FastQCFastQC Report
Fri 17 Jun 2016
SRR1527974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125776
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTGTGTGGGGGTCTGTGTGGGAGGCAGGCAGATCACTTGAGCCCAGGA13211.050279862612899No Hit
AAGACTGTGTGGGGACTTGGAGCAGCTCTGCCGCGGGGACTGCACTGTCCGC8260.6567230632235085No Hit
GCTCCTGTGTATAAGAATGAACTAGAGTTGTCATTTTTCTCTTCACTGGATG5880.4674977738201247No Hit
GAGGCTGTGTGGGTCCCACCAGAAATTGAGGAGTCCCATCTGAGTCTTTTGC5580.44364584658440404No Hit
AATGCTGTGTGGGGCGGATTACCTGAGGTCAGGAGTTCCAGACCAACCTGAC5410.4301297544841623No Hit
TGGCCTGTGTGGGCCCCCTTCTGGAGGGCTTCCACTGCCCTTCCAGGCTGGG4900.3895814781834372No Hit
CGTGCTGTGTGGTCGGGGACAGTGGCTCATGCCTGTAATCCCAGCAGTTTAG4780.38004070728914896No Hit
ATGGCTGTGTGGGGCAGAGTCTGCAAAAAGAGAGATTCAAACTGCTGAATCA4730.3760653860831955No Hit
CTGTCTGTGTGGGGGCACTGTGCTAAACAATCTACAAAGCTTCTTCCAGCTC4130.32836153161175424No Hit
AGTGCTGTGTGGGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC3040.24169952932196923No Hit
AGTTCTGTGTGGGGGCGGGCGGATAATGAGGTCAGGAGATCGAGACCATTGT2770.22023279480982064No Hit
GTCGCTGTGTAGGGTTCCGATGTTACCGGTTGGCGGTATCACACCGGAAACC2760.21943773056862995No Hit
GTGCCTGTGTGGTGCAGATATGGCTGCAGTGACCAAAAACTTAGATCACAAC2740.21784760208624857No Hit
GCTTCTGTGTGGGGGTGGGGACCACGTTCCAGGCGCCGGTGGACAGGTACAT2670.21228215239791373No Hit
GTACCTGTGTGGGTTGGGAGGGCCACCCAACCCTTCCCTGATGAACTCCCCC2570.20433150998600685No Hit
GAAGCTGTGTGGGTATGTGGTGTGTGTGTGTGGGTGTGTGTTGTGTGTGTGT2550.2027413815036255No Hit
TGTCCTGTGTGGGAGCCTTGGCTTTAACCCAACTAAATCAGAAGTGTTTGGT2500.19876606029767208No Hit
GACTCTGTGTTTGTCTGTTTAGTTCTGTGTGTAAATGAAATGTGGAAATGAC2490.19797099605648136No Hit
GCGTCTGTGTGGGGAGGCTGGGAAATACTAAATTTAATCAGTGTAGCCAGGG2450.1947907390917186No Hit
TTTTCTGTGTGGGGCGGACCCAGGTCTACTTGCTTCCTTCCCATTACACAAA2280.1812746469914769No Hit
GCGGCTGTGTGGGTGGGAAGCTATACTATATGGGAGGCTCTCTCATTGGTTT2210.1757091973031421No Hit
TTGCCTGTGTGGGGAAGGGATCCAGTTTCAGCTTTCTACATATGTCTAGCCA2050.16298816944409109No Hit
GGTTCTGTGTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT1830.14549675613789592No Hit
CATCCTGTGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG1790.1423164991731332No Hit
CGGACTGTGTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC1680.13357079252003562No Hit
GGCCCTGTGTGAGGAACGATTGGTGTTGCTGCTGGTGGTTGGGAGTAGTTGT1640.13039053555527286No Hit
ATCACTGTGTGGGGGGAATTCACTGTCCTTTCATCCATAAGCGGAGAAAGAG1470.11687444345503117No Hit
GTTGCTGTGTGGGAGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGA1430.11369418649026843No Hit
GATGCTGTGTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT1410.11210405800788703No Hit
GGGCCTGTGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG1350.1073336725607429No Hit
AAGGCTGTGTGGGGTGGTATCAACGCAGATTATTCCTGTGTGGGATAAAACA1300.10335835135478948No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACTA351.0098847E-746.00000429
TCCCATT351.0098847E-746.00000439
GTTTGGT351.0098847E-746.00000446
CCAACCT700.046.00000443
CTGAGGT700.046.00000423
TAAATCA351.0098847E-746.00000434
ACTGATG351.0098847E-746.00000441
CTTTAAC351.0098847E-746.00000422
AAATTGA351.0098847E-746.00000423
ACGTTCC351.0098847E-746.00000424
GGACCAC351.0098847E-746.00000419
GAGCCTT351.0098847E-746.00000413
CCAGACC700.046.00000438
CGGATTA700.046.00000415
ACCAACC700.046.00000442
GCGGATT700.046.00000414
ACCTGTA351.0098847E-746.00000428
ATTACCT700.046.00000418
CTTGGCT351.0098847E-746.00000417
ACCTGAG700.046.00000421
Produced by FastQC (version 0.11.5)