##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527969_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1964004
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.937809698961917	33.0	31.0	34.0	30.0	34.0
2	32.378629065928585	34.0	31.0	34.0	30.0	34.0
3	32.40442687489435	34.0	31.0	34.0	30.0	34.0
4	36.057806908743565	37.0	35.0	37.0	35.0	37.0
5	36.08952120260447	37.0	35.0	37.0	35.0	37.0
6	36.10695242983212	37.0	36.0	37.0	35.0	37.0
7	36.03348771183765	37.0	35.0	37.0	35.0	37.0
8	35.836271718387536	37.0	35.0	37.0	33.0	37.0
9	37.33368465644673	39.0	37.0	39.0	34.0	39.0
10	37.34558840002362	39.0	37.0	39.0	34.0	39.0
11	37.89560102728915	39.0	37.0	39.0	35.0	39.0
12	38.02387418762895	39.0	38.0	39.0	37.0	39.0
13	38.069632750238796	39.0	39.0	39.0	37.0	39.0
14	38.739765295793696	40.0	39.0	40.0	36.0	40.0
15	38.07076309416885	40.0	38.0	40.0	34.0	40.0
16	37.40781637919271	39.0	36.0	40.0	33.0	40.0
17	37.33270960751607	40.0	36.0	40.0	33.0	40.0
18	37.3506769843646	40.0	36.0	40.0	33.0	40.0
19	37.44693035248401	40.0	37.0	40.0	33.0	40.0
20	37.521380302687774	40.0	37.0	40.0	33.0	40.0
21	37.346929537821715	40.0	36.0	40.0	33.0	40.0
22	37.38324056366484	40.0	36.0	40.0	32.0	40.0
23	37.33119484481702	40.0	36.0	40.0	32.0	40.0
24	37.3088303282478	40.0	36.0	40.0	32.0	40.0
25	37.40903328099128	40.0	36.0	40.0	33.0	40.0
26	37.39292435249623	40.0	36.0	40.0	33.0	40.0
27	37.39606589395948	40.0	36.0	40.0	33.0	40.0
28	37.367154038382814	40.0	36.0	40.0	33.0	40.0
29	37.394413147834726	40.0	36.0	40.0	33.0	40.0
30	37.314512597734016	40.0	36.0	40.0	33.0	40.0
31	37.28179626925403	40.0	35.0	40.0	33.0	40.0
32	37.19577505952126	40.0	35.0	40.0	33.0	40.0
33	37.14170235905833	40.0	35.0	40.0	33.0	40.0
34	37.0422264924104	39.0	35.0	40.0	32.0	40.0
35	36.93191001647654	39.0	35.0	40.0	32.0	40.0
36	36.82631094437689	39.0	35.0	40.0	32.0	40.0
37	36.67963914533779	39.0	35.0	40.0	32.0	40.0
38	36.4694404899379	39.0	35.0	40.0	31.0	40.0
39	36.32899067415341	38.0	35.0	40.0	31.0	40.0
40	36.18112590402056	38.0	35.0	40.0	31.0	40.0
41	35.98650308247845	38.0	35.0	40.0	31.0	40.0
42	35.84061437756746	37.0	35.0	40.0	30.0	40.0
43	35.62320748837579	37.0	35.0	40.0	30.0	40.0
44	35.467491410404456	37.0	35.0	40.0	30.0	40.0
45	35.28800806923	36.0	34.0	40.0	30.0	40.0
46	35.10342494210806	36.0	34.0	40.0	29.0	40.0
47	34.933263883372945	35.0	34.0	39.0	29.0	40.0
48	34.75712320341506	35.0	34.0	39.0	29.0	40.0
49	34.60768358923912	35.0	34.0	39.0	29.0	40.0
50	34.438317844566505	35.0	34.0	39.0	28.0	40.0
51	34.2669739980163	35.0	34.0	38.0	28.0	40.0
52	33.61773652192154	35.0	33.0	37.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	6.0
13	4.0
14	13.0
15	12.0
16	58.0
17	124.0
18	300.0
19	601.0
20	1152.0
21	2035.0
22	3362.0
23	4592.0
24	6645.0
25	9205.0
26	12952.0
27	17362.0
28	22338.0
29	29404.0
30	37197.0
31	46843.0
32	58197.0
33	74020.0
34	109389.0
35	193534.0
36	199400.0
37	360652.0
38	561911.0
39	212692.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.04728503607935	19.298789615499764	30.95966199661508	16.6942633518058
2	35.26963285207159	19.281478041796248	28.246632898914665	17.202256207217502
3	26.796075771739776	20.117321553316593	32.345860802727486	20.740741872216145
4	26.322655147341862	18.312080830792603	35.573247304995306	19.79201671687023
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	0.0	100.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	100.0	0.0	0.0
11	99.54434919684482	0.4125246180761343	0.03044800316088969	0.0126781819181631
12	99.57102938690554	0.28559004971476637	0.10239286681697186	0.040987696562736126
