##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527974_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	125776
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.83826803205699	31.0	31.0	33.0	30.0	33.0
2	31.549222427172115	31.0	31.0	34.0	30.0	34.0
3	31.90435377178476	33.0	31.0	34.0	30.0	34.0
4	35.716464190306574	37.0	35.0	37.0	33.0	37.0
5	35.922775410253145	37.0	35.0	37.0	35.0	37.0
6	35.95138182165119	37.0	35.0	37.0	35.0	37.0
7	35.869800279862616	37.0	35.0	37.0	35.0	37.0
8	34.3216511894161	35.0	35.0	36.0	32.0	37.0
9	37.041756773947334	39.0	37.0	39.0	33.0	39.0
10	35.85502003561888	37.0	35.0	39.0	32.0	39.0
11	37.774480027986264	39.0	37.0	39.0	35.0	39.0
12	38.18386655641776	39.0	39.0	39.0	37.0	39.0
13	38.13489854980283	39.0	39.0	39.0	37.0	39.0
14	38.57863185345376	40.0	39.0	40.0	36.0	40.0
15	37.723627719119705	40.0	37.0	40.0	33.0	40.0
16	36.62115983971505	38.0	35.0	40.0	31.0	40.0
17	36.37756805749905	38.0	35.0	40.0	31.0	40.0
18	36.427021053301104	38.0	35.0	40.0	31.0	40.0
19	36.68140185727007	39.0	35.0	40.0	31.0	40.0
20	36.741564368400965	39.0	35.0	40.0	32.0	40.0
21	36.942151125810966	39.0	35.0	40.0	32.0	40.0
22	37.033869736674724	39.0	35.0	40.0	32.0	40.0
23	36.90829729042107	39.0	35.0	40.0	32.0	40.0
24	37.02908344994275	39.0	35.0	40.0	32.0	40.0
25	36.73181688080397	39.0	35.0	40.0	32.0	40.0
26	36.6601895433151	39.0	35.0	40.0	32.0	40.0
27	36.291709070092864	38.0	35.0	40.0	31.0	40.0
28	35.996811792392826	38.0	35.0	40.0	30.0	40.0
29	35.94835262689225	38.0	35.0	40.0	30.0	40.0
30	35.89500381630836	38.0	35.0	40.0	30.0	40.0
31	35.73048912352118	38.0	35.0	40.0	30.0	40.0
32	35.425256010685665	37.0	35.0	40.0	29.0	40.0
33	35.04701214858161	36.0	34.0	40.0	26.0	40.0
34	34.798554573209515	36.0	34.0	40.0	25.0	40.0
35	34.44058484925582	35.0	33.0	40.0	24.0	40.0
36	33.7403161175423	35.0	33.0	40.0	21.0	40.0
37	33.758364075817326	35.0	33.0	40.0	21.0	40.0
38	33.46659935122758	35.0	33.0	40.0	21.0	40.0
39	33.03922051901794	35.0	33.0	40.0	18.0	40.0
40	32.45385447144129	35.0	33.0	39.0	15.0	40.0
41	31.82350368909808	35.0	31.0	39.0	12.0	40.0
42	30.978493512275794	35.0	30.0	38.0	10.0	40.0
43	30.23770830683119	35.0	26.0	38.0	9.0	40.0
44	29.500604248823304	35.0	23.0	37.0	8.0	40.0
45	28.644113344358225	35.0	20.0	36.0	8.0	40.0
46	28.12290898104567	34.0	20.0	36.0	8.0	40.0
47	27.685385129118433	34.0	17.0	35.0	8.0	40.0
48	27.16018159267269	33.0	15.0	35.0	8.0	40.0
49	26.76012911843277	33.0	12.0	35.0	8.0	40.0
50	26.200976338888182	33.0	10.0	35.0	8.0	40.0
51	25.804819679430096	33.0	10.0	35.0	8.0	40.0
52	25.013015201628292	32.0	10.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	4.0
16	20.0
17	48.0
18	116.0
19	235.0
20	350.0
21	616.0
22	838.0
23	1132.0
24	1665.0
25	2228.0
26	2898.0
27	3923.0
28	4968.0
29	6034.0
30	6986.0
31	7189.0
32	6926.0
33	6621.0
34	7802.0
35	10770.0
36	13564.0
37	19712.0
38	16767.0
39	4362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.000667853962604	24.507060170461774	30.81112453886274	3.6811474367128865
2	33.2050629690879	21.353040325658313	28.508618496374506	16.933278208879276
3	23.038576516982573	19.67783996946953	36.51412034092355	20.76946317262435
4	30.293537717847602	25.434900139931305	23.720741635924185	20.550820506296912
