##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527972_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13558254
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.952522205292805	33.0	31.0	34.0	30.0	34.0
2	32.42723473096167	34.0	31.0	34.0	30.0	34.0
3	32.54533703233469	34.0	31.0	34.0	31.0	34.0
4	36.022701669403745	37.0	35.0	37.0	35.0	37.0
5	35.96428566687126	37.0	35.0	37.0	35.0	37.0
6	36.127845222548565	37.0	36.0	37.0	35.0	37.0
7	36.31025300160331	37.0	37.0	37.0	35.0	37.0
8	36.253232016452856	37.0	37.0	37.0	35.0	37.0
9	37.67452018526869	39.0	38.0	39.0	35.0	39.0
10	38.04647596954593	39.0	39.0	39.0	35.0	39.0
11	38.21227180136911	39.0	39.0	39.0	37.0	39.0
12	38.12496977855703	39.0	39.0	39.0	37.0	39.0
13	38.12087684741708	39.0	39.0	39.0	37.0	39.0
14	38.81111446945897	40.0	39.0	40.0	36.0	40.0
15	38.258299188081295	40.0	38.0	40.0	34.0	40.0
16	37.50129574206236	39.0	36.0	40.0	33.0	40.0
17	37.421093158455356	40.0	36.0	40.0	33.0	40.0
18	37.42369054304485	40.0	36.0	40.0	33.0	40.0
19	37.55828250451717	40.0	36.0	40.0	33.0	40.0
20	37.58309727786484	40.0	36.0	40.0	33.0	40.0
21	37.350858820022104	40.0	36.0	40.0	33.0	40.0
22	37.42097441160197	40.0	36.0	40.0	33.0	40.0
23	37.37201493643651	40.0	36.0	40.0	33.0	40.0
24	37.34884263121195	40.0	36.0	40.0	32.0	40.0
25	37.465460818184994	40.0	36.0	40.0	33.0	40.0
26	37.460961861313415	40.0	36.0	40.0	33.0	40.0
27	37.45613284719404	40.0	36.0	40.0	33.0	40.0
28	37.42301877513137	40.0	36.0	40.0	33.0	40.0
29	37.412790319461486	40.0	35.0	40.0	33.0	40.0
30	37.366135197054135	40.0	35.0	40.0	33.0	40.0
31	37.31956304993253	40.0	35.0	40.0	33.0	40.0
32	37.25395637225855	40.0	35.0	40.0	33.0	40.0
33	37.18182319050816	40.0	35.0	40.0	33.0	40.0
34	37.074393576046	40.0	35.0	40.0	33.0	40.0
35	36.97735976918562	39.0	35.0	40.0	32.0	40.0
36	36.89883277006022	39.0	35.0	40.0	32.0	40.0
37	36.76131432557614	39.0	35.0	40.0	32.0	40.0
38	36.52796289256714	39.0	35.0	40.0	31.0	40.0
39	36.39978444127098	39.0	35.0	40.0	31.0	40.0
40	36.29654496810578	39.0	35.0	40.0	31.0	40.0
41	36.144943220565125	38.0	35.0	40.0	31.0	40.0
42	35.9883519662635	38.0	35.0	40.0	31.0	40.0
43	35.817922278193045	38.0	35.0	40.0	30.0	40.0
44	35.65047188229399	37.0	35.0	40.0	30.0	40.0
45	35.47818480167137	37.0	35.0	40.0	30.0	40.0
46	35.34372560065625	37.0	35.0	40.0	30.0	40.0
47	35.176514542359214	36.0	34.0	40.0	29.0	40.0
48	35.034758752860064	36.0	34.0	40.0	29.0	40.0
49	34.87338863838957	36.0	34.0	40.0	28.0	40.0
50	34.71896868136561	35.0	34.0	39.0	28.0	40.0
51	34.547553173144564	35.0	34.0	39.0	28.0	40.0
52	33.89081949637468	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	2.0
13	10.0
14	23.0
15	56.0
16	193.0
17	543.0
18	1605.0
19	3459.0
20	6813.0
21	12000.0
22	19541.0
23	30434.0
24	44694.0
25	63186.0
26	87809.0
27	117719.0
28	153973.0
29	199378.0
30	251527.0
31	307980.0
32	374411.0
33	472465.0
34	724257.0
35	1511294.0
36	1108728.0
37	2098598.0
38	3946142.0
39	2021411.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.52256300848177	20.47474549451574	34.960334863176335	17.042356633826156
2	21.311320764458312	22.112876776021455	36.86050578488941	19.71529667463082
3	21.88200634093446	21.335726561841962	37.290207131390225	19.49205996583336
4	21.15312930411246	21.972040057665243	33.753918461772436	23.12091217644986
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.71112799627446	0.2213264333298373	0.05419576886522409	0.013349801530492054
