FastQCFastQC Report
Fri 17 Jun 2016
SRR1527971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13441530
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGC2321150.043.934999
CAACTTA730500.043.5724833
CACTTAT3278000.043.5372164
AACTTAT3109300.043.5337874
TATGGGG9282350.043.506328
TATGGGC1185350.043.4794778
ATGGGAC440600.043.4264649
ACACTTA794650.043.415343
TCACTTA1179650.043.393213
ATGGGAG1091700.043.3833479
ACTTATG14223050.043.3494225
CTTATGG14180350.043.3388446
TTATGGG13965400.043.337747
AAACTTA711450.043.3264433
GCACTTA840700.043.2723923
TAACTTA1124300.043.2321433
ATGGGGG3780350.043.1544729
GACTTAT3043000.043.1195224
TACACTT283800.043.114872
TACTTAT4849250.043.1039244
Produced by FastQC (version 0.11.5)