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	100.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	97.71737056354154	1.4454267904846712	0.5191769494975195	0.3180256964762753
12	97.58857015646865	0.8698002798626129	1.2728978501462918	0.2687317135224526
13	95.67405546368147	2.302506042488233	1.4311156341432387	0.5923228596870628
14	59.37062714667345	23.481427299325787	9.11382139676886	8.034124157231904
15	40.80667217911208	34.574163592418266	14.27855870754357	10.340605520926092
16	45.39180765805877	25.544619005215623	19.420239155323753	9.643334181401856
17	31.083831573591148	27.72309502607811	21.965239791375144	19.227833608955603
18	25.138341177967177	36.65564177585549	21.125651952677778	17.080365093499555
19	28.612771911970487	39.09092354662257	22.716575499300344	9.579729042106603
20	21.044555400076327	38.87864139422466	24.209706144256458	15.867097061442564
21	22.384238646482636	39.67291057117415	19.747805622694315	18.195045159648902
22	24.70026078107111	38.566976211677904	16.933278208879276	19.799484798371708
23	17.79433278208879	40.157899758300466	27.58554891235212	14.462218547258617
24	25.687730568629945	42.54945299580206	17.500159012848236	14.262657422719755
25	24.783742526396132	39.373171352245265	25.46034219564941	10.382743925709196
26	27.24923673832846	44.13322096425391	17.418267396005596	11.199274901412034
27	24.53568248314464	43.92968451850909	22.567898486197684	8.966734512148582
28	32.58570792520036	43.579061188144	12.54531866174787	11.289912224907772
29	31.61811474367129	44.19762116779036	12.045223254038927	12.139040834499427
30	34.77372471695713	47.100400712377564	9.94625365729551	8.179620913369801
31	29.938144002035365	49.36553873552983	11.489473349446635	9.20684391298817
32	26.816721791120724	54.52471059661621	9.239441546876987	9.419126065386083
33	22.696698893270575	56.68251494720773	10.095725734639359	10.52506042488233
34	17.972427172115506	61.03310647500319	12.53577789085358	8.458688462027732
35	21.198002798626128	58.92459610736548	11.44733494466353	8.430066149344867
36	16.204204299707417	60.414546495356824	14.164069456812111	9.217179748123648
37	15.909235466225672	62.48091845821142	12.803714540134841	8.806131535428062
38	16.226466098460758	62.79258364075817	11.157931560870118	9.823018699910953
39	15.068057499045922	63.40239791375143	11.925963617860322	9.603580969342323
40	15.301011321714794	60.19828902175296	11.347156850273501	13.153542806258745
41	16.720200992240173	58.071492176567865	14.575912733748886	10.632394097443074
42	19.24691515074418	56.21342704490523	14.385892380104313	10.153765424246279
43	22.059057371835642	56.22058262307594	11.90131662638341	9.819043378704999
44	21.807817071619386	54.67179748123648	12.908663019972014	10.611722427172115
45	22.994847983717083	53.51179875333927	12.618464571937412	10.874888691006234
46	22.235561633379977	51.1790802696858	14.08217783996947	12.503180256964763
47	26.136146800661493	48.991858542170206	12.141426027223	12.7305686299453
48	24.45617605902557	50.72191833100115	12.178794046558963	12.643111563414324
49	24.186649281261925	50.818121104185224	13.147182292329221	11.848047322223636
