##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527967_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1941181
Sequences flagged as poor quality	0
Sequence length	59
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51172971505491	31.0	31.0	34.0	30.0	34.0
2	31.96286745027898	31.0	31.0	34.0	30.0	34.0
3	32.0726006487803	33.0	31.0	34.0	30.0	34.0
4	35.84924795781537	37.0	35.0	37.0	35.0	37.0
5	35.80750275219055	37.0	35.0	37.0	35.0	37.0
6	35.86376437848918	37.0	35.0	37.0	35.0	37.0
7	35.76053186178929	37.0	35.0	37.0	33.0	37.0
8	35.4775391887722	37.0	35.0	37.0	32.0	37.0
9	36.95503201401621	39.0	37.0	39.0	32.0	39.0
10	36.79777310822639	39.0	37.0	39.0	32.0	39.0
11	37.37585675936453	39.0	37.0	39.0	35.0	39.0
12	37.64603352289148	39.0	37.0	39.0	35.0	39.0
13	37.75800298890211	39.0	37.0	39.0	35.0	39.0
14	38.21292398802585	40.0	38.0	40.0	35.0	40.0
15	37.455954390651875	40.0	37.0	40.0	32.0	40.0
16	36.789283946216244	39.0	35.0	40.0	31.0	40.0
17	36.61633201643742	39.0	35.0	40.0	31.0	40.0
18	36.601904716767784	39.0	35.0	40.0	30.0	40.0
19	36.81593833856812	39.0	35.0	40.0	31.0	40.0
20	37.020853799825986	39.0	36.0	40.0	31.0	40.0
21	37.01175985134822	39.0	36.0	40.0	31.0	40.0
22	36.9749487554226	39.0	35.0	40.0	31.0	40.0
23	36.91117572240816	39.0	35.0	40.0	31.0	40.0
24	36.88873268386616	39.0	35.0	40.0	31.0	40.0
25	36.74508044329715	39.0	35.0	40.0	31.0	40.0
26	36.55107895657334	39.0	35.0	40.0	30.0	40.0
27	36.441585302967624	39.0	35.0	40.0	30.0	40.0
28	36.4182392059267	39.0	35.0	40.0	30.0	40.0
29	36.4039777846579	39.0	35.0	40.0	30.0	40.0
30	36.32693190382556	39.0	35.0	40.0	30.0	40.0
31	36.32503048401978	39.0	35.0	40.0	30.0	40.0
32	36.163596800092314	39.0	35.0	40.0	30.0	40.0
33	36.0453332275558	38.0	35.0	40.0	30.0	40.0
34	35.952682413438005	38.0	35.0	40.0	30.0	40.0
35	35.8185182113363	38.0	35.0	40.0	29.0	40.0
36	35.602194231243764	38.0	34.0	40.0	28.0	40.0
37	35.44207366546448	38.0	34.0	40.0	28.0	40.0
38	35.20582264095929	38.0	34.0	40.0	27.0	40.0
39	35.04887694655985	37.0	34.0	40.0	27.0	40.0
40	34.868761851676894	37.0	33.0	40.0	26.0	40.0
41	34.69126681128653	37.0	33.0	40.0	26.0	40.0
42	34.568571915756436	36.0	33.0	40.0	26.0	40.0
43	34.3043848049203	36.0	33.0	39.0	26.0	40.0
44	34.16835730413599	36.0	33.0	39.0	25.0	40.0
45	33.95011490427734	35.0	33.0	39.0	24.0	40.0
46	33.7008790009793	35.0	33.0	39.0	24.0	40.0
47	33.54758057079685	35.0	33.0	39.0	23.0	40.0
48	33.34262801871644	35.0	32.0	38.0	23.0	40.0
49	33.24398909735877	35.0	32.0	38.0	24.0	40.0
50	33.013327453751096	35.0	32.0	38.0	23.0	40.0
51	32.831753453181335	35.0	32.0	37.0	23.0	40.0
52	32.61281972160247	35.0	31.0	37.0	23.0	40.0
53	32.33415997786914	35.0	31.0	37.0	22.0	39.0
54	32.18255484676596	35.0	31.0	36.0	22.0	39.0
55	32.058058470590844	34.0	31.0	36.0	22.0	39.0
56	31.870139878764526	34.0	31.0	36.0	21.0	39.0
57	31.74014015179419	34.0	31.0	35.0	21.0	39.0
58	31.580512584864575	34.0	31.0	35.0	20.0	38.0
59	31.122568683703374	34.0	30.0	35.0	20.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	11.0
