##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527968_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1946718
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.888720400181228	31.0	31.0	34.0	30.0	34.0
2	32.21897984196992	34.0	31.0	34.0	30.0	34.0
3	32.33634301424243	34.0	31.0	34.0	30.0	34.0
4	36.02735116231524	37.0	35.0	37.0	35.0	37.0
5	36.07682776858282	37.0	35.0	37.0	35.0	37.0
6	36.09901896422594	37.0	35.0	37.0	35.0	37.0
7	36.03582696620671	37.0	35.0	37.0	35.0	37.0
8	35.779310100384336	37.0	35.0	37.0	33.0	37.0
9	37.32837216278886	39.0	37.0	39.0	34.0	39.0
10	37.34407294739145	39.0	37.0	39.0	34.0	39.0
11	37.88721222077363	39.0	37.0	39.0	35.0	39.0
12	37.986555320287785	39.0	38.0	39.0	35.0	39.0
13	38.06951854351786	39.0	39.0	39.0	37.0	39.0
14	38.76422060103209	40.0	39.0	40.0	36.0	40.0
15	38.09034744631734	40.0	38.0	40.0	34.0	40.0
16	37.4372199774184	40.0	36.0	40.0	33.0	40.0
17	37.38451588776597	40.0	36.0	40.0	33.0	40.0
18	37.426908776720616	40.0	37.0	40.0	33.0	40.0
19	37.51353817039756	40.0	37.0	40.0	33.0	40.0
20	37.56518663720169	40.0	37.0	40.0	33.0	40.0
21	37.57148133422509	40.0	37.0	40.0	33.0	40.0
22	37.511085324119875	40.0	37.0	40.0	33.0	40.0
23	37.427106031792995	40.0	36.0	40.0	33.0	40.0
24	37.403987120887564	40.0	36.0	40.0	33.0	40.0
25	37.30476216894281	40.0	36.0	40.0	32.0	40.0
26	37.30766757177979	40.0	36.0	40.0	33.0	40.0
27	37.34724700752754	40.0	36.0	40.0	33.0	40.0
28	37.17121329334809	40.0	36.0	40.0	32.0	40.0
29	37.30546026697241	40.0	36.0	40.0	33.0	40.0
30	37.29271779477048	40.0	36.0	40.0	33.0	40.0
31	37.30476627842348	40.0	36.0	40.0	33.0	40.0
32	37.2159151967568	40.0	35.0	40.0	33.0	40.0
33	37.1509222188319	40.0	35.0	40.0	33.0	40.0
34	37.07622007912805	40.0	35.0	40.0	32.0	40.0
35	36.89949905430576	39.0	35.0	40.0	32.0	40.0
36	36.432851085776164	39.0	35.0	40.0	31.0	40.0
37	36.48703458847147	39.0	35.0	40.0	31.0	40.0
38	36.39621814767213	39.0	35.0	40.0	31.0	40.0
39	36.29051870892446	38.0	35.0	40.0	31.0	40.0
40	36.151463642910784	38.0	35.0	40.0	31.0	40.0
41	35.991536524550554	38.0	35.0	40.0	31.0	40.0
42	35.70148115957216	37.0	35.0	40.0	30.0	40.0
43	35.490606240862824	37.0	35.0	40.0	30.0	40.0
44	35.33877531311674	37.0	34.0	40.0	29.0	40.0
45	34.93483596494202	36.0	34.0	40.0	28.0	40.0
46	34.900295779871556	36.0	34.0	40.0	28.0	40.0
47	34.803530865795665	35.0	34.0	39.0	28.0	40.0
48	34.62847572170186	35.0	34.0	39.0	28.0	40.0
49	34.48107275938271	35.0	34.0	39.0	28.0	40.0
50	34.24475501844643	35.0	34.0	39.0	27.0	40.0
51	34.16388968510077	35.0	34.0	38.0	27.0	40.0
52	33.6515910368117	35.0	33.0	37.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	3.0
13	4.0
14	11.0
15	13.0
16	46.0
17	129.0
18	319.0
19	645.0
20	1180.0
21	2035.0
22	3238.0
23	4725.0
24	6770.0
25	9617.0
26	12995.0
27	17529.0
28	23535.0
29	29992.0
30	37778.0
31	47059.0
32	59344.0
33	75432.0
34	110041.0
35	191829.0
36	201525.0
37	359628.0
38	541373.0
39	209919.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.08116532543492	19.34137353227329	30.877559050668868	16.699902091622924
2	35.32997588762214	19.334541520651683	28.112700452762034	17.222782138964142
3	26.766742794796166	20.149965223519793	32.383837823454655	20.69945415822939
4	26.236517050749004	18.34138277860481	35.652518752074	19.769581418572184
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	0.0	100.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	100.0	0.0	0.0
11	99.5344472080702	0.41711228847732434	0.03451963766708892	0.013920865785388536
12	99.56747715899272	0.2903861781726989	0.10248017432417023	0.03965648851040572