50	24.979328329729043	48.85669762116779	13.341177967179748	12.822796081923418
51	24.968992494593564	49.81156977483781	12.721822923292203	12.497614807276427
52	23.11808294110164	48.82250985879659	12.942055718102022	15.117351481999746
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	9.5
12	18.0
13	44.0
14	140.5
15	211.0
16	231.0
17	251.0
18	359.0
19	467.0
20	558.0
21	649.0
22	990.5
23	1332.0
24	1731.5
25	2131.0
26	2949.0
27	3767.0
28	4704.0
29	5641.0
30	6276.0
31	6911.0
32	7269.5
33	7628.0
34	7661.5
35	7695.0
36	8112.0
37	8529.0
38	8334.0
39	7692.0
40	7245.0
41	6659.0
42	6073.0
43	6242.5
44	6412.0
45	6751.5
46	7091.0
47	6404.5
48	5718.0
49	6247.0
50	6776.0
51	6729.5
52	6683.0
53	6289.0
54	5895.0
55	5963.5
56	6032.0
57	5395.5
58	4759.0
59	4251.5
60	3744.0
61	2754.5
62	1765.0
63	1511.0
64	1542.0
65	1827.0
66	1058.0
67	289.0
68	458.0
69	627.0
70	349.5
71	72.0
72	50.0
73	28.0
74	23.0
75	18.0
76	16.0
77	14.0
78	9.0
79	4.0
80	4.5
81	5.0
82	3.5
83	2.0
84	1.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	125776.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.264088538353896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.31955996780252	51.74914133061952
2	4.847061980144889	5.745134206843913
3	2.0150254896699757	3.582559470805241
4	1.2557016367051248	2.976720519017937
5	0.9109203112422861	2.6992430988423863
6	0.713710759323853	2.5378450578806766
7	0.5030855916286557	2.0870436331255564
8	0.4172256506573652	1.9781198320824323
9	0.3394150791521331	1.810361277191197
>10	1.6004829621679637	14.796145528558707
>50	0.028172793131204722	1.2450706017046178
>100	0.042929970485645286	5.744339142602723
>500	0.005366246310705661	1.9979964381121995
>1k	0.0013415615776764152	1.050279862612899
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATCCTGTGTGGGGGTCTGTGTGGGAGGCAGGCAGATCACTTGAGCCCAGGA	1321	1.050279862612899	No Hit
AAGACTGTGTGGGGACTTGGAGCAGCTCTGCCGCGGGGACTGCACTGTCCGC	826	0.6567230632235085	No Hit
GCTCCTGTGTATAAGAATGAACTAGAGTTGTCATTTTTCTCTTCACTGGATG	588	0.4674977738201247	No Hit
GAGGCTGTGTGGGTCCCACCAGAAATTGAGGAGTCCCATCTGAGTCTTTTGC	558	0.44364584658440404	No Hit
AATGCTGTGTGGGGCGGATTACCTGAGGTCAGGAGTTCCAGACCAACCTGAC	541	0.4301297544841623	No Hit
TGGCCTGTGTGGGCCCCCTTCTGGAGGGCTTCCACTGCCCTTCCAGGCTGGG	490	0.3895814781834372	No Hit
CGTGCTGTGTGGTCGGGGACAGTGGCTCATGCCTGTAATCCCAGCAGTTTAG	478	0.38004070728914896	No Hit
ATGGCTGTGTGGGGCAGAGTCTGCAAAAAGAGAGATTCAAACTGCTGAATCA	473	0.3760653860831955	No Hit
CTGTCTGTGTGGGGGCACTGTGCTAAACAATCTACAAAGCTTCTTCCAGCTC	413	0.32836153161175424	No Hit
AGTGCTGTGTGGGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC	304	0.24169952932196923	No Hit
AGTTCTGTGTGGGGGCGGGCGGATAATGAGGTCAGGAGATCGAGACCATTGT	277	0.22023279480982064	No Hit
GTCGCTGTGTAGGGTTCCGATGTTACCGGTTGGCGGTATCACACCGGAAACC	276	0.21943773056862995	No Hit
GTGCCTGTGTGGTGCAGATATGGCTGCAGTGACCAAAAACTTAGATCACAAC	274	0.21784760208624857	No Hit
GCTTCTGTGTGGGGGTGGGGACCACGTTCCAGGCGCCGGTGGACAGGTACAT	267	0.21228215239791373	No Hit
GTACCTGTGTGGGTTGGGAGGGCCACCCAACCCTTCCCTGATGAACTCCCCC	257	0.20433150998600685	No Hit
GAAGCTGTGTGGGTATGTGGTGTGTGTGTGTGGGTGTGTGTTGTGTGTGTGT	255	0.2027413815036255	No Hit
TGTCCTGTGTGGGAGCCTTGGCTTTAACCCAACTAAATCAGAAGTGTTTGGT	250	0.19876606029767208	No Hit
