FastQCFastQC Report
Fri 17 Jun 2016
SRR1527972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13558254
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA405.6188583E-946.00000423
ATGGGGC2353300.043.9739539
CAACTTA740700.043.7549633
ATATCGC1000.043.69999742
AACTTAT3127350.043.6267134
TATGGGC1196350.043.619938
CACTTAT3315850.043.616664
TATGGGG9374150.043.5923278
ATGGGAC435200.043.584799
TCACTTA1189300.043.4820483
ACACTTA796450.043.4298443
TTATGGG14066650.043.399587
ACTTATG14330850.043.3963245
CTTATGG14284750.043.3901756
GCACTTA851900.043.321753
TAACTTA1126350.043.2841453
ATGGGAG1103250.043.2460449
GACTTAT3048800.043.238914
ATGGGCT509950.043.2216879
AAACTTA720650.043.2169573
Produced by FastQC (version 0.11.5)