##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527973_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	130168
Sequences flagged as poor quality	0
Sequence length	59
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.61642646426157	31.0	31.0	33.0	28.0	33.0
2	31.316729150021512	31.0	31.0	33.0	30.0	34.0
3	31.673660192981377	31.0	31.0	34.0	30.0	34.0
4	35.575279638620856	37.0	35.0	37.0	33.0	37.0
5	35.74819464077193	37.0	35.0	37.0	35.0	37.0
6	35.74052762583738	37.0	35.0	37.0	33.0	37.0
7	35.67697129862946	37.0	35.0	37.0	33.0	37.0
8	33.92548091696884	35.0	33.0	35.0	30.0	37.0
9	36.66140679736955	37.0	35.0	39.0	32.0	39.0
10	35.05558969946531	37.0	34.0	37.0	30.0	39.0
11	37.44710681580727	39.0	37.0	39.0	35.0	39.0
12	37.99320877635056	39.0	37.0	39.0	35.0	39.0
13	37.945332186097964	39.0	38.0	39.0	35.0	39.0
14	37.97017700202815	40.0	38.0	40.0	33.0	40.0
15	37.03437096675066	39.0	36.0	40.0	31.0	40.0
16	36.0130369983406	38.0	35.0	40.0	31.0	40.0
17	35.55580480609674	38.0	34.0	40.0	27.0	40.0
18	35.5918198021019	38.0	34.0	40.0	27.0	40.0
19	35.84047538565546	38.0	34.0	40.0	29.0	40.0
20	36.03183578145166	38.0	34.0	40.0	30.0	40.0
21	36.240635179153095	38.0	35.0	40.0	30.0	40.0
22	36.38047753672178	38.0	35.0	40.0	30.0	40.0
23	36.26389742486633	38.0	35.0	40.0	30.0	40.0
24	36.39598057894413	38.0	35.0	40.0	31.0	40.0
25	35.982591727613546	38.0	35.0	40.0	30.0	40.0
26	35.75866572429476	38.0	35.0	40.0	29.0	40.0
27	35.3339684100547	38.0	34.0	40.0	27.0	40.0
28	35.300319586995265	37.0	34.0	40.0	27.0	40.0
29	35.1073919857415	37.0	34.0	40.0	27.0	40.0
30	34.978566160653926	37.0	33.0	40.0	26.0	40.0
31	34.793221068158076	36.0	33.0	40.0	26.0	40.0
32	34.36240857968164	35.0	33.0	40.0	24.0	40.0
33	33.911898469669964	35.0	33.0	40.0	22.0	40.0
34	33.655468317866145	35.0	33.0	40.0	21.0	40.0
35	33.33716428000738	35.0	33.0	39.0	21.0	40.0
36	32.90115850285785	35.0	32.0	39.0	20.0	40.0
37	32.75198205396104	35.0	32.0	39.0	19.0	40.0
38	32.40377051195378	35.0	31.0	39.0	18.0	40.0
39	31.970023354434268	35.0	31.0	39.0	15.0	40.0
40	31.383665724294758	35.0	30.0	38.0	15.0	40.0
41	30.75513951201524	35.0	28.0	38.0	10.0	40.0
42	30.117256161268514	35.0	25.0	37.0	10.0	40.0
43	29.299843279454244	34.0	23.0	36.0	9.0	40.0
44	28.62337901788458	33.0	21.0	35.0	8.0	40.0
45	27.963370413619323	33.0	20.0	35.0	8.0	40.0
46	27.265925573105527	33.0	18.0	35.0	8.0	39.0
47	26.79321338577838	33.0	15.0	35.0	8.0	39.0
48	26.206632966627744	33.0	12.0	35.0	8.0	39.0
49	25.812173498863007	33.0	10.0	35.0	8.0	39.0
50	25.313318173437406	31.0	10.0	35.0	8.0	39.0
51	24.841289717903017	31.0	10.0	35.0	8.0	38.0
52	24.33151004855264	30.0	10.0	35.0	8.0	38.0
53	23.858252412267223	28.0	9.0	35.0	8.0	38.0
54	23.292345276872965	27.0	9.0	35.0	8.0	37.0
55	22.77598949050458	25.0	9.0	35.0	8.0	37.0
56	22.18317866142216	23.0	8.0	35.0	8.0	37.0
57	21.585074672730624	22.0	8.0	34.0	8.0	36.0
