##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527970_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13720150
Sequences flagged as poor quality	0
Sequence length	59
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.525511310007545	31.0	31.0	34.0	30.0	34.0
2	32.004137418322685	33.0	31.0	34.0	30.0	34.0
3	32.20242628542691	34.0	31.0	34.0	30.0	34.0
4	35.80544432823256	37.0	35.0	37.0	35.0	37.0
5	35.59047342776865	37.0	35.0	37.0	33.0	37.0
6	35.864151412338785	37.0	35.0	37.0	35.0	37.0
7	36.072067725207084	37.0	35.0	37.0	35.0	37.0
8	36.013217056664836	37.0	35.0	37.0	35.0	37.0
9	37.36726515380663	39.0	37.0	39.0	34.0	39.0
10	37.77437149010762	39.0	37.0	39.0	35.0	39.0
11	37.93991486973539	39.0	37.0	39.0	35.0	39.0
12	37.867538547319086	39.0	37.0	39.0	35.0	39.0
13	37.86082761485844	39.0	38.0	39.0	35.0	39.0
14	38.330301417987414	40.0	38.0	40.0	35.0	40.0
15	37.759999781343495	40.0	38.0	40.0	33.0	40.0
16	37.03447178055634	39.0	35.0	40.0	32.0	40.0
17	36.87847035200053	39.0	35.0	40.0	32.0	40.0
18	36.84967671636243	39.0	35.0	40.0	31.0	40.0
19	36.95302026581342	39.0	35.0	40.0	32.0	40.0
20	37.048455301144664	39.0	35.0	40.0	32.0	40.0
21	36.97167472658827	39.0	35.0	40.0	31.0	40.0
22	36.94793570041144	39.0	35.0	40.0	31.0	40.0
23	36.89871444554178	39.0	35.0	40.0	31.0	40.0
24	36.86377510449959	39.0	35.0	40.0	31.0	40.0
25	36.81350356956739	39.0	35.0	40.0	31.0	40.0
26	36.66721872574279	39.0	35.0	40.0	31.0	40.0
27	36.57989438890974	39.0	35.0	40.0	31.0	40.0
28	36.5670655933062	39.0	35.0	40.0	31.0	40.0
29	36.51706344318393	39.0	35.0	40.0	31.0	40.0
30	36.47700579075302	39.0	35.0	40.0	30.0	40.0
31	36.46654504506146	39.0	35.0	40.0	31.0	40.0
32	36.3378679533387	39.0	35.0	40.0	30.0	40.0
33	36.18664263874666	38.0	35.0	40.0	30.0	40.0
34	36.08076092462546	38.0	35.0	40.0	30.0	40.0
35	35.93003728093352	38.0	35.0	40.0	30.0	40.0
36	35.74874830085677	38.0	35.0	40.0	29.0	40.0
37	35.59416391220213	38.0	34.0	40.0	29.0	40.0
38	35.32219086526022	38.0	34.0	40.0	27.0	40.0
39	35.16369893915154	38.0	34.0	40.0	27.0	40.0
40	35.03370225544182	37.0	34.0	40.0	27.0	40.0
41	34.90746128868854	37.0	34.0	40.0	26.0	40.0
42	34.78282190792375	37.0	34.0	40.0	26.0	40.0
43	34.56682397787196	37.0	33.0	40.0	26.0	40.0
44	34.40290798569986	36.0	33.0	40.0	25.0	40.0
45	34.177987704216065	36.0	33.0	40.0	24.0	40.0
46	34.00262110836981	35.0	33.0	39.0	24.0	40.0
47	33.85575339919753	35.0	33.0	39.0	24.0	40.0
48	33.67558904239385	35.0	33.0	39.0	23.0	40.0
49	33.550626268663244	35.0	33.0	39.0	24.0	40.0
50	33.346120268364416	35.0	33.0	39.0	23.0	40.0
51	33.15754536211339	35.0	32.0	38.0	23.0	40.0
52	32.9710108854495	35.0	32.0	38.0	23.0	40.0
53	32.762723658269046	35.0	32.0	38.0	23.0	40.0
54	32.59818726471649	35.0	31.0	37.0	22.0	40.0
55	32.46333691687044	35.0	31.0	37.0	22.0	40.0
56	32.25583612424062	35.0	31.0	37.0	21.0	39.0
57	32.094767622802955	35.0	31.0	36.0	21.0	39.0
58	31.910542668994143	35.0	31.0	36.0	20.0	39.0