13	98.87431050619556	0.7355456722545329	0.17901925188959059	0.2111245696603206
14	69.25019442980442	21.322605534032153	3.4237110870706493	6.00348894909278
15	34.76204565838504	29.573826306635066	15.462280617942609	20.201847417037293
16	32.26964562920772	15.987215405621152	26.335761009041885	25.40737795612924
17	23.00328039294854	17.967985090804113	29.216147382414913	29.81258713383243
18	23.37328775919265	19.17185745444384	30.502106622530846	26.95274816383267
19	24.891997711019265	20.128339081469427	27.664202005632042	27.315461201879266
20	28.161962852349443	17.876446408776207	26.552536114629856	27.4090546242445
21	30.46306655612164	19.015697188807007	22.53916591925487	27.982070335816488
22	28.664963286927026	20.130958875399518	22.369855315459148	28.834222522214308
23	29.289861192016513	19.018316982737097	23.352123933718186	28.339697891528203
24	30.078778744533107	20.33329943011777	22.135717654020766	27.452204171328358
25	31.061201468317446	18.097741943106293	23.898736231955528	26.94232035662073
26	29.350219189425488	19.474623443148932	23.187076916122418	27.988080451303166
27	30.860812916919656	21.265226910112304	20.743322864431317	27.130637308536727
28	29.842432237232103	22.004573851990887	19.72545586982809	28.427538040948917
29	29.345698760683366	20.56651245840435	21.72682432689275	28.360964454019534
30	29.522509166710332	21.29887328313603	21.4321745625201	27.746442987633547
31	29.106064668842635	19.33628804993841	22.251245429486964	29.306401851731994
32	30.14108874526254	19.429932840812075	22.028819787971344	28.40015862595404
33	28.64806304765251	21.449691223895808	21.560493096586153	28.34175263186553
34	27.48631286092798	21.26193932557258	23.915996050789072	27.335751762710363
35	30.910486264574534	21.43299645865503	19.628831705465302	28.02768557130514
36	31.701407188920015	21.460838190225807	21.189920676749278	25.647833944104896
37	30.705217704875594	22.678631419650923	22.035754536609822	24.580396338863668
38	30.37168197961903	23.210346850442644	21.858481814006957	24.55948935593137
39	30.56914252603613	22.87398585722226	24.065529778838023	22.49134183790359
40	30.63741127374381	23.27645812079613	21.372432987212324	24.71369761824774
41	29.79440268184709	23.917896685601097	20.54426989425279	25.743430738299022
42	30.67727323628795	22.8093129051049	20.441738351420184	26.07167550718697
43	30.75910327022198	21.966201576191313	20.520332169322934	26.754362984263768
44	31.008035062089117	21.81373984316167	19.758074872683153	27.420150222066063
45	28.255196695155643	21.75892964466348	21.25818942445696	28.72768423572392
46	28.871516059336795	22.140597662321916	21.541435379957445	27.446450898383844
47	28.997933958590817	20.353538622440436	24.334803500044693	26.313723918924055
48	28.938500594333643	19.71893206925708	23.044837516270974	28.297729820138308
49	29.198065667446443	20.352819463322373	22.67893963070152	27.77017523852967
50	28.21805726355846	21.508867745610818	22.41690886918393	27.85616612164679
51	28.090560625627337	22.096523482086262	22.576253982343616	27.236661909942782
52	30.121157763990468	21.535836212538232	21.813483000619506	26.52952302285179
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	2.0
10	4.0
11	10.5
12	17.0
13	38.5
14	102.5
15	145.0
16	260.0
17	375.0
18	494.5
19	614.0
20	768.0
21	922.0
22	1174.5
23	1427.0
24	1683.0
25	1939.0
26	2851.5
27	3764.0
28	4292.5
29	4821.0
30	6536.0
31	8251.0
32	9342.5
33	10434.0
34	11734.5
35	13035.0
36	13489.0
37	13943.0
38	15734.5
39	18917.0
40	20308.0
41	26023.0
42	31738.0
43	33684.5
44	35631.0