58	20.955388421117327	20.0	8.0	34.0	8.0	36.0
59	20.233275459406304	18.0	8.0	33.0	6.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	24.0
16	66.0
17	178.0
18	395.0
19	683.0
20	1132.0
21	1635.0
22	2263.0
23	3021.0
24	4107.0
25	5083.0
26	6094.0
27	7241.0
28	7796.0
29	7082.0
30	6564.0
31	6569.0
32	7169.0
33	8162.0
34	9490.0
35	10805.0
36	11561.0
37	11893.0
38	9803.0
39	1349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.02698051748509	25.075287321000555	30.283940753487798	3.6137914080265503
2	32.72770573412821	21.559062135086965	28.586134841128384	17.127097289656444
3	22.608475201278345	20.07559461618831	36.23701677831725	21.07891340421609
4	30.213262860303608	25.546985434208104	23.717042591113024	20.522709114375267
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	100.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	97.7521357015549	1.415862577592035	0.5185606293405446	0.31344109151250693
12	97.61923053284985	0.8304652449142645	1.2844938848257637	0.26581033741011617
13	95.74703460143814	2.2578513920472005	1.3720730133366112	0.6230409931780468
14	59.12897179030176	23.721652018929383	9.025259664433655	8.124116526335197
15	40.22801302931596	35.053930305451416	14.21317067174728	10.504885993485342
16	45.12092065638252	25.284248048675558	19.782895949849426	9.811935345092495
17	30.67727859381722	27.63736094892754	22.370321430766392	19.315039026488844
18	24.992317620306064	36.586565054391244	21.05125683731793	17.36986048798476
19	28.5500276565669	38.94736033433717	22.987216520189293	9.515395488906643
20	20.9728965644398	38.69998770819249	24.394628480118	15.932487247249707
21	22.275059922561613	39.76860672361871	19.86048798475816	18.095845369061518
22	24.756468563702292	38.406520803884206	16.862823428185113	19.974187204228382
23	17.777794849732654	39.98294511707947	27.628142093294816	14.611117939893061
24	25.754409685944317	42.53119046155737	17.61262368631307	14.101776166185237
25	24.530606600700633	38.99422285047016	25.77515211111794	10.700018437711266
26	27.6066314301518	43.875606907995824	17.24540593694303	11.272355724909348
27	24.149560567881505	44.026949787966316	22.765195747034603	9.05829389711757
28	32.33820908364575	43.62669780591236	12.568373179276012	11.466719931165878
29	31.742824657365865	44.108383012722015	11.913066191383443	12.23572613852867
30	34.8119353450925	46.925511646487614	10.029346690430827	8.23320631798906
31	29.97818204166923	49.364667199311654	11.274660438817529	9.382490320201585
32	26.912912543789563	54.77690369368816	8.983006576117019	9.327177186405262
33	22.323458914633395	57.21375453260402	10.077745682502611	10.385040870259973
34	17.99136500522402	61.36608075717535	12.311013459529224	8.331540778071416
35	21.082754594063058	59.39708684162006	11.145596459959437	8.374562104357446
36	15.768852559768915	61.69565484604511	14.068741933501322	8.466750660684653