59	31.37916699161452	34.0	30.0	35.0	20.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	20.0
14	77.0
15	270.0
16	1138.0
17	3290.0
18	7667.0
19	14811.0
20	25094.0
21	39854.0
22	58118.0
23	81050.0
24	108254.0
25	142535.0
26	180581.0
27	225224.0
28	270994.0
29	313896.0
30	367973.0
31	444384.0
32	547114.0
33	700535.0
34	1035538.0
35	1542225.0
36	1542758.0
37	2373889.0
38	3022485.0
39	670369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.61205963491653	20.76500621348892	34.46187541681396	17.161058734780596
2	21.00077623058057	22.619913047597876	36.60524119634261	19.77406952547895
3	21.89991363068188	21.513430975608866	37.06994457057685	19.5167108231324
4	21.219556637500318	22.127542337365117	33.497629399095494	23.155271626039074
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.69641002467175	0.23591578809269578	0.05064084576334807	0.017033341472214227
12	99.62308721114566	0.2723439612540679	0.05110731296669497	0.053461514633586366
13	98.42764838576838	0.8723811328593347	0.2039263419131715	0.49604413945911674
14	66.17674005021811	19.641731322179425	5.504728446846427	8.676800180756041
15	37.84985586892272	24.593309839907	15.192377634355308	22.364456656814976
16	31.90944705415028	17.10371971151919	24.876236775837	26.11059645849353
17	24.45659121802604	16.680429878682084	27.900635197137056	30.962343706154815
18	23.28653112393086	17.753552257081736	31.55445822385324	27.405458395134165
19	26.49352958969107	17.452928721624765	28.213022452378432	27.840519236305727
20	30.382838380046866	17.380575285255627	24.54292409339548	27.693662241302025
21	31.677693028137448	18.459397309796174	21.452075961268648	28.41083370079773
22	31.604341060411144	19.69195672058979	21.87323753749048	26.830464681508587
23	29.958229319650297	21.20113847151817	20.81047218871514	28.030160020116394
24	30.29372127855745	23.20612383975394	19.62359012109926	26.87656476058935
25	31.024580635051368	21.09847195548154	22.454251593459254	25.42269581600784
26	30.41499546287759	20.16909436121325	22.036807177764093	27.379102998145065
27	32.094845901830524	20.203700396861553	21.051387922143707	26.650065779164223
28	33.380043221101815	20.032936957686324	19.58183401784966	27.00518580336221
29	31.6051428009169	18.809225846656197	19.040513405465685	30.54511794696122
30	29.579705761234386	18.314165661454137	20.89236633710273	31.21376224020874
31	27.805133325801833	18.60127622511416	20.902286053723902	32.69130439536011
32	28.972095786124786	22.474076449601498	19.57806583747262	28.97576192680109
33	32.11770279479452	21.899170198576545	18.972321731176407	27.010805275452526
34	30.314763322558424	20.655160475650778	21.301443497337857	27.728632704452938
35	32.647041030892524	20.991745717065776	20.223911546156565	26.13730170588514
36	35.86309187581768	19.896998210660964	19.307974038184714	24.931935875336638
37	35.20320113118297	19.861481106256125	19.849761117772037	25.08555664478887
38	31.512344981651076	22.070983188959303	23.548612806711297	22.868059022678324
