##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527971_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13441530
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.897034042999568	33.0	31.0	34.0	30.0	34.0
2	32.26892429656446	34.0	31.0	34.0	30.0	34.0
3	32.475966128855866	34.0	31.0	34.0	30.0	34.0
4	35.992036174453354	37.0	35.0	37.0	35.0	37.0
5	35.951278463091626	37.0	35.0	37.0	35.0	37.0
6	36.11504560864723	37.0	36.0	37.0	35.0	37.0
7	36.31166511550396	37.0	37.0	37.0	35.0	37.0
8	36.21271603753442	37.0	37.0	37.0	35.0	37.0
9	37.674182700927645	39.0	38.0	39.0	35.0	39.0
10	38.041519975776566	39.0	39.0	39.0	35.0	39.0
11	38.19321795956264	39.0	39.0	39.0	37.0	39.0
12	38.09555251522706	39.0	39.0	39.0	37.0	39.0
13	38.12093764623521	39.0	39.0	39.0	37.0	39.0
14	38.8356958620038	40.0	39.0	40.0	36.0	40.0
15	38.27772411325199	40.0	38.0	40.0	35.0	40.0
16	37.5317513705657	40.0	36.0	40.0	33.0	40.0
17	37.463605408015304	40.0	36.0	40.0	33.0	40.0
18	37.489813659605716	40.0	36.0	40.0	33.0	40.0
19	37.616505487098564	40.0	37.0	40.0	33.0	40.0
20	37.61916493137314	40.0	36.0	40.0	33.0	40.0
21	37.57907708423074	40.0	36.0	40.0	33.0	40.0
22	37.54577313743302	40.0	36.0	40.0	33.0	40.0
23	37.473102987531924	40.0	36.0	40.0	33.0	40.0
24	37.4495602063158	40.0	36.0	40.0	33.0	40.0
25	37.40720758723151	40.0	36.0	40.0	33.0	40.0
26	37.39025743349158	40.0	36.0	40.0	33.0	40.0
27	37.42268670307621	40.0	36.0	40.0	33.0	40.0
28	37.24617108320258	40.0	35.0	40.0	33.0	40.0
29	37.33753739343661	40.0	35.0	40.0	33.0	40.0
30	37.353662120309224	40.0	35.0	40.0	33.0	40.0
31	37.344960655520616	40.0	35.0	40.0	33.0	40.0
32	37.27961184478255	40.0	35.0	40.0	33.0	40.0
33	37.20233954021603	40.0	35.0	40.0	33.0	40.0
34	37.11910370322426	40.0	35.0	40.0	33.0	40.0
35	36.9519387301892	40.0	35.0	40.0	32.0	40.0
36	36.50158545939339	39.0	35.0	40.0	31.0	40.0
37	36.5838670151389	39.0	35.0	40.0	31.0	40.0
38	36.46443239720478	39.0	35.0	40.0	31.0	40.0
39	36.372320487325474	39.0	35.0	40.0	31.0	40.0
40	36.27963885063679	39.0	35.0	40.0	31.0	40.0
41	36.16001720042287	38.0	35.0	40.0	31.0	40.0
42	35.855240065677044	38.0	35.0	40.0	30.0	40.0
43	35.68650019752216	38.0	35.0	40.0	30.0	40.0
44	35.52520196733556	37.0	35.0	40.0	30.0	40.0
45	35.122353854062744	37.0	34.0	40.0	28.0	40.0
46	35.143595483549866	37.0	34.0	40.0	28.0	40.0
47	35.05739309438732	36.0	34.0	40.0	28.0	40.0
48	34.91321121925852	36.0	34.0	40.0	28.0	40.0
49	34.75902624180432	36.0	34.0	40.0	28.0	40.0
50	34.54136753777286	35.0	34.0	39.0	27.0	40.0
51	34.455354263986315	35.0	34.0	39.0	27.0	40.0
52	33.948542539428175	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	9.0
13	17.0
14	31.0
15	73.0
16	225.0
17	632.0
18	1625.0
19	3474.0
20	6678.0
21	12070.0
22	19754.0
23	30854.0
24	45259.0
25	64455.0
26	88737.0
27	119245.0
28	157218.0
29	201919.0
30	255211.0
31	312026.0
32	378937.0
33	481377.0
34	737003.0
35	1484753.0
36	1123732.0
37	2112776.0
38	3818791.0
39	1984649.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.555360141293438	20.470139931986907	34.953401882077415	17.021098044642237
2	21.448748765951496	22.169857151678418	36.69614247782805	19.685251604542042
3	21.89370555286489	21.31752114528629	37.340600363202704	19.44817293864612
4	21.144683678122952	21.961517773646303	33.77006189027588	23.12373665795486
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.70071859379104	0.2266185471445587	0.05837877086909005	0.01428408819531705