12	99.62831497329965	0.26715829339087466	0.05679197336176177	0.04773475994770418
13	98.4492767284047	0.8560099257618273	0.20909034452371225	0.48562300130975566
14	66.43890135116219	19.457055458615837	5.444233453658561	8.659809736563425
15	37.838124289454974	24.443722620921545	15.200268412142155	22.517884677481334
16	31.822991367472536	16.990963585724238	24.989471358185206	26.19657368861802
17	24.448922405495573	16.57872761492741	28.032975337384887	30.93937464219213
18	23.390261017384688	17.62131023655406	31.582370414361616	27.40605833169964
19	26.44066854035925	17.34886365161768	28.196189568361824	28.014278239661245
20	30.248843250760753	17.296563407058166	24.575096468911113	27.879496873269964
21	31.98110169642787	18.27610693825326	21.343360288131496	28.39943107718737
22	31.605101954868232	19.55221520411109	21.850372474213863	26.992310366806816
23	29.997468700615876	20.988742355763506	20.819922683259954	28.19386626036066
24	30.334628632860838	22.978371698892794	19.63513148521926	27.051868183027107
25	31.090765816896482	20.919891307538567	22.425741544597113	25.56360133096784
26	30.524195814593824	20.038066848430482	21.88277340135389	27.5549639356218
27	32.13952917536432	20.07501850902041	20.969536342953894	26.815915972661376
28	33.35892659925091	19.954029479016988	19.52997782752853	27.157066094203575
29	31.657770978475547	18.722661487238696	19.005588772713654	30.613978761572103
30	29.707873889956627	18.20366398210271	20.795354623095275	31.293107504845384
31	27.964087411255168	18.47403065320948	20.824207895795432	32.73767403973993
32	29.131678754506297	22.232250553795495	19.585287309118122	29.050783382580086
33	32.15203078508486	21.691096803467467	18.97292232465921	27.18395008678846
34	30.36522991824759	20.519271876747553	21.23469585390567	27.880802351099188
35	32.681103333806846	20.885410466568928	20.195786271595146	26.23769992802908
36	35.83316111351801	19.83121130493646	19.229599917511504	25.106027664034023
37	35.20704067057602	19.734805086259634	19.85704058944463	25.20111365371972
38	31.63785690989415	21.911221017101465	23.423333122391718	23.027588950612667
39	31.210080590022876	23.4857969175087	22.918268089681753	22.38585440278667
40	31.200499710360933	25.52858944816936	19.28482089212962	23.986089949340084
41	31.93411924573769	24.48407442433222	18.536199425088213	25.045606904841875
42	32.91929772078322	23.784832471791724	18.104919704262805	25.19095010316225
43	32.8574608500475	21.97220969602723	18.676305960929778	26.494023492995485
44	31.028154510160377	22.174425999099885	19.666662093806476	27.130757396933262
45	28.65890401522202	22.967227196068166	20.167065759352198	28.206803029357612
46	28.67029928779915	21.259514683822857	20.689905942166302	29.38028008621169
47	28.381228143387784	19.2167221531622	22.014715169077082	30.387334534372933
48	29.955870424023622	18.412813331274073	20.902920095758642	30.72839614894366
49	30.59629211843944	19.414350844880175	21.51073434676766	28.47862268991273
50	29.525859303122658	21.444877784410885	20.605824319267068	28.42343859319939
51	28.074167957024553	21.77784101109184	21.40562494256266	28.742366089320942
52	29.177953149424695	21.265422524168674	22.31912014629612	27.237504180110506
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	6.5
4	11.0
5	35.5
6	60.0
7	352.0
8	644.0
9	1373.5
10	2103.0
11	3266.0
12	4429.0
13	5657.0
14	7869.5
15	8854.0
16	10726.5
17	12599.0
18	16363.5
19	20128.0
20	26576.0
21	33024.0
