FastQCFastQC Report
Fri 17 Jun 2016
SRR1527967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1941181
Sequences flagged as poor quality0
Sequence length59
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACCTCGCAGGGGGGCGTAACCCGAGGCCACCGAAAACCCGAGATGCAGACGGCGAGA24730.12739667243806735No Hit
TGATCTCGCAGGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTGTC24330.12533607118553086No Hit
CCTACTCGCAGGGGGACTGTAGCGGTGAAGGGCCGGAGGGGGCTCTGCGTTCTGGAGTG21860.11261185845111817No Hit
ATGCCTCGCAGGGGGTAACCGCTGGAGGCTTCTGCTGACGGCGTCGGACCTGCGGAGCC21740.11199367807535722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA600.053.00000432
AACGTAT307.0130045E-753.00000447
GATACGC150.006696957653.00000434
ACACGAT150.006696957653.00000419
ATTAGTA750.053.00000424
GTCGATA457.6397555E-1153.00000450
CTCAATA457.6397555E-1153.00000416
CGTATCT307.0130045E-753.00000431
GTATTCG307.0130045E-753.00000433
ACGTTAG600.053.00000433
CGCATTG900.053.00000446
TTATGCG307.0130045E-753.00000437
CGCATAT150.006696957653.00000439
CCAATTG150.006696957653.00000417
CGACCTA750.053.00000445
CGATTTA150.006696957653.00000438
CACGATA150.006696957653.00000431
TAACGTT150.006696957653.00000432
CAATTAG150.006696957653.00000436
TAACGTA150.006696957653.00000436