12	99.62391186122413	0.268511099554887	0.06046930669350885	0.04710773252747269
13	98.4489042542032	0.8551853844019245	0.21138962603215555	0.48452073536271545
14	66.41165849423392	19.489671190705224	5.451529699372021	8.647140615688839
15	37.85699247035122	24.45775890095845	15.18451396530008	22.500734663390254
16	31.804816862366113	17.034995272115598	24.966919688458084	26.193268177060204
17	24.43875808780697	16.608399490236604	28.026921042470608	30.925921379485814
18	23.357958506211716	17.63592388664088	31.59841922757305	27.407698379574345
19	26.450158575697856	17.369912502520172	28.194870673204615	27.985058248577353
20	30.278502521662343	17.29615601795331	24.56991131217949	27.855430148204857
21	31.694874021037784	18.348662689440857	21.35967408472101	28.59678920480035
22	31.63341524365158	19.53941255199371	21.836375769722643	26.99079643463207
23	29.99772347344387	21.020538584521255	20.782656438664347	28.199081503370525
24	30.31213708558475	23.019849674851002	19.641923203682914	27.026090035881335
25	31.119366619722605	20.93553338050058	22.39459347261807	25.55050652715874
26	30.51081982482649	20.074396292683943	21.899248076669846	27.515535805819724
27	32.131446345765696	20.095204935747642	20.97325974052061	26.800088977966052
28	33.45064140763738	19.966774615687353	19.56201414571109	27.02056983096418
29	31.651381948334752	18.71784685225566	19.009748146230375	30.621023053179215
30	29.691597608307983	18.205658135643784	20.785855479249758	31.316888776798475
31	27.99156048455793	18.462816360935104	20.815480082996505	32.73014307151046
32	29.118463448729422	22.25315124096736	19.57873843230644	29.049646877996775
33	32.12703464560954	21.72805476757482	18.956004264395496	27.18890632242014
34	30.337320230658264	20.523310962368125	21.232143959802194	27.907224847171413
35	32.693220191451424	20.92627104206143	20.189353444139172	26.191155322347974
36	35.88855584148531	19.802329050338763	19.2960697182538	25.013045389922127
37	35.19603051140756	19.751300633186847	19.83425249952944	25.218416355876155
38	31.61149065619762	21.917646279850583	23.42772734949072	23.043135714461076
39	31.17609379289411	23.53188215924824	22.946167586576827	22.34585646128082
40	31.198286207001736	25.547783622846502	19.271496622780294	23.982433547371468
41	31.926841661626316	24.474081447573305	18.53811284876052	25.060964042039856
42	32.97850765500653	23.797238855993328	18.149727002804	25.07452648619614
43	32.81283455082866	22.020551231891012	18.701137444918846	26.46547677236148
44	31.27596337619304	22.094396992009095	19.595693347409114	27.033946284388755
45	28.668953608703774	23.044259098480605	20.200974145056403	28.085813147759218
46	28.711813312918988	21.326485898554704	20.68605285261425	29.27564793591206
47	28.372774527899725	19.20155666802812	21.990718318524753	30.4349504855474
48	29.973105740194754	18.425075121656537	20.961594401827767	30.640224736320942
49	30.657053177726045	19.37946052272323	21.512610543591393	28.45087575595933
50	29.648447758551296	21.41221274661441	20.655728923716275	28.283610571118018
51	28.08683237696899	21.8110587113223	21.380430650379832	28.721678261328883
52	29.200344008457368	21.231995167216827	22.302029605260714	27.26563121906509
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	5.5
4	8.0
5	32.5
6	57.0
7	367.0
8	677.0
9	1402.0
10	2127.0
11	3178.5
12	4230.0
13	5526.5
14	7809.5
15	8796.0
16	10700.5
17	12605.0
18	16142.0
19	19679.0
20	26146.0
21	32613.0