GACTCTGTGTTTGTCTGTTTAGTTCTGTGTGTAAATGAAATGTGGAAATGAC	249	0.19797099605648136	No Hit
GCGTCTGTGTGGGGAGGCTGGGAAATACTAAATTTAATCAGTGTAGCCAGGG	245	0.1947907390917186	No Hit
TTTTCTGTGTGGGGCGGACCCAGGTCTACTTGCTTCCTTCCCATTACACAAA	228	0.1812746469914769	No Hit
GCGGCTGTGTGGGTGGGAAGCTATACTATATGGGAGGCTCTCTCATTGGTTT	221	0.1757091973031421	No Hit
TTGCCTGTGTGGGGAAGGGATCCAGTTTCAGCTTTCTACATATGTCTAGCCA	205	0.16298816944409109	No Hit
GGTTCTGTGTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT	183	0.14549675613789592	No Hit
CATCCTGTGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG	179	0.1423164991731332	No Hit
CGGACTGTGTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC	168	0.13357079252003562	No Hit
GGCCCTGTGTGAGGAACGATTGGTGTTGCTGCTGGTGGTTGGGAGTAGTTGT	164	0.13039053555527286	No Hit
ATCACTGTGTGGGGGGAATTCACTGTCCTTTCATCCATAAGCGGAGAAAGAG	147	0.11687444345503117	No Hit
GTTGCTGTGTGGGAGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGA	143	0.11369418649026843	No Hit
GATGCTGTGTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT	141	0.11210405800788703	No Hit
GGGCCTGTGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG	135	0.1073336725607429	No Hit
AAGGCTGTGTGGGGTGGTATCAACGCAGATTATTCCTGTGTGGGATAAAACA	130	0.10335835135478948	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.003975321205953441	0.0	0.0	0.0	0.0
12	0.03975321205953441	0.0	0.0	0.0	0.0
13	0.07235084594835263	0.0	0.0	0.0	0.0
14	0.19240554636814655	0.0	0.0	0.0	0.0
15	0.3784505788067676	0.0	0.0	0.0	0.0
16	0.5008904719501336	0.0	0.0	0.0	0.0
17	0.5994784378577789	0.0	0.0	0.0	0.0
18	0.7314591018954332	0.0	0.0	0.0	0.0
19	0.8912670143747615	0.0	0.0	0.0	0.0
20	1.0868528177076708	0.0	0.0	0.0	0.0
21	1.267332400457957	0.0	0.0	0.0	0.0
22	1.4470169189670525	0.0	0.0	0.0	0.0
23	1.6020544459992367	0.0	0.0	0.0	0.0
24	1.777763643302379	0.0	0.0	0.0	0.0
25	1.9868655387355298	0.0	0.0	0.0	0.0
26	2.236515710469406	0.0	0.0	0.0	0.0
27	2.5139931306449563	0.0	0.0	0.0	0.0
28	2.83042869863885	0.0	0.0	0.0	0.0
29	3.1969533138277573	0.0	0.0	0.0	0.0
30	3.5857397277700036	0.0	0.0	0.0	0.0
31	3.988042233812492	0.0	0.0	0.0	0.0
32	4.3434359496247295	0.0	0.0	0.0	0.0
33	4.665436967306959	0.0	0.0	0.0	0.0
34	5.022420811601577	0.0	0.0	0.0	0.0
35	5.365093499554764	0.0	0.0	0.0	0.0
36	5.656882076071747	0.0	0.0	0.0	0.0
37	5.932769367764916	0.0	0.0	0.0	0.0
38	6.224557944281898	0.0	0.0	0.0	0.0
39	6.517141585040071	0.0	0.0	0.0	0.0
40	6.776332527668235	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAACTA	35	1.0098847E-7	46.000004	29
TCCCATT	35	1.0098847E-7	46.000004	39
GTTTGGT	35	1.0098847E-7	46.000004	46
CCAACCT	70	0.0	46.000004	43
CTGAGGT	70	0.0	46.000004	23
TAAATCA	35	1.0098847E-7	46.000004	34
ACTGATG	35	1.0098847E-7	46.000004	41
CTTTAAC	35	1.0098847E-7	46.000004	22
AAATTGA	35	1.0098847E-7	46.000004	23
ACGTTCC	35	1.0098847E-7	46.000004	24
GGACCAC	35	1.0098847E-7	46.000004	19
GAGCCTT	35	1.0098847E-7	46.000004	13
CCAGACC	70	0.0	46.000004	38
CGGATTA	70	0.0	46.000004	15
ACCAACC	70	0.0	46.000004	42
GCGGATT	70	0.0	46.000004	14
ACCTGTA	35	1.0098847E-7	46.000004	28
ATTACCT	70	0.0	46.000004	18
CTTGGCT	35	1.0098847E-7	46.000004	17
ACCTGAG	70	0.0	46.000004	21
>>END_MODULE