14	25.0
15	55.0
16	252.0
17	648.0
18	1341.0
19	2542.0
20	4211.0
21	6273.0
22	8790.0
23	11777.0
24	16269.0
25	20773.0
26	26145.0
27	32742.0
28	39916.0
29	47998.0
30	57115.0
31	69009.0
32	83347.0
33	105902.0
34	149766.0
35	205846.0
36	258973.0
37	365084.0
38	359691.0
39	66676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.7478478307793	19.451303098474586	30.81557052124454	16.98527854950157
2	35.12681197683266	19.915865650858937	27.857834998385005	17.0994873739234
3	25.854312400543794	20.55357022348766	32.58366942598346	21.008447949985086
4	25.4733072289498	18.70407756927355	35.85121634716186	19.971398854614794
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	0.0	100.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	100.0	0.0	0.0
11	99.49669814406796	0.4499322834913385	0.03317568016583719	0.02019389227485742
12	99.56114344824104	0.28791751001065846	0.10493611878541981	0.046002922962876724
13	98.86182689816148	0.7420225110383833	0.16938142295849792	0.2267691678416387
14	68.92443311571667	21.56414059276286	3.5168281577039955	5.994598133816476
15	34.67620999793425	29.720773075771916	15.541209191724006	20.061807734569832
16	32.245267185285655	16.0839200466108	26.248711480279276	25.422101287824265
17	23.026085666406175	18.109181987666272	29.16229862130322	29.70243372462434
18	23.17975500481408	19.293976192843427	30.40247148514229	27.1237973172002
19	24.701818120000144	20.240770953352623	27.667486957681948	27.389923968965284
20	28.144310087518885	17.940830865334043	26.605607617218592	27.309251429928484
21	30.49504399641249	19.068494900784625	22.49125661131033	27.945204491492547
22	28.66342705806414	20.295995066920604	22.30776006977196	28.732817805243304
23	29.17048951128205	19.157152269675006	23.36680608351308	28.305552135529865
24	29.980717923779387	20.381046383619044	22.26505410881314	27.373181583788426
25	31.060833585327696	18.074666916686287	24.03722270102582	26.827276796960202
26	29.255798403137057	19.571899786779284	23.280569921094425	27.89173188898923
27	30.75514338951391	21.372607706339593	20.828763520763907	27.043485383382592
28	29.637164179950247	22.065639422598924	19.84796883958786	28.449227557862972
29	29.33219519457485	20.60292162348591	21.74459774745374	28.320285434485506
30	29.393910202088314	21.423040921995423	21.459152958946127	27.723895916970132
31	28.998120216507374	19.602036080097633	22.23728750693521	29.162556196459782
32	30.01317239350684	19.39504868428034	22.164136162470164	28.42764275974265
33	28.477663855147973	21.619467736393464	21.611688966665138	28.291179441793425
34	27.346290737442825	21.41211973535698	24.064268092465362	27.177321434734836
35	30.97181561121812	21.491916518861455	19.58812702164301	27.948140848277415
36	31.588759626227535	21.80600366477933	21.124974950816025	25.480261758177107
37	30.774049406005933	22.798595288126148	21.991663837632863	24.43569146823506
38	30.388407881593732	23.273151756585296	21.897082240141437	24.441358121679535
