SRR1527973_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527973_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	130168
Sequences flagged as poor quality	0
Sequence length	59
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATCCTGTGTGGGGGTCTGTGTGGGAGGCAGGCAGATCACTTGAGCCCAGGAGTTGGAA	1282	0.9848810767623379	No Hit
AAGACTGTGTGGGGACTTGGAGCAGCTCTGCCGCGGGGACTGCACTGTCCGCCCTGCCA	913	0.7014012660561736	No Hit
GCTCCTGTGTATAAGAATGAACTAGAGTTGTCATTTTTCTCTTCACTGGATGTTTATTT	576	0.44250507037059794	No Hit
AATGCTGTGTGGGGCGGATTACCTGAGGTCAGGAGTTCCAGACCAACCTGACCAACATA	564	0.4332862147378772	No Hit
GAGGCTGTGTGGGTCCCACCAGAAATTGAGGAGTCCCATCTGAGTCTTTTGCACTGAAC	541	0.41561674144182903	No Hit
CTGTCTGTGTGGGGGCACTGTGCTAAACAATCTACAAAGCTTCTTCCAGCTCACTGCAG	493	0.37874131891094587	No Hit
CGTGCTGTGTGGTCGGGGACAGTGGCTCATGCCTGTAATCCCAGCAGTTTAGGAGGCTG	453	0.3480118001352099	No Hit
ATGGCTGTGTGGGGCAGAGTCTGCAAAAAGAGAGATTCAAACTGCTGAATCAAAAGGTA	446	0.34263413434945605	No Hit
TGGCCTGTGTGGGCCCCCTTCTGGAGGGCTTCCACTGCCCTTCCAGGCTGGGCCTAGCA	403	0.3095999016655399	No Hit
GCTTCTGTGTGGGGGTGGGGACCACGTTCCAGGCGCCGGTGGACAGGTACATCATCGGG	323	0.24814086411406797	No Hit
AGTGCTGTGTGGGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGG	292	0.22432548706287259	No Hit
GTCGCTGTGTAGGGTTCCGATGTTACCGGTTGGCGGTATCACACCGGAAACCATGGCGC	291	0.22355724909347918	No Hit
GTACCTGTGTGGGTTGGGAGGGCCACCCAACCCTTCCCTGATGAACTCCCCCATACACT	281	0.2158748693995452	No Hit
GACTCTGTGTTTGTCTGTTTAGTTCTGTGTGTAAATGAAATGTGGAAATGACCCTCCCT	279	0.2143383934607584	No Hit
GTGCCTGTGTGGTGCAGATATGGCTGCAGTGACCAAAAACTTAGATCACAACATTCAAG	277	0.2128019175219716	No Hit
TGTCCTGTGTGGGAGCCTTGGCTTTAACCCAACTAAATCAGAAGTGTTTGGTTTAGTCA	267	0.20511953782803763	No Hit
GCGTCTGTGTGGGGAGGCTGGGAAATACTAAATTTAATCAGTGTAGCCAGGGTGGATCT	239	0.18360887468502243	No Hit
TTTTCTGTGTGGGGCGGACCCAGGTCTACTTGCTTCCTTCCCATTACACAAAATTGTCA	230	0.17669473296048183	No Hit
AGTTCTGTGTGGGGGCGGGCGGATAATGAGGTCAGGAGATCGAGACCATTGTGGTTAAC	221	0.16978059123594125	No Hit
GAAGCTGTGTGGGTATGTGGTGTGTGTGTGTGGGTGTGTGTTGTGTGTGTGTGTGTGTG	202	0.15518406981746666	No Hit
TTGCCTGTGTGGGGAAGGGATCCAGTTTCAGCTTTCTACATATGTCTAGCCAGTTTTCC	198	0.15211111793989304	No Hit
GGCCCTGTGTGAGGAACGATTGGTGTTGCTGCTGGTGGTTGGGAGTAGTTGTGGAAAGC	192	0.14750169012353265	No Hit
GGTTCTGTGTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGAC	167	0.12829574088869766	No Hit
CGGACTGTGTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGG	165	0.12675926494991088	No Hit
GCGGCTGTGTGGGTGGGAAGCTATACTATATGGGAGGCTCTCTCATTGGTTTATACTTC	164	0.12599102698051748	No Hit
GATGCTGTGTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGAC	151	0.1160039333784033	No Hit
CATCCTGTGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCAT	150	0.11523569540900991	No Hit
ATCACTGTGTGGGGGGAATTCACTGTCCTTTCATCCATAAGCGGAGAAAGAGGGAATGA	149	0.1144674574396165	No Hit
AAGGCTGTGTGGGGTGGTATCAACGCAGATTATTCCTGTGTGGGATAAAACATATTAAT	146	0.11216274353143629	No Hit
TCGCCTGTGTGGGGCGCAGTGGCTCACACCTGTAATCTTAACACTTTGGGAGGCCGAGG	139	0.1067850777456825	No Hit
GTTGCTGTGTGGGAGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCA	135	0.10371212586810892	No Hit
AAATCTGTGTGGAGAATCTGCCGCGAATGCCGCCTGAGAAAAAAGAGAAGCAATGCCGC	133	0.10217564992932211	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCCAG	50	3.6379788E-12	53.000004	38
TGGTCGG	50	3.6379788E-12	53.000004	10
CCAACTA	25	1.47143E-5	53.000004	29
GTTTGGT	25	1.47143E-5	53.000004	46
CAGTTTA	50	3.6379788E-12	53.000004	45
AATCCCA	50	3.6379788E-12	53.000004	37
GCCTTGG	25	1.47143E-5	53.000004	15
TAGGAGG	50	3.6379788E-12	53.000004	50
CCCAGCA	50	3.6379788E-12	53.000004	40
TAAATCA	25	1.47143E-5	53.000004	34
CAGCAGT	50	3.6379788E-12	53.000004	42
CTTTAAC	25	1.47143E-5	53.000004	22
GAAATGT	25	1.47143E-5	53.000004	37
GTGTAAA	25	1.47143E-5	53.000004	29
CAACTAA	25	1.47143E-5	53.000004	30
TGTTTGT	25	1.47143E-5	53.000004	8
TGTTTAG	25	1.47143E-5	53.000004	16
GGTTTAG	25	1.47143E-5	53.000004	50
CTGACCA	75	0.0	53.000004	48
GGTCAAC	50	3.6379788E-12	53.000004	12

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content