FastQCFastQC Report
Fri 17 Jun 2016
SRR1527970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13720150
Sequences flagged as poor quality0
Sequence length59
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGC2362300.050.5242169
AACTTAT3208750.050.1648034
TATGGGG9481050.050.1048858
CACTTAT3360850.050.092054
CAACTTA756150.050.0245973
ACACTTA814750.049.939373
TATGGGC1215150.049.918538
TTATGGG14260850.049.88827
ACTTATG14538650.049.8641785
CTTATGG14489300.049.8580746
ATGGGAC437500.049.844239
AAACTTA731250.049.8363083
TCACTTA1208200.049.73633
TAACTTA1174800.049.7314873
GACTTAT3095050.049.683914
ATGGGAG1101900.049.664359
GCACTTA864700.049.631953
ATGGGGG3893050.049.6039859
TACTTAT4934150.049.4665954
CTACTTA1016500.049.4440733
Produced by FastQC (version 0.11.5)