39	30.518483335660097	22.934852545950122	24.04618631647435	22.50047780191543
40	30.4735622283548	23.469578570983334	21.430613631598497	24.626245569063368
41	29.677088329218144	24.11985281125253	20.61337917484253	25.589679684686793
42	30.567628675533093	22.94979190503101	20.332725284247065	26.14985413518884
43	30.607810399957554	22.2287875267685	20.499788530796458	26.663613542477492
44	30.85327952416596	21.87884591905649	19.817162850862438	27.45071170591511
45	28.052304241593134	21.795855203610586	21.26143826876525	28.89040228603103
46	28.715199664534115	22.28262073449101	21.523031597774754	27.479148003200116
47	28.80797823592957	20.529049068582474	24.34538561834265	26.317587077145305
48	28.875102321730946	19.84271430639389	22.953397957222947	28.328785414652213
49	29.148544107942538	20.479027973177153	22.620868430094877	27.75155948878544
50	28.09392838689437	21.610710181070182	22.36968113741068	27.925680294624765
51	28.03885881842033	22.35396905286009	22.545244364126788	27.06192776459279
52	29.85265155593425	21.85870354181295	21.786221892754977	26.50242300949783
53	27.68345661738911	24.09929831375848	21.91655492197791	26.300690146874505
54	26.545953210957656	23.371957586644417	23.308903188316805	26.77318601408112
55	26.892803916790857	22.619374494186786	22.84985274428299	27.637968844739362
56	27.845007755587964	23.800871737359884	21.75577650924875	26.598343997803397
57	28.168522152236193	23.869747334225917	21.480119576690683	26.481610936847204
58	28.08383144075694	22.87344662862453	21.182671785887045	27.86005014473148
59	27.11426703640722	21.643782831173393	24.046237831505664	27.195712300913723
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	3.5
9	12.5
10	18.0
11	28.5
12	39.0
13	67.0
14	171.5
15	248.0
16	320.0
17	392.0
18	453.0
19	596.5
20	679.0
21	737.0
22	795.0
23	996.0
24	1197.0
25	1941.5
26	3068.0
27	3450.0
28	3750.5
29	4051.0
30	5968.5
31	7907.5
32	7929.0
33	9778.0
34	11627.0
35	12814.5
36	15611.5
37	17221.0
38	18683.5
39	20146.0
40	21445.5
41	22745.0
42	26951.0
43	32116.5
44	33076.0
45	35516.0
46	37956.0
47	39155.5
48	44097.5
49	47840.0
50	55438.5
51	63037.0
52	71063.5
53	85758.5
54	92427.0
55	99836.5
56	107246.0
57	115867.5
58	140687.5
59	156886.0
60	168266.5
61	179647.0
62	176568.5
63	173490.0
64	164197.0
65	138864.0
66	122824.0
67	116254.5
68	109685.0
69	99947.0
70	75499.0
71	60789.0
72	55061.0
73	49333.0
74	39646.0
75	24115.0
76	18271.0
77	15048.0
78	11825.0
79	8774.5
80	5724.0
81	4229.5
82	2279.5
83	1824.0
84	1056.0
85	288.0
86	242.5
87	150.5
88	104.0
89	91.0
90	78.0
91	43.0
92	4.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1941181.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.717528088698973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.0519769484767	9.497288165383573
2	7.18704062469024	1.6842870078885699
3	3.0475597970442214	1.0712960257156618
4	1.776780434613789	0.8327789860015138
5	1.073163319854072	0.6287410672075764
6	0.6941600960735697	0.4880304254277618
7	0.44493091377665517	0.364944333579592