37	15.772693749615883	63.065423145473545	12.441613914326103	8.720269190584476
38	15.991641570893	63.42419027718026	10.934331018376252	9.64983713355049
39	14.75093725032266	64.19242824657367	11.687204228381784	9.369430274721898
40	14.79165386270051	61.17171655091881	11.090283326163112	12.946346260217565
41	16.276657857537952	59.03524675803576	14.297676848380556	10.390418536045726
42	18.471513736094895	57.75766701493454	13.88359658287751	9.887222666093049
43	21.380062688218302	57.29826070923729	11.585796816421855	9.735879786122549
44	20.736279269866635	56.010693872533956	12.735080818634382	10.51794603896503
45	22.033833200172083	55.26550304222236	12.251090897916539	10.449572859689017
46	21.26021756499293	52.50676049413067	14.058754839899207	12.174267100977199
47	25.359535369676113	50.522401819187515	11.63880523631	12.479257574826379
48	23.153155921578268	52.38230594308894	11.809354065515334	12.655184069817466
49	23.150082969700694	52.17949111916907	12.83418351668613	11.836242394444103
50	23.511154815315592	50.487831110564805	12.85492594185975	13.146088132259848
51	23.85455718763444	51.15466166799828	12.518437711265443	12.472343433101837
52	21.63972712187327	50.17054882920533	12.606785077745682	15.582938971175711
53	22.348810767623377	49.998463524061215	12.589115604449635	15.063610103865773
54	20.069448712433164	48.375176694732964	15.897148300657612	15.658226292176264
55	20.803884211173255	47.33728719808248	17.945270727060414	13.913557863683854
56	25.665294081494682	44.863560936635736	13.428799704996619	16.042345276872965
57	23.82766885870567	43.56677524429968	15.055159486202447	17.550396410792207
58	22.58389158625776	42.911468256407105	16.36884641386516	18.13579374346998
59	23.08631921824104	41.500983344600826	17.642584967119415	17.77011247003872
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	5.5
10	9.0
11	25.5
12	42.0
13	87.5
14	145.0
15	157.0
16	188.5
17	220.0
18	220.0
19	282.0
20	344.0
21	421.0
22	498.0
23	702.5
24	907.0
25	1314.0
26	2312.5
27	2904.0
28	3724.5
29	4545.0
30	5419.0
31	7056.5
32	7820.0
33	8635.5
34	9451.0
35	9201.0
36	8592.0
37	8233.0
38	8102.5
39	7972.0
40	7732.5
41	7493.0
42	6804.5
43	6752.5
44	7389.0
45	6994.0
46	6599.0
47	6237.0
48	5778.5
49	5682.0
50	5517.5
51	5353.0
52	5404.5
53	4792.0
54	4128.0
55	3824.5
56	3521.0
57	3812.0
58	3478.0
59	2853.0
60	1982.0
61	1111.0
62	1051.5
63	992.0
64	785.0
65	1141.0
66	1704.0
67	1130.5
68	557.0
69	325.0
70	72.0
71	51.0
72	45.5
73	40.0
74	33.0
75	16.5
76	7.0
77	6.5
78	6.0
79	5.5
80	5.0
81	6.0
82	3.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	130168.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.15294081494685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.72137977003833	63.428031467027225
2	3.513858801310893	4.859873394382644
3	1.4008776315058602	2.90624423821523
