FastQCFastQC Report
Fri 17 Jun 2016
SRR1527968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946718
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACCTCGCAGGGGGGCGTAACCCGAGGCCACCGAAAACCCGAGATGCAGAC24680.1267774788130587No Hit
TGATCTCGCAGGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATC23780.12215431305407358No Hit
CCTACTCGCAGGGGGACTGTAGCGGTGAAGGGCCGGAGGGGGCTCTGCGTTC22250.11429493126379887No Hit
ATGCCTCGCAGGGGGTAACCGCTGGAGGCTTCTGCTGACGGCGTCGGACCTG21430.11008271357227908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTT750.046.00000437
CGAATCG750.046.00000422
ATCGCCC1050.046.00000440
GAGATAT1050.046.00000413
AATCGAT1450.046.00000424
CAGCGTT253.4180364E-546.043
CGGGTAT253.4180364E-546.042
ACTATAC253.4180364E-546.027
GTACCAA453.110472E-1046.022
AAATCGC650.046.026
ATTAGTA405.6152203E-946.024
AATTTCG206.3125393E-446.014
CTCAATA600.046.016
GGTAATA301.862074E-646.013
CGTATCT206.3125393E-446.031
ATAGGTC405.6152203E-946.024
CGTATAG253.4180364E-546.036
GATAACG253.4180364E-546.022
GTACACC206.3125393E-446.027
AGATATC800.046.020
Produced by FastQC (version 0.11.5)