8	0.32549451567155085	0.3051192904079092
9	0.22628824997602423	0.23863850327129382
>10	1.5548835362241311	3.788753312949438
>50	0.48375247997554194	4.148905827169926
>100	1.861940846478477	54.11181025796482
>500	0.2451775535433834	18.764721202175007
>1k	0.026850683601699076	4.074685594857352
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGACCTCGCAGGGGGGCGTAACCCGAGGCCACCGAAAACCCGAGATGCAGACGGCGAGA	2473	0.12739667243806735	No Hit
TGATCTCGCAGGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTGTC	2433	0.12533607118553086	No Hit
CCTACTCGCAGGGGGACTGTAGCGGTGAAGGGCCGGAGGGGGCTCTGCGTTCTGGAGTG	2186	0.11261185845111817	No Hit
ATGCCTCGCAGGGGGTAACCGCTGGAGGCTTCTGCTGACGGCGTCGGACCTGCGGAGCC	2174	0.11199367807535722	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.020812072650618362	0.0	0.0	0.0	0.0
11	0.02127570793243907	0.0	0.0	0.0	0.0
12	0.0223060085587073	0.0	0.0	0.0	0.0
13	0.023748429435482832	0.0	0.0	0.0	0.0
14	0.03065144363148001	0.0	0.0	0.0	0.0
15	0.0428605060527586	0.0	0.0	0.0	0.0
16	0.05136048621947155	0.0	0.0	0.0	0.0
17	0.058727135697289434	0.0	0.0	0.0	0.0
18	0.06784529623976332	0.0	0.0	0.0	0.0
19	0.07850890772163956	0.0	0.0	0.0	0.0
20	0.08906948914088897	0.0	0.0	0.0	0.0
21	0.1013815816247944	0.0	0.0	0.0	0.0
22	0.11256034341980475	0.0	0.0	0.0	0.0
23	0.12332698496430781	0.0	0.0	0.0	0.0
24	0.13445423172800475	0.0	0.0	0.0	0.0
25	0.14753904968161136	0.0	0.0	0.0	0.0
26	0.1656723407039323	0.0	0.0	0.0	0.0
27	0.1815389703484631	0.0	0.0	0.0	0.0
28	0.19730256993036713	0.0	0.0	0.0	0.0
29	0.21517828579612103	0.0	0.0	0.0	0.0
30	0.23799944466796244	0.0	0.0	0.0	0.0
31	0.2592236375690881	0.0	0.0	0.0	0.0
32	0.2746266319317982	0.0	0.0	0.0	0.0
33	0.288484175355106	0.0	0.0	0.0	0.0
34	0.30141444821477237	0.0	0.0	0.0	0.0
35	0.3137265406986778	0.0	0.0	0.0	0.0
36	0.32753256909067213	0.0	0.0	0.0	0.0
37	0.34061738704427874	0.0	0.0	0.0	0.0
38	0.3566385617827498	0.0	0.0	0.0	0.0
39	0.3733809469596086	0.0	0.0	0.0	0.0
40	0.39362635426577947	0.0	0.0	0.0	0.0
41	0.40980207409819075	0.0	0.0	0.0	0.0
42	0.42546264361746794	0.0	0.0	0.0	0.0
43	0.4388050367276416	0.0	0.0	0.0	0.0
44	0.45173530958730795	0.0	0.0	0.0	0.0
45	0.46461406741566086	0.0	0.0	0.0	0.0
46	0.4796049415278637	0.0	0.0	0.0	0.0
47	0.4918655189804557	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTA	60	0.0	53.000004	32
AACGTAT	30	7.0130045E-7	53.000004	47
GATACGC	15	0.0066969576	53.000004	34
ACACGAT	15	0.0066969576	53.000004	19
ATTAGTA	75	0.0	53.000004	24
GTCGATA	45	7.6397555E-11	53.000004	50
CTCAATA	45	7.6397555E-11	53.000004	16
CGTATCT	30	7.0130045E-7	53.000004	31
GTATTCG	30	7.0130045E-7	53.000004	33
ACGTTAG	60	0.0	53.000004	33
CGCATTG	90	0.0	53.000004	46
TTATGCG	30	7.0130045E-7	53.000004	37
CGCATAT	15	0.0066969576	53.000004	39
CCAATTG	15	0.0066969576	53.000004	17
CGACCTA	75	0.0	53.000004	45
CGATTTA	15	0.0066969576	53.000004	38
CACGATA	15	0.0066969576	53.000004	31
TAACGTT	15	0.0066969576	53.000004	32
CAATTAG	15	0.0066969576	53.000004	36
TAACGTA	15	0.0066969576	53.000004	36
>>END_MODULE