13	98.8794829338433	0.729173667670738	0.179225704224635	0.21211769426131513
14	69.25759825336405	21.326280394540948	3.4331905637666726	5.982930788328334
15	34.726915016466364	29.60584601660689	15.488104912209955	20.179134054716794
16	32.33150237983222	15.902258854869949	26.318123588342996	25.448115176954833
17	22.98365991107961	17.951745515793245	29.233850847554283	29.830743725572862
18	23.382997183305125	19.142119873482947	30.448919655968115	27.025963287243815
19	24.831721320323176	20.093289015704652	27.663029199533202	27.411960464438973
20	28.20997309577781	17.84741782603294	26.618784890458468	27.32382418773078
21	30.837004405286343	18.85780273359932	22.501736249009678	27.80345661210466
22	28.66017584485571	20.09023403210991	22.376940169164623	28.87264995386975
23	29.24958401306718	19.045175060743258	23.329229472037735	28.376011454151822
24	30.090570080305334	20.293950521485698	22.18325420925823	27.43222518895074
25	31.003908342345536	18.033873658098457	23.98880043014169	26.97341756941432
26	29.33410522585494	19.47755707218519	23.216042329852687	27.972295372107187
27	30.841281382318975	21.216657399883097	20.78127132123967	27.160789896558256
28	29.738075889865804	21.992368650980342	19.729185887605116	28.540369571548734
29	29.334767138967134	20.5406404467608	21.740077922448222	28.384514491823847
30	29.512516267787642	21.248989309594073	21.513499972505148	27.724994450113137
31	29.098158659554663	19.393697772509626	22.172816348642872	29.335327219292832
32	30.07560066069112	19.392934026610945	22.07200189001652	28.45946342268142
33	28.612416267991307	21.43060808430125	21.57342856735526	28.38354708035218
34	27.52509669023077	21.317930106048664	23.90784336488113	27.24912983883943
35	30.925140681994538	21.481931808692853	19.617118906071475	27.97580860324113
36	31.630994641558775	21.541554905183492	21.096392879036905	25.731057574220827
37	30.739143097468236	22.711104458035727	22.00759265256079	24.54215979193525
38	30.404265979091694	23.160237962855472	21.84791884334248	24.587577214710358
39	30.59265663410054	22.822815024816652	24.01446229233749	22.570066048745318
40	30.634509909348452	23.281113480420608	21.374090887798598	24.71028572243234
41	29.709664542434737	23.907486950128412	20.569510041731075	25.81333846570577
42	30.55732065718807	22.837071614925428	20.43010095702453	26.175506770861972
43	30.809967800472908	21.95881474783147	20.445630456964444	26.78558699473117
44	30.894030765721453	21.866045079337923	19.829287516726033	27.410636638214587
45	28.194698177804117	21.749752037164892	21.12668813301806	28.928861652012927
46	28.848261001505087	22.117470229184867	21.541758570756475	27.492510198553568
47	28.989146661615763	20.374856670353015	24.29058189290857	26.345414775122656
48	28.8527416441107	19.734226610536435	23.0112056798255	28.401826065527363
49	29.12784291681687	20.3785226506667	22.626328663281743	27.867305769234683
50	28.131409100999793	21.550974437933935	22.386308785521823	27.93130767554445
51	28.15264123698322	22.141808265156282	22.4723065737137	27.233243924146795
52	30.103502844189727	21.60199266396606	21.770322260036128	26.52418223180808
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	5.0
10	9.0
11	21.5
12	34.0
13	42.0
14	99.5
15	149.0
16	242.5
17	336.0
18	492.0
19	648.0
20	786.5
21	925.0
22	1180.5
23	1436.0
24	1733.5
25	2031.0
26	2915.5
27	3800.0
28	4464.5
29	5129.0
30	6812.5
31	8496.0
32	9619.0
33	10742.0
34	11934.5
35	13127.0
36	13467.0
37	13807.0
38	15799.5
39	19168.0
40	20544.0
41	26331.5
42	32119.0
43	33729.5
44	35340.0
45	37112.0
46	38884.0
47	44702.0