4	0.8554129867244348	2.3661729457316696
5	0.5743487196578348	1.9858951508819371
6	0.40882075209687274	1.6962694364206257
7	0.2532911181469755	1.2261077991518652
8	0.23996000666555573	1.327515211111794
9	0.1510859301227573	0.9403232745375207
>10	0.8165305782369604	9.467764734804254
>50	0.019996667222129644	1.04634011431381
>100	0.03888240848747431	5.771771864052609
>500	0.004443703827139921	1.9928092926064778
>1k	0.0011109259567849803	0.9848810767623379
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATCCTGTGTGGGGGTCTGTGTGGGAGGCAGGCAGATCACTTGAGCCCAGGAGTTGGAA	1282	0.9848810767623379	No Hit
AAGACTGTGTGGGGACTTGGAGCAGCTCTGCCGCGGGGACTGCACTGTCCGCCCTGCCA	913	0.7014012660561736	No Hit
GCTCCTGTGTATAAGAATGAACTAGAGTTGTCATTTTTCTCTTCACTGGATGTTTATTT	576	0.44250507037059794	No Hit
AATGCTGTGTGGGGCGGATTACCTGAGGTCAGGAGTTCCAGACCAACCTGACCAACATA	564	0.4332862147378772	No Hit
GAGGCTGTGTGGGTCCCACCAGAAATTGAGGAGTCCCATCTGAGTCTTTTGCACTGAAC	541	0.41561674144182903	No Hit
CTGTCTGTGTGGGGGCACTGTGCTAAACAATCTACAAAGCTTCTTCCAGCTCACTGCAG	493	0.37874131891094587	No Hit
CGTGCTGTGTGGTCGGGGACAGTGGCTCATGCCTGTAATCCCAGCAGTTTAGGAGGCTG	453	0.3480118001352099	No Hit
ATGGCTGTGTGGGGCAGAGTCTGCAAAAAGAGAGATTCAAACTGCTGAATCAAAAGGTA	446	0.34263413434945605	No Hit
TGGCCTGTGTGGGCCCCCTTCTGGAGGGCTTCCACTGCCCTTCCAGGCTGGGCCTAGCA	403	0.3095999016655399	No Hit
GCTTCTGTGTGGGGGTGGGGACCACGTTCCAGGCGCCGGTGGACAGGTACATCATCGGG	323	0.24814086411406797	No Hit
AGTGCTGTGTGGGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGG	292	0.22432548706287259	No Hit
GTCGCTGTGTAGGGTTCCGATGTTACCGGTTGGCGGTATCACACCGGAAACCATGGCGC	291	0.22355724909347918	No Hit
GTACCTGTGTGGGTTGGGAGGGCCACCCAACCCTTCCCTGATGAACTCCCCCATACACT	281	0.2158748693995452	No Hit
GACTCTGTGTTTGTCTGTTTAGTTCTGTGTGTAAATGAAATGTGGAAATGACCCTCCCT	279	0.2143383934607584	No Hit
GTGCCTGTGTGGTGCAGATATGGCTGCAGTGACCAAAAACTTAGATCACAACATTCAAG	277	0.2128019175219716	No Hit
TGTCCTGTGTGGGAGCCTTGGCTTTAACCCAACTAAATCAGAAGTGTTTGGTTTAGTCA	267	0.20511953782803763	No Hit
GCGTCTGTGTGGGGAGGCTGGGAAATACTAAATTTAATCAGTGTAGCCAGGGTGGATCT	239	0.18360887468502243	No Hit
TTTTCTGTGTGGGGCGGACCCAGGTCTACTTGCTTCCTTCCCATTACACAAAATTGTCA	230	0.17669473296048183	No Hit
AGTTCTGTGTGGGGGCGGGCGGATAATGAGGTCAGGAGATCGAGACCATTGTGGTTAAC	221	0.16978059123594125	No Hit
GAAGCTGTGTGGGTATGTGGTGTGTGTGTGTGGGTGTGTGTTGTGTGTGTGTGTGTGTG	202	0.15518406981746666	No Hit
TTGCCTGTGTGGGGAAGGGATCCAGTTTCAGCTTTCTACATATGTCTAGCCAGTTTTCC	198	0.15211111793989304	No Hit
GGCCCTGTGTGAGGAACGATTGGTGTTGCTGCTGGTGGTTGGGAGTAGTTGTGGAAAGC	192	0.14750169012353265	No Hit
GGTTCTGTGTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGAC	167	0.12829574088869766	No Hit
CGGACTGTGTGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGG	165	0.12675926494991088	No Hit
GCGGCTGTGTGGGTGGGAAGCTATACTATATGGGAGGCTCTCTCATTGGTTTATACTTC	164	0.12599102698051748	No Hit
GATGCTGTGTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGAC	151	0.1160039333784033	No Hit
CATCCTGTGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCAT	150	0.11523569540900991	No Hit
ATCACTGTGTGGGGGGAATTCACTGTCCTTTCATCCATAAGCGGAGAAAGAGGGAATGA	149	0.1144674574396165	No Hit
AAGGCTGTGTGGGGTGGTATCAACGCAGATTATTCCTGTGTGGGATAAAACATATTAAT	146	0.11216274353143629	No Hit
TCGCCTGTGTGGGGCGCAGTGGCTCACACCTGTAATCTTAACACTTTGGGAGGCCGAGG	139	0.1067850777456825	No Hit
GTTGCTGTGTGGGAGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCA	135	0.10371212586810892	No Hit
AAATCTGTGTGGAGAATCTGCCGCGAATGCCGCCTGAGAAAAAAGAGAAGCAATGCCGC	133	0.10217564992932211	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.00998709360211419	0.0	0.0	0.0	0.0
12	0.03533894659209637	0.0	0.0	0.0	0.0
13	0.05684960973511155	0.0	0.0	0.0	0.0
14	0.17899944686866204	0.0	0.0	0.0	0.0
15	0.37182717718640523	0.0	0.0	0.0	0.0
16	0.4640157335136132	0.0	0.0	0.0	0.0
17	0.5631184315653617	0.0	0.0	0.0	0.0
18	0.6614528916477168	0.0	0.0	0.0	0.0
19	0.8012722020773154	0.0	0.0	0.0	0.0
20	0.9725892692520435	0.0	0.0	0.0	0.0
21	1.1239321492225431	0.0	0.0	0.0	0.0
22	1.3067727859381721	0.0	0.0	0.0	0.0
23	1.4442873824595908	0.0	0.0	0.0	0.0
24	1.5971667383688772	0.0	0.0	0.0	0.0
25	1.796908610411161	0.0	0.0	0.0	0.0
26	2.045049474525229	0.0	0.0	0.0	0.0
27	2.3246880953844262	0.0	0.0	0.0	0.0
28	2.63736094892754	0.0	0.0	0.0	0.0
29	2.968471513736095	0.0	0.0	0.0	0.0
30	3.3741011615758096	0.0	0.0	0.0	0.0
31	3.736709483129494	0.0	0.0	0.0	0.0
32	4.044772908856247	0.0	0.0	0.0	0.0
33	4.352836334583	0.0	0.0	0.0	0.0
34	4.656290332493393	0.0	0.0	0.0	0.0
35	4.961280806342573	0.0	0.0	0.0	0.0
36	5.241687665171163	0.0	0.0	0.0	0.0
37	5.492901481162805	0.0	0.0	0.0	0.0
38	5.750261200909594	0.0	0.0	0.0	0.0
39	6.011462110503349	0.0	0.0	0.0	0.0
40	6.274967734005285	0.0	0.0	0.0	0.0
41	6.528486263905108	0.0	0.0	0.0	0.0
42	6.818111978366419	0.0	0.0	0.0	0.0
43	7.133089545817713	0.0	0.0	0.0	0.0
44	7.4488353512384	0.0	0.0	0.0	0.0
45	7.72693749615881	0.0	0.0	0.0	0.0
46	8.044219777518284	0.0	0.0	0.0	0.0
47	8.326931350255055	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCCAG	50	3.6379788E-12	53.000004	38
TGGTCGG	50	3.6379788E-12	53.000004	10
CCAACTA	25	1.47143E-5	53.000004	29
GTTTGGT	25	1.47143E-5	53.000004	46
CAGTTTA	50	3.6379788E-12	53.000004	45
AATCCCA	50	3.6379788E-12	53.000004	37
GCCTTGG	25	1.47143E-5	53.000004	15
TAGGAGG	50	3.6379788E-12	53.000004	50
CCCAGCA	50	3.6379788E-12	53.000004	40
TAAATCA	25	1.47143E-5	53.000004	34
CAGCAGT	50	3.6379788E-12	53.000004	42
CTTTAAC	25	1.47143E-5	53.000004	22
GAAATGT	25	1.47143E-5	53.000004	37
GTGTAAA	25	1.47143E-5	53.000004	29
CAACTAA	25	1.47143E-5	53.000004	30
TGTTTGT	25	1.47143E-5	53.000004	8
TGTTTAG	25	1.47143E-5	53.000004	16
GGTTTAG	25	1.47143E-5	53.000004	50
CTGACCA	75	0.0	53.000004	48
GGTCAAC	50	3.6379788E-12	53.000004	12
>>END_MODULE