22	40710.5
23	48397.0
24	57299.5
25	66202.0
26	68955.5
27	71709.0
28	75194.0
29	78679.0
30	80974.0
31	83269.0
32	91898.0
33	100527.0
34	112541.0
35	124555.0
36	135004.5
37	145454.0
38	164037.5
39	213269.0
40	243917.0
41	286492.5
42	329068.0
43	377040.0
44	425012.0
45	475492.0
46	525972.0
47	562900.0
48	599828.0
49	656538.0
50	713248.0
51	799524.5
52	885801.0
53	996453.0
54	1107105.0
55	1219292.5
56	1331480.0
57	1356665.0
58	1381850.0
59	1315418.5
60	1248987.0
61	1156684.5
62	1064382.0
63	935719.0
64	704866.5
65	602677.0
66	551752.0
67	500827.0
68	420856.0
69	340885.0
70	284805.5
71	228726.0
72	174776.0
73	120826.0
74	92636.0
75	64446.0
76	46898.5
77	29351.0
78	20626.5
79	11902.0
80	7636.0
81	3370.0
82	2295.0
83	1220.0
84	673.5
85	127.0
86	77.0
87	27.0
88	19.5
89	6.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1.3558254E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.998145863267606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.88290814490888	5.100452221372069
2	8.453259913718657	1.1831429179263222
3	4.080496441522302	0.8566772787695246
4	2.4991152196159336	0.6995669134393744
5	1.661230183265144	0.5812765567476127
6	1.1003770739975063	0.46203595610580966
7	0.7485984363627936	0.3667160735476617
8	0.5357245658586004	0.2999262923531359
9	0.37513614256122035	0.23627316998042738
>10	2.5582585590356293	3.7679284457285585
>50	0.959519223791029	5.037219175253038
>100	3.740314214389115	61.04801387208983
>500	0.36533940680528604	16.715308327032616
>1k	0.0397224741680732	3.645462799654192
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0022274254487340333	0.0	0.0	0.0	0.0
12	0.00543580316462577	0.0	0.0	0.0	0.0
13	0.013475186406745294	0.0	0.0	0.0	0.0
14	0.038264514000106505	0.0	0.0	0.0	0.0
15	0.06152709633556061	0.0	0.0	0.0	0.0
16	0.07832866975349481	0.0	0.0	0.0	0.0
17	0.09170059802685508	0.0	0.0	0.0	0.0
18	0.10718194245365222	0.0	0.0	0.0	0.0
19	0.12034735446024244	0.0	0.0	0.0	0.0
20	0.13450846989590254	0.0	0.0	0.0	0.0
21	0.14838193767427577	0.0	0.0	0.0	0.0
22	0.16443857741564658	0.0	0.0	0.0	0.0
23	0.18228748332934314	0.0	0.0	0.0	0.0
24	0.20267358909192879	0.0	0.0	0.0	0.0
25	0.22242539489229218	0.0	0.0	0.0	0.0
26	0.24520118888464548	0.0	0.0	0.0	0.0
27	0.26942259674438906	0.0	0.0	0.0	0.0
28	0.2944036894426082	0.0	0.0	0.0	0.0
29	0.320269851855556	0.0	0.0	0.0	0.0
30	0.34852570249827153	0.0	0.0	0.0	0.0
31	0.3749376579019688	0.0	0.0	0.0	0.0
32	0.39883454019964515	0.0	0.0	0.0	0.0
33	0.41868960413339357	0.0	0.0	0.0	0.0
34	0.4368114065424648	0.0	0.0	0.0	0.0
35	0.45376786716047657	0.0	0.0	0.0	0.0
36	0.46933771855874656	0.0	0.0	0.0	0.0
37	0.4847010536902465	0.0	0.0	0.0	0.0
38	0.502033668937018	0.0	0.0	0.0	0.0
39	0.5252593733676917	0.0	0.0	0.0	0.0
40	0.5581470888508211	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCTA	40	5.6188583E-9	46.000004	23
ATGGGGC	235330	0.0	43.973953	9
CAACTTA	74070	0.0	43.754963	3
ATATCGC	100	0.0	43.699997	42
AACTTAT	312735	0.0	43.626713	4
TATGGGC	119635	0.0	43.61993	8
CACTTAT	331585	0.0	43.61666	4
TATGGGG	937415	0.0	43.592327	8
ATGGGAC	43520	0.0	43.58479	9
TCACTTA	118930	0.0	43.482048	3
ACACTTA	79645	0.0	43.429844	3
TTATGGG	1406665	0.0	43.39958	7
ACTTATG	1433085	0.0	43.396324	5
CTTATGG	1428475	0.0	43.390175	6
GCACTTA	85190	0.0	43.32175	3
TAACTTA	112635	0.0	43.284145	3
ATGGGAG	110325	0.0	43.246044	9
GACTTAT	304880	0.0	43.23891	4
ATGGGCT	50995	0.0	43.221687	9
AAACTTA	72065	0.0	43.216957	3
>>END_MODULE