FastQCFastQC Report
Fri 17 Jun 2016
SRR1527969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964004
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACCTCGCAGGGGGGCGTAACCCGAGGCCACCGAAAACCCGAGATGCAGAC26800.13645593389830163No Hit
TGATCTCGCAGGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATC24670.1256107421369814No Hit
CCTACTCGCAGGGGGACTGTAGCGGTGAAGGGCCGGAGGGGGCTCTGCGTTC22970.11695495528522344No Hit
ATGCCTCGCAGGGGGTAACCGCTGGAGGCTTCTGCTGACGGCGTCGGACCTG22880.11649670774601273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCT253.4180463E-546.040
AACGTTA501.6370905E-1146.032
GTCATCG501.6370905E-1146.012
AACCGTG301.8620831E-646.023
CTAGACG301.8620831E-646.046
TCTTGTA253.4180463E-546.024
ATTAGTA501.6370905E-1146.024
GGTAATA301.8620831E-646.013
ATTAGCC800.046.017
GGTCGTA950.046.042
GTATTAG253.4180463E-546.038
CGTATAG206.312554E-446.036
GATCGAT206.312554E-446.014
CCTAGTA600.046.031
GCGAATA206.312554E-446.016
ACGTTAG501.6370905E-1146.033
CTATCCT206.312554E-446.031
TTAGCAC405.6152203E-946.019
GCAACTA206.312554E-446.013
CGGTTTA253.4180463E-546.019
Produced by FastQC (version 0.11.5)