45	37108.5
46	38586.0
47	44320.5
48	50055.0
49	53523.5
50	56992.0
51	64790.5
52	72589.0
53	82312.5
54	92036.0
55	102991.5
56	113947.0
57	125402.5
58	136858.0
59	155758.0
60	174658.0
61	182400.5
62	190143.0
63	201236.0
64	194021.0
65	175713.0
66	158960.0
67	142207.0
68	124690.5
69	107174.0
70	92563.0
71	77952.0
72	64559.0
73	51166.0
74	47750.0
75	44334.0
76	34508.0
77	24682.0
78	17724.0
79	10766.0
80	8405.5
81	6045.0
82	4237.0
83	2429.0
84	1681.5
85	934.0
86	523.5
87	113.0
88	81.0
89	27.5
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1946718.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.1015783768629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.82382009133521	5.980894645493782
2	9.308675433662023	1.5082992722118223
3	4.2424454750228895	1.0311151357639587
4	2.526980376926987	0.8189011832187416
5	1.5324131948316366	0.620748280183369
6	0.9081342944985112	0.441439269815807
7	0.6699439023025844	0.3799322122824038
8	0.449721362997079	0.2914762296056357
9	0.3138991143796259	0.22887704498169525
>10	2.321610233425379	4.006890547111583
>50	0.676859703376045	4.07101671372129
>100	2.811571075562033	56.59978158324223
>500	0.37097118358110154	19.58732237019052
>1k	0.04295455809886439	4.4333055121771565
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGACCTCGCAGGGGGGCGTAACCCGAGGCCACCGAAAACCCGAGATGCAGAC	2468	0.1267774788130587	No Hit
TGATCTCGCAGGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATC	2378	0.12215431305407358	No Hit
CCTACTCGCAGGGGGACTGTAGCGGTGAAGGGCCGGAGGGGGCTCTGCGTTC	2225	0.11429493126379887	No Hit
ATGCCTCGCAGGGGGTAACCGCTGGAGGCTTCTGCTGACGGCGTCGGACCTG	2143	0.11008271357227908	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.02470825255635382	0.0	0.0	0.0	0.0
11	0.025632885708150848	0.0	0.0	0.0	0.0
12	0.026557518859947872	0.0	0.0	0.0	0.0
13	0.027995837096076576	0.0	0.0	0.0	0.0
14	0.03523879678515327	0.0	0.0	0.0	0.0
15	0.047002185216348746	0.0	0.0	0.0	0.0
16	0.056608096293351166	0.0	0.0	0.0	0.0
17	0.06323463388122984	0.0	0.0	0.0	0.0
18	0.07320012451726444	0.0	0.0	0.0	0.0
19	0.0853230925074921	0.0	0.0	0.0	0.0
20	0.09523721463509352	0.0	0.0	0.0	0.0
21	0.107000603066289	0.0	0.0	0.0	0.0
22	0.12087010034324437	0.0	0.0	0.0	0.0
23	0.1325307517575735	0.0	0.0	0.0	0.0
24	0.14388319212130365	0.0	0.0	0.0	0.0
25	0.15893416509222189	0.0	0.0	0.0	0.0
26	0.17855693531369207	0.0	0.0	0.0	0.0
27	0.1960222281809692	0.0	0.0	0.0	0.0
28	0.21251151938801613	0.0	0.0	0.0	0.0
29	0.23208292110105316	0.0	0.0	0.0	0.0
30	0.2578699123345035	0.0	0.0	0.0	0.0
31	0.28211584831495884	0.0	0.0	0.0	0.0
32	0.2996325096906691	0.0	0.0	0.0	0.0
33	0.31309105890015915	0.0	0.0	0.0	0.0
34	0.32778245231204517	0.0	0.0	0.0	0.0
35	0.3412923700299684	0.0	0.0	0.0	0.0
36	0.35665155405148563	0.0	0.0	0.0	0.0
37	0.37190800105613653	0.0	0.0	0.0	0.0
38	0.3886541348053493	0.0	0.0	0.0	0.0
39	0.40694132380755715	0.0	0.0	0.0	0.0
40	0.4264099885037278	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTT	75	0.0	46.000004	37
CGAATCG	75	0.0	46.000004	22
ATCGCCC	105	0.0	46.000004	40
GAGATAT	105	0.0	46.000004	13
AATCGAT	145	0.0	46.000004	24
CAGCGTT	25	3.4180364E-5	46.0	43
CGGGTAT	25	3.4180364E-5	46.0	42
ACTATAC	25	3.4180364E-5	46.0	27
GTACCAA	45	3.110472E-10	46.0	22
AAATCGC	65	0.0	46.0	26
ATTAGTA	40	5.6152203E-9	46.0	24
AATTTCG	20	6.3125393E-4	46.0	14
CTCAATA	60	0.0	46.0	16
GGTAATA	30	1.862074E-6	46.0	13
CGTATCT	20	6.3125393E-4	46.0	31
ATAGGTC	40	5.6152203E-9	46.0	24
CGTATAG	25	3.4180364E-5	46.0	36
GATAACG	25	3.4180364E-5	46.0	22
GTACACC	20	6.3125393E-4	46.0	27
AGATATC	80	0.0	46.0	20
>>END_MODULE