48	50520.0
49	53803.0
50	57086.0
51	64910.5
52	72735.0
53	82630.5
54	92526.0
55	103679.5
56	114833.0
57	126143.0
58	137453.0
59	156974.5
60	176496.0
61	183614.0
62	190732.0
63	202856.5
64	196425.0
65	177869.0
66	160681.5
67	143494.0
68	125863.5
69	108233.0
70	93848.0
71	79463.0
72	65583.5
73	51704.0
74	48250.5
75	44797.0
76	34876.5
77	24956.0
78	17971.5
79	10987.0
80	8495.0
81	6003.0
82	4266.0
83	2529.0
84	1767.5
85	1006.0
86	566.5
87	127.0
88	95.0
89	36.5
90	10.0
91	6.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1964004.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.995031154125788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.75112357424483	5.088963758994977
2	8.846082961238404	1.2375725181168786
3	4.3452750546752394	0.911860031420968
4	2.5482028914128407	0.7129903444986493
5	1.6647514501875313	0.5822494128968932
6	1.0907773888300034	0.457801309024911
7	0.7681356469344439	0.37611929466307015
8	0.5232979378402454	0.2928388302465837
9	0.38310805960239325	0.24118675310838777
>10	2.641407949438657	3.8906189472139743
>50	0.7566650867744849	3.8958673841235045
>100	3.1616488148445927	55.94157075052183
>500	0.46406846209119806	21.33271815779203
>1k	0.05545472188511171	5.037642507377351
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGACCTCGCAGGGGGGCGTAACCCGAGGCCACCGAAAACCCGAGATGCAGAC	2680	0.13645593389830163	No Hit
TGATCTCGCAGGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATC	2467	0.1256107421369814	No Hit
CCTACTCGCAGGGGGACTGTAGCGGTGAAGGGCCGGAGGGGGCTCTGCGTTC	2297	0.11695495528522344	No Hit
ATGCCTCGCAGGGGGTAACCGCTGGAGGCTTCTGCTGACGGCGTCGGACCTG	2288	0.11649670774601273	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.024388952364659135	0.0	0.0	0.0	0.0
11	0.025610945802554373	0.0	0.0	0.0	0.0
12	0.026629273667467073	0.0	0.0	0.0	0.0
13	0.028513180217555566	0.0	0.0	0.0	0.0
14	0.03711805067606787	0.0	0.0	0.0	0.0
15	0.04903248669554645	0.0	0.0	0.0	0.0
16	0.058706601412217084	0.0	0.0	0.0	0.0
17	0.06563123089362344	0.0	0.0	0.0	0.0
18	0.07581450954275042	0.0	0.0	0.0	0.0
19	0.08808536031494844	0.0	0.0	0.0	0.0
20	0.09770855863837344	0.0	0.0	0.0	0.0
21	0.11018307498355401	0.0	0.0	0.0	0.0
22	0.12250484214899766	0.0	0.0	0.0	0.0
23	0.13518302406716076	0.0	0.0	0.0	0.0
24	0.14613004861497228	0.0	0.0	0.0	0.0
25	0.1595719764318199	0.0	0.0	0.0	0.0
26	0.17973486815709133	0.0	0.0	0.0	0.0
27	0.1971991910403441	0.0	0.0	0.0	0.0
28	0.2142561827776318	0.0	0.0	0.0	0.0
29	0.23599748269351795	0.0	0.0	0.0	0.0
30	0.2613538465298441	0.0	0.0	0.0	0.0
31	0.2847753874228362	0.0	0.0	0.0	0.0
32	0.30509102832784457	0.0	0.0	0.0	0.0
33	0.3188893708974116	0.0	0.0	0.0	0.0
34	0.3341642888711021	0.0	0.0	0.0	0.0
35	0.34964287241777514	0.0	0.0	0.0	0.0
36	0.3643577100657636	0.0	0.0	0.0	0.0
37	0.3785633837812958	0.0	0.0	0.0	0.0
38	0.3956712919118291	0.0	0.0	0.0	0.0
39	0.4123209525031517	0.0	0.0	0.0	0.0
40	0.4331457573406164	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCT	25	3.4180463E-5	46.0	40
AACGTTA	50	1.6370905E-11	46.0	32
GTCATCG	50	1.6370905E-11	46.0	12
AACCGTG	30	1.8620831E-6	46.0	23
CTAGACG	30	1.8620831E-6	46.0	46
TCTTGTA	25	3.4180463E-5	46.0	24
ATTAGTA	50	1.6370905E-11	46.0	24
GGTAATA	30	1.8620831E-6	46.0	13
ATTAGCC	80	0.0	46.0	17
GGTCGTA	95	0.0	46.0	42
GTATTAG	25	3.4180463E-5	46.0	38
CGTATAG	20	6.312554E-4	46.0	36
GATCGAT	20	6.312554E-4	46.0	14
CCTAGTA	60	0.0	46.0	31
GCGAATA	20	6.312554E-4	46.0	16
ACGTTAG	50	1.6370905E-11	46.0	33
CTATCCT	20	6.312554E-4	46.0	31
TTAGCAC	40	5.6152203E-9	46.0	19
GCAACTA	20	6.312554E-4	46.0	13
CGGTTTA	25	3.4180463E-5	46.0	19
>>END_MODULE