39	31.031490180500942	23.73949264403086	22.968269297347334	22.260747878120863
40	31.045892355404277	25.876867235416523	19.25312041049114	23.82411999868806
41	31.84726843365415	24.73818434929647	18.49389401719369	24.920653199855686
42	32.817155789113094	24.032645415684232	18.092703068115146	25.05749572708753
43	32.775807844666424	22.1690870726632	18.68897934789343	26.36612573477695
44	30.96315273521062	22.263291582089117	19.65840023614902	27.115155446551242
45	28.472844684642663	23.22833205176328	20.13143442309304	28.16738884050102
46	28.553142640568797	21.450042455804056	20.675160256994275	29.321654646632872
47	28.256433056489904	19.355604712776465	21.967179659114514	30.420782571619114
48	29.85752342357773	18.531320721712227	20.88951651403228	30.721639340677765
49	30.5639296946462	19.576323874010125	21.451850016217026	28.407896415126654
50	29.427586433092934	21.65864075830075	20.550154335047356	28.36361847355896
51	27.924665546659476	21.972675225853944	21.412630328385625	28.690028899100955
52	29.043939023990262	21.432863343330794	22.38463136335973	27.138566269319213
53	29.718778584782235	21.12693374343575	21.719048261134173	27.435239410647842
54	28.104167957347403	20.67416172563711	22.372401176371977	28.849269140643507
55	27.167654872577923	21.461441748085843	22.9294359026687	28.44146747666753
56	26.949741803114396	21.236626421722796	23.377812924785808	28.435818850376997
57	27.034573237173063	20.321716599308317	24.68693126532873	27.95677889818989
58	27.332142870158126	20.32346585132087	24.290762127236217	28.053629151284788
59	27.120169968987216	20.017703888077026	22.782965200817777	30.079160942117976
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.0
4	20.0
5	36.0
6	256.5
7	477.0
8	970.5
9	2115.5
10	2767.0
11	3536.5
12	4306.0
13	4306.0
14	4725.5
15	5145.0
16	5903.5
17	6662.0
18	8640.5
19	13776.0
20	16933.0
21	22637.5
22	28342.0
23	34781.5
24	41221.0
25	48226.5
26	62328.0
27	69424.0
28	74744.0
29	80064.0
30	86719.0
31	98262.0
32	103150.0
33	112304.5
34	121459.0
35	131610.5
36	146027.5
37	150293.0
38	170787.0
39	191281.0
40	217230.5
41	243180.0
42	263829.5
43	311508.5
44	338538.0
45	362642.5
46	386747.0
47	427612.5
48	518005.0
49	567532.0
50	616969.5
51	666407.0
52	776057.5
53	985528.5
54	1085349.0
55	1172556.0
56	1259763.0
57	1252235.0
58	1158383.0
59	1072059.0
60	1017453.0
61	962847.0
62	886367.5
63	809888.0
64	711551.0
65	555353.5
66	497493.0
67	459213.0
68	420933.0
69	376861.0
70	271456.5
71	210124.0
72	163266.0
73	116408.0
74	89706.0
75	49014.5
76	35025.0
77	26348.5
78	17672.0
79	11698.5
80	5725.0
81	4003.0
82	1578.0
83	875.0
84	613.5
85	352.0
86	282.0
87	126.0
88	40.0
89	20.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1.372015E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.369348162788143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.93785431052645	9.0884129704261
2	7.300966783891086	1.6601446658201873
