGGAAATGAC	270	0.21285653469561516	No Hit
TGTCCTGTGTGGGAGCCTTGGCTTTAACCCAACTAAATCAGAAGTGTTTGGT	270	0.21285653469561516	No Hit
TTTTCTGTGTGGGGCGGACCCAGGTCTACTTGCTTCCTTCCCATTACACAAA	234	0.18447566340286647	No Hit
GCGTCTGTGTGGGGAGGCTGGGAAATACTAAATTTAATCAGTGTAGCCAGGG	230	0.18132223325922772	No Hit
GAAGCTGTGTGGGTATGTGGTGTGTGTGTGTGGGTGTGTGTTGTGTGTGTGT	218	0.1718619428283115	No Hit
TTGCCTGTGTGGGGAAGGGATCCAGTTTCAGCTTTCTACATATGTCTAGCCA	202	0.15924822225375654	No Hit
CGGACTGTGTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC	195	0.15372971950238873	No Hit
GGCCCTGTGTGAGGAACGATTGGTGTTGCTGCTGGTGGTTGGGAGTAGTTGT	193	0.15215300443056937	No Hit
CATCCTGTGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG	175	0.137962568784195	No Hit
GCGGCTGTGTGGGTGGGAAGCTATACTATATGGGAGGCTCTCTCATTGGTTT	175	0.137962568784195	No Hit
AAGGCTGTGTGGGGTGGTATCAACGCAGATTATTCCTGTGTGGGATAAAACA	155	0.12219541806600129	No Hit
GGTTCTGTGTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT	151	0.11904198792236254	No Hit
AAATCTGTGTGGAGAATCTGCCGCGAATGCCGCCTGAGAAAAAAGAGAAGCA	148	0.1166769153146335	No Hit
TGTTCTGTGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG	146	0.11510020024281412	No Hit
GTTGCTGTGTGGGAGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGA	142	0.11194677009917539	No Hit
ATCACTGTGTGGGGGGAATTCACTGTCCTTTCATCCATAAGCGGAGAAAGAG	136	0.10721662488371728	No Hit
GTTTCTGTGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG	133	0.10485155227598822	No Hit
GGGCCTGTGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG	128	0.10090976459643977	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.010248647966825914	0.0	0.0	0.0	0.0
12	0.029957586364568058	0.0	0.0	0.0	0.0
13	0.06779874808823297	0.0	0.0	0.0	0.0
14	0.1876290935465052	0.0	0.0	0.0	0.0
15	0.371316399413462	0.0	0.0	0.0	0.0
16	0.4793213818330889	0.0	0.0	0.0	0.0
17	0.5983633697554515	0.0	0.0	0.0	0.0
18	0.7110984973905365	0.0	0.0	0.0	0.0
19	0.8640398593570155	0.0	0.0	0.0	0.0
20	1.058764170726708	0.0	0.0	0.0	0.0
21	1.2109171751572774	0.0	0.0	0.0	0.0
22	1.3961811960960535	0.0	0.0	0.0	0.0
23	1.568043138924365	0.0	0.0	0.0	0.0
24	1.761190735222238	0.0	0.0	0.0	0.0
25	1.98193084527695	0.0	0.0	0.0	0.0
26	2.2665279157403466	0.0	0.0	0.0	0.0
27	2.562161991706479	0.0	0.0	0.0	0.0
28	2.8861769389653595	0.0	0.0	0.0	0.0
29	3.233842612301531	0.0	0.0	0.0	0.0
30	3.633539883007742	0.0	0.0	0.0	0.0
31	4.0663481702221596	0.0	0.0	0.0	0.0
32	4.44081799977926	0.0	0.0	0.0	0.0
33	4.767198019645869	0.0	0.0	0.0	0.0
34	5.128265771092506	0.0	0.0	0.0	0.0
35	5.4451855005282	0.0	0.0	0.0	0.0
36	5.755798369676616	0.0	0.0	0.0	0.0
37	6.039607082604102	0.0	0.0	0.0	0.0
38	6.308437002349305	0.0	0.0	0.0	0.0
39	6.611954653674535	0.0	0.0	0.0	0.0
40	6.935181243397506	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTCGT	35	1.0099757E-7	46.000004	17
TAGCCTG	35	1.0099757E-7	46.000004	1
GAAGCAA	35	1.0099757E-7	46.000004	13
TTATTCC	35	1.0099757E-7	46.000004	30
GAACGCA	35	1.0099757E-7	46.000004	13
GTCTGCA	70	0.0	46.000004	19
GCAGTCG	35	1.0099757E-7	46.000004	16
TATCACA	35	1.0099757E-7	46.000004	37
GGGATAC	75	0.0	46.000004	11
TGGGCCC	70	0.0	46.000004	10
GGAACGC	35	1.0099757E-7	46.000004	12
CCGCGAA	20	6.290128E-4	46.0	21
CTTTTGC	95	0.0	46.0	46
CAGCTCT	60	0.0	46.0	23
CGTCATG	60	0.0	46.0	37
CGTTCCA	25	3.3998673E-5	46.0	25
CGCGAAT	25	3.3998673E-5	46.0	22
CGTTTCT	60	0.0	46.0	19
ACCGGTT	45	3.0559022E-10	46.0	25
GTATGTG	40	5.5442797E-9	46.0	13
>>END_MODULE