22	40172.5
23	47732.0
24	56535.0
25	65338.0
26	68058.0
27	70778.0
28	74807.0
29	78836.0
30	80870.5
31	82905.0
32	91363.0
33	99821.0
34	112192.5
35	124564.0
36	134753.0
37	144942.0
38	162847.5
39	211407.5
40	242062.0
41	283812.5
42	325563.0
43	373798.5
44	422034.0
45	471504.5
46	520975.0
47	558852.0
48	596729.0
49	653393.0
50	710057.0
51	796223.0
52	882389.0
53	990691.5
54	1098994.0
55	1208201.5
56	1317409.0
57	1342490.5
58	1367572.0
59	1302132.0
60	1236692.0
61	1145402.5
62	1054113.0
63	926671.0
64	698087.5
65	596946.0
66	545143.5
67	493341.0
68	415765.5
69	338190.0
70	282801.0
71	227412.0
72	173222.0
73	119032.0
74	91540.0
75	64048.0
76	46618.5
77	29189.0
78	20394.5
79	11600.0
80	7471.0
81	3342.0
82	2263.5
83	1185.0
84	646.0
85	107.0
86	67.0
87	27.0
88	16.5
89	3.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1.344153E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.319956753573381
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.59885407232237	5.314204721683845
2	8.666422636830028	1.2687567781957036
3	4.126995506441638	0.906282858880334
4	2.596503013867197	0.7602515908812332
5	1.673074726635735	0.6123417322235093
6	1.0878607648536391	0.47778562515827405
7	0.6893665328089206	0.35322932452654876
8	0.5395978257809176	0.3159866199230833
9	0.3801708306926692	0.2504550635676366
>10	2.598136796377116	4.000910335016469
>50	1.0018187051898453	5.52401957191263
>100	3.6821232630805683	61.58484592394138
>500	0.32604338862220955	15.471181208126152
>1k	0.03303193649713376	3.1597486459633615
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0019863810146612776	0.0	0.0	0.0	0.0
12	0.0052895764098283456	0.0	0.0	0.0	0.0
13	0.01312350602944754	0.0	0.0	0.0	0.0
14	0.03792723001027413	0.0	0.0	0.0	0.0
15	0.062277136605728665	0.0	0.0	0.0	0.0
16	0.07967842946450293	0.0	0.0	0.0	0.0
17	0.09366493248908421	0.0	0.0	0.0	0.0
18	0.10891617248929251	0.0	0.0	0.0	0.0
19	0.1226199696016748	0.0	0.0	0.0	0.0
20	0.1373801940701691	0.0	0.0	0.0	0.0
21	0.15176843707524368	0.0	0.0	0.0	0.0
22	0.16740653779740847	0.0	0.0	0.0	0.0
23	0.1862957565098616	0.0	0.0	0.0	0.0
24	0.20630091961257388	0.0	0.0	0.0	0.0
25	0.22722859674456702	0.0	0.0	0.0	0.0
26	0.24939125233511364	0.0	0.0	0.0	0.0
27	0.2726698523159194	0.0	0.0	0.0	0.0
28	0.29599309007233554	0.0	0.0	0.0	0.0
29	0.32050666851169474	0.0	0.0	0.0	0.0
30	0.34861358788768837	0.0	0.0	0.0	0.0
31	0.37481596217097307	0.0	0.0	0.0	0.0
32	0.39812432066885245	0.0	0.0	0.0	0.0
33	0.4172144093715522	0.0	0.0	0.0	0.0
34	0.4341470055864176	0.0	0.0	0.0	0.0
35	0.4497255892744353	0.0	0.0	0.0	0.0
36	0.4647313215087866	0.0	0.0	0.0	0.0
37	0.47952874412362284	0.0	0.0	0.0	0.0
38	0.49703419179215463	0.0	0.0	0.0	0.0
39	0.5197845780949044	0.0	0.0	0.0	0.0
40	0.5505176866026412	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGGC	232115	0.0	43.93499	9
CAACTTA	73050	0.0	43.572483	3
CACTTAT	327800	0.0	43.537216	4
AACTTAT	310930	0.0	43.533787	4
TATGGGG	928235	0.0	43.50632	8
TATGGGC	118535	0.0	43.479477	8
ATGGGAC	44060	0.0	43.426464	9
ACACTTA	79465	0.0	43.41534	3
TCACTTA	117965	0.0	43.39321	3
ATGGGAG	109170	0.0	43.383347	9
ACTTATG	1422305	0.0	43.349422	5
CTTATGG	1418035	0.0	43.338844	6
TTATGGG	1396540	0.0	43.33774	7
AAACTTA	71145	0.0	43.326443	3
GCACTTA	84070	0.0	43.272392	3
TAACTTA	112430	0.0	43.232143	3
ATGGGGG	378035	0.0	43.154472	9
GACTTAT	304300	0.0	43.119522	4
TACACTT	28380	0.0	43.11487	2
TACTTAT	484925	0.0	43.103924	4
>>END_MODULE