3	3.262224771173027	1.1126810762621409
4	1.8789517132029545	0.8544982483388659
5	1.1730036782091824	0.666814360689565
6	0.6952342402968569	0.47426160795758876
7	0.48491575842861445	0.385922326103817
8	0.3388280026336933	0.30818028233956457
9	0.25194717146036805	0.25780275998663443
>10	1.5938094443324053	3.7729432791480604
>50	0.6024634994391577	5.15523442358429
>100	2.25916275713978	58.5646189507136
>500	0.20137533994645027	14.857712090302048
>1k	0.0192625293201104	2.8407729583276424
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.001997062714328925	0.0	0.0	0.0	0.0
12	0.0051967361872865825	0.0	0.0	0.0	0.0
13	0.013039216043556375	0.0	0.0	0.0	0.0
14	0.03781299767130826	0.0	0.0	0.0	0.0
15	0.05947456842673003	0.0	0.0	0.0	0.0
16	0.07606330834575424	0.0	0.0	0.0	0.0
17	0.0891171014894152	0.0	0.0	0.0	0.0
18	0.10350469929264622	0.0	0.0	0.0	0.0
19	0.11596811988207126	0.0	0.0	0.0	0.0
20	0.12987467338185077	0.0	0.0	0.0	0.0
21	0.1433001825781788	0.0	0.0	0.0	0.0
22	0.15802305368381542	0.0	0.0	0.0	0.0
23	0.17445144550168912	0.0	0.0	0.0	0.0
24	0.19351100388844145	0.0	0.0	0.0	0.0
25	0.21327755163026643	0.0	0.0	0.0	0.0
26	0.23411551622977883	0.0	0.0	0.0	0.0
27	0.25523044573127845	0.0	0.0	0.0	0.0
28	0.2781237814455381	0.0	0.0	0.0	0.0
29	0.3016730866645044	0.0	0.0	0.0	0.0
30	0.328932263860089	0.0	0.0	0.0	0.0
31	0.3543109951421814	0.0	0.0	0.0	7.2885500522953465E-6
32	0.37636614760042714	0.0	0.0	0.0	7.2885500522953465E-6
33	0.39554232278801615	0.0	0.0	0.0	7.2885500522953465E-6
34	0.41142407335196773	0.0	0.0	0.0	7.2885500522953465E-6
35	0.42686851091278155	0.0	0.0	0.0	7.2885500522953465E-6
36	0.44147476521758144	0.0	0.0	0.0	7.2885500522953465E-6
37	0.45570201491966195	0.0	0.0	0.0	7.2885500522953465E-6
38	0.47258229684077796	0.0	0.0	0.0	7.2885500522953465E-6
39	0.49390130574374186	0.0	0.0	0.0	7.2885500522953465E-6
40	0.5258688862731092	0.0	0.0	0.0	7.2885500522953465E-6
41	0.5522534374624184	0.0	0.0	0.0	7.2885500522953465E-6
42	0.5722094875056031	0.0	0.0	0.0	7.2885500522953465E-6
43	0.5905329023370736	0.0	0.0	0.0	7.2885500522953465E-6
44	0.6080327110126347	0.0	0.0	0.0	7.2885500522953465E-6
45	0.6263925685943666	0.0	0.0	0.0	1.4577100104590693E-5
46	0.6447159834258371	0.0	0.0	0.0	1.4577100104590693E-5
47	0.6610350469929265	0.0	0.0	0.0	1.4577100104590693E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGGC	236230	0.0	50.524216	9
AACTTAT	320875	0.0	50.164803	4
TATGGGG	948105	0.0	50.104885	8
CACTTAT	336085	0.0	50.09205	4
CAACTTA	75615	0.0	50.024597	3
ACACTTA	81475	0.0	49.93937	3
TATGGGC	121515	0.0	49.91853	8
TTATGGG	1426085	0.0	49.8882	7
ACTTATG	1453865	0.0	49.864178	5
CTTATGG	1448930	0.0	49.858074	6
ATGGGAC	43750	0.0	49.84423	9
AAACTTA	73125	0.0	49.836308	3
TCACTTA	120820	0.0	49.7363	3
TAACTTA	117480	0.0	49.731487	3
GACTTAT	309505	0.0	49.68391	4
ATGGGAG	110190	0.0	49.66435	9
GCACTTA	86470	0.0	49.63195	3
ATGGGGG	389305	0.0	49.603985	9
TACTTAT	493415	0.0	49.466595	4
CTACTTA	101650	0.0	49.444